Scopolamine-induced learning impairment reversed by physostigmine in zebrafish

Kim, Yeon-Hwa; Lee, Yunkyoung; Kim, Do-Hee; Jung, Min Whan; Lee, Chang‐Joong

doi:10.1016/j.neures.2010.03.003

Cited by 82 publications

(61 citation statements)

References 33 publications

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“…Further, the results indicate no anxiolytic effect of physostigmine in the presence of scopolamine, suggesting the involvement of muscarinic receptors in the anxiolytic effect of physostigmine on zebrafish. Similarly, the scopolamine-induced learning impairment was rescued by physostigmine in a passive avoidance response learning in the zebrafish (Kim et al 2010). Thus, further research is needed to clarify the effect of muscarinic and nicotinic receptors on the anxiolytic action of physostigmine.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, further research is needed to clarify the effect of muscarinic and nicotinic receptors on the anxiolytic action of physostigmine. However, the anxiolytic effect of physostigmine has important implications in that the corresponding doses can have positive effects on cognitive functions such as learning and memory in zebrafish (Kim et al 2010). Of interest, physostigmine exerted anxiolytic-like effects in the open field in rats (Sienkiewicz-Jarosz et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Histologically, neurons containing AChE are detected in most areas of the central nervous system, including the olfactory bulb, telencephalon, cerebellum, medulla oblongata, and spinal cord (Clemente et al 2004). Behaviorally, it has been reported that in zebrafish, scopolamine-induced learning deficits associated with passive avoidance tasks can be reduced by pretreatment with physostigmine (Kim et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Anxiolytic effects of an acetylcholinesterase inhibitor, physostigmine, in the adult zebrafish

Cho

Lee

Choi

et al. 2012

Animal Cells and Systems

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Anxiety in zebrafish can be determined by examining their bottom-dwelling and light-avoidance behavior. This study determines the effects of physostigmine and scopolamine on anxiety in zebrafish by measuring swimming frequency for three horizontal layers and three vertical columns of a water test tank illuminated by a light source located above the central surface of the tank. In the 1 h session, zebrafish in the control group preferred the bottom layer the most and the center column the least. Zebrafish treated with 2Á20 mM physostigmine were more likely to prefer the top layer than controls, and there were significant pairwise differences between physostigmine-treated zebrafish and controls, indicating the anxiolytic effect of physostigmine. Further, 10 and 20 mM physostigmine-treated zebrafish no longer avoided the center column. Scopolamine had no anxiolytic effect on bottom-dwelling and light-avoidance behaviors but suppressed the anxiolytic effect of physostigmine. In terms of their preference for various zones formed by layers and columns, zebrafish in the control group preferred the bottom left and right zones the most. Physostigmine had a positive effect on the preference for the top center zone, which was suppressed by scopolamine pretreatment. The results suggest that the level of anxiety in zebrafish can be reduced by activating acetylcholinergic neurotransmitter systems, which is mediated in part by muscarinic receptors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anxiolytic effects of an acetylcholinesterase inhibitor, physostigmine, in the adult zebrafish

Cho

Lee

Choi

et al. 2012

Animal Cells and Systems

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“…Anxiolytics, including nicotine (Levin et al, 2007;Bencan and Levin, 2008), buspirone, diazepam, and fluoxetine (Bencan et al, 2009;Maximino et al, 2011), and anxiogenics such as caffeine (Egan et al, 2009) alter adult zebrafish anxiety-related behavioral responses as assessed by either a novel tank or light/dark preference paradigms. Drugs that affect adult zebrafish learning and memory tests include nicotine (Levin and Chen, 2004;Levin et al, 2006), the anti-cholinergic scopolamine (Kim et al, 2010;Richetti et al, 2011), the antihistaminerigc a-fluoromethylhistidine (Peitsaro et al, 2003), the NMDA receptor antagonist MK-801 (Swain et al, 2004;Blank et al, 2009), environmental neurotoxins , and melatonin (Rawashdeh et al, 2007). Some work has also been done on the effects of neuroactive drugs on larval zebrafish locomotor behavior.…”

Section: Probing Zebrafish Behavior With Small Moleculesmentioning

confidence: 99%

Behavioral screening for neuroactive drugs in zebrafish

Rihel

Schier

2012

Developmental Neurobiology

110

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The larval zebrafish has emerged asa vertebrate model system amenable to small molecule screens for probing diverse biological pathways. Two large-scale small molecule screens examined the effects of thousands of drugs on larval zebrafish sleep/wake and photomotor response behaviors. Both screens identified hundreds of molecules that altered zebrafish behavior in distinct ways. The behavioral profiles induced by these small molecules enabled the clustering of compounds according to shared phenotypes. This approach identified regulators of sleep/wake behavior and revealed the biological targets for poorly characterized compounds. Behavioral screening for neuroactive small molecules in zebrafish is an attractive complement to in vitro screening efforts, because the complex interactions in the vertebrate brain can only be revealed in vivo.

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“…In addition, instruments are developed for measuring passive or active avoidance conditioning in zebrafish. Using these paradigms it was shown that arsenic had an adverse effect on long term memory, and scopolamine exposure resulted in an impaired acquisition and retention of the response which could be rescued by physostigmine (de Castro et al, 2009;Kim et al, 2010).…”

Section: Learning and Memorymentioning

confidence: 99%