<scp>20E</scp> biosynthesis gene <scp><i>CYP306A1</i></scp> confers resistance to imidacloprid in the nymph stage of <scp><i>Bemisia tabaci</i></scp> by detoxification metabolism

Liu, Shaonan; Fu, Buli; Zhang, CJ; He, Chao; Gong, Pan; Huang, Mingjiao; Du, Taisheng; Liang, Jinjin; Wei, Xuegao; Yang, Jing; Chen, Yin; Ji, Yao; Xue, Hong; Hu, Jinyu; Wang, Chao; Zhang, Rong; Du, He; Yang, Xin; Zhang, Youjun

doi:10.1002/ps.7569

Cited by 10 publications

(1 citation statement)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The whitefly, often termed a "super pest", has become a significant subject of research due to concerns surrounding its resistance mechanisms [27,28]. This insect's rapid adaptability and resistance to predominant insecticide classifications pose a formidable threat to global agricultural stability [29].…”

Section: Discussionmentioning

confidence: 99%

Managing Super Pests: Interplay between Pathogens and Symbionts Informs Biocontrol of Whiteflies

Yan,

Wang,

Liu

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Bemisia tabaci is distributed globally and incurs considerable economic and ecological costs as an agricultural pest and viral vector. The entomopathogenic fungus Metarhizium anisopliae has been known for its insecticidal activity, but its impacts on whiteflies are understudied. We investigated how infection with the semi-persistently transmitted Cucurbit chlorotic yellows virus (CCYV) affects whitefly susceptibility to M. anisopliae exposure. We discovered that viruliferous whiteflies exhibited increased mortality when fungus infection was present compared to non-viruliferous insects. High throughput 16S rRNA sequencing also revealed significant alterations of the whitefly bacterial microbiome diversity and structure due to both CCYV and fungal presence. Specifically, the obligate symbiont Portiera decreased in relative abundance in viruliferous whiteflies exposed to M. anisopliae. Facultative Hamiltonella and Rickettsia symbionts exhibited variability across groups but dominated in fungus-treated non-viruliferous whiteflies. Our results illuminate triangular interplay between pest insects, their pathogens, and symbionts—dynamics which can inform integrated management strategies leveraging biopesticides This work underscores the promise of M. anisopliae for sustainable whitefly control while laying the groundwork for elucidating mechanisms behind microbe-mediated shifts in vector competence.

show abstract

Section: Discussionmentioning

confidence: 99%

Managing Super Pests: Interplay between Pathogens and Symbionts Informs Biocontrol of Whiteflies

Yan,

Wang,

Liu

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

Is insecticide resistance a factor contributing to the increasing problems with Dalbulus maidis (Hemiptera: Cicadellidae) in Brazil?

Machado,

Souza,

Dias

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

BackgroundThe corn leafhopper, Dalbulus maidis, is an important pest in Brazil. While chemical control has traditionally been the cornerstone for managing this pest, field control failures have been reported for some insecticides. To understand if these failures are due to resistance, we evaluated the susceptibility of 11 field‐collected populations of D. maidis to major insecticides during the 2021–2022 crop seasons in Brazil using concentration‐mortality bioassays. Additionally, we employed diagnostic concentration bioassays and foliar sprays at label‐recommended rates in 8–10 populations collected during the 2022–2023 crop seasons.ResultsHigh susceptibility to methomyl, carbosulfan and acephate was observed on concentration‐mortality bioassays across all populations tested with resistance ratio (RR) based on LC50 <10‐fold, except for one population from Bahia State that exhibited reduced susceptibility to methomyl (RR = 17.5). On the other hand, all populations exhibited reduced susceptibility to bifenthrin, acetamiprid, and imidacloprid, with RR ranging from 90 to 2000‐fold. This reduced susceptibility to neonicotinoid and pyrethroid insecticides was further confirmed at diagnostic concentrations based on LC99 of the susceptible strain, with survival rates >20% and in foliar sprays with mortality rates <80%. Most populations exposed to acephate and carbosulfan exhibited low survival rates at diagnostic concentrations (<5%) and high mortality rates in foliar sprays (>80%).ConclusionsThe reduced susceptibility to pyrethroid and neonicotinoid insecticides likely explain the field failures in controlling D. maidis populations in Brazil. This study represents the first large‐scale susceptibility monitoring of D. maidis to insecticides, and the results will contribute to decision‐making regarding the management of this pest. © 2024 Society of Chemical Industry.

show abstract

Field‐evolved resistance to nitenpyram is associated with fitness costs in whitefly

Lu,

Fu,

Tan

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDElucidating fitness cost associated with field‐evolved insect resistance to insecticide is of particular importance to current sustainable pest control. The global pest whitefly Bemisia tabaci has developed resistance to many members of neonicotinoids, but little is known about whitefly resistance to neonicotinoid nitenpyram and its associated fitness cost. Using insecticide bioassay and life‐table approach, this study aims to investigate nitenpyram resistance status in field‐collected whitefly populations, and to explore whether such resistance is accompanied by a fitness cost.RESULTSThe bioassay results revealed that 14 of 29 whitefly populations displayed moderate to extremely high resistance to nitenpyram, demonstrating a widespread field‐evolved resistance to nitenpyram. This field‐evolved resistance in the whitefly has increased gradually over the past 3 years from 2021 to 2023. Further life‐table study showed that two resistant whitefly populations exhibited longer developmental time, shorter lifespans of adult, and lower fecundity compared with the most susceptible population. The relative fitness cost of the two resistant populations was calculated as 0.69 and 0.56 by using net productive rate R0, which suggests that nitenpyram resistance comes with fitness cost in the whitefly, especially on reproduction.CONCLUSIONOverall, these results represent field‐evolved high resistance to nitenpyram in the whitefly. The existing fitness costs associated with nitenpyram resistance are helpful to propose a suitable strategy for sustainable control of whiteflies by rotation or mixture of insecticide with different modes of action. © 2024 Society of Chemical Industry.

show abstract

20E biosynthesis gene CYP306A1 confers resistance to imidacloprid in the nymph stage of Bemisia tabaci by detoxification metabolism

Cited by 10 publications

References 62 publications

Managing Super Pests: Interplay between Pathogens and Symbionts Informs Biocontrol of Whiteflies

Managing Super Pests: Interplay between Pathogens and Symbionts Informs Biocontrol of Whiteflies

Is insecticide resistance a factor contributing to the increasing problems with Dalbulus maidis (Hemiptera: Cicadellidae) in Brazil?

Field‐evolved resistance to nitenpyram is associated with fitness costs in whitefly

Contact Info

Product

Resources

About