<scp>Anti‐Kg</scp> antibodies induce monocyte phagocytosis of the red blood cells

Tanaka, Mitsunobu; Horikawa, Toshihiro; Kusumi, Tomoko; Tateyama, Hidemi; Kimura, Takafumi; Fujimura, Yoshihiro; Hirayama, Fumiya; Tani, Yoshihiko; Takihara, Yoshihiro

doi:10.1111/trf.16651

Cited by 1 publication

(4 citation statements)

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“…Because there is currently no information available regarding the clinical significance of anti‐DSLK antibodies, we performed monocyte phagocytosis assays to determine whether anti‐DSLK antibodies can cause HTR or HDN. The maximum percent phagocytosis of RBCs sensitized with anti‐D and anti‐Kg antibodies was >80%, consistent with the results of a previous study [7], whereas the maximum percent phagocytosis of RBCs sensitized with anti‐DSLK was 64%. In addition, the percent phagocytosis increased as the amount of anti‐DSLK bound on the RBCs increased.…”

Section: Discussionsupporting

confidence: 91%

“…Although cases of individuals harbouring anti‐DSLK or anti‐Kg antibodies are very rare, two cases of HDN caused by anti‐Kg antibodies have been reported [2, 6] to date. Another study suggested that anti‐Kg antibodies can cause haemolytic transfusion reaction (HTR) [7]. Because there is currently no information available regarding the clinical significance of anti‐DSLK antibodies, we performed monocyte phagocytosis assays to determine whether anti‐DSLK antibodies can cause HTR or HDN.…”

Section: Discussionmentioning

confidence: 99%

“…Since RHAG was recognized as the 30th blood group system, consist- (HTR) [7]. Because there is currently no information available regarding the clinical significance of anti-DSLK antibodies, we performed monocyte phagocytosis assays to determine whether anti-DSLK antibodies can cause HTR or HDN.…”

Section: Discussionmentioning

confidence: 99%

“…Two cases of haemolytic disease of the newborn (HDN) caused by anti-Kg antibody and requiring exchange transfusion therapy have been reported, demonstrating the clinical importance of anti-Kg antibodies [2,6]. In addition, a study of anti-Kg antibody-mediated phagocytosis of red blood cells (RBCs) by monocytes suggested that these antibodies can cause haemolytic disease of the fetus and newborn [7]. Because anti-DSLK and anti-Kg antibodies are predicted to recognize the amino acid at the same RhAG position, lysine 164 and glutamine 164, respectively, we also examined alloanti-DSLK antibodies using a monocyte phagocytosis assay to estimate the antibody's clinical significance.…”

Section: Introductionmentioning

confidence: 99%

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DSLK and Kg: Antithetical antigens in the RHAG blood group system, and characterization of anti‐DSLK antibody

et al. 2023

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Background and Objectives The RHAG blood group system contains five antigens: Duclos (RHAG001), Ola (RHAG002), DSLK (RHAG003), Kg (RHAG005) and SHER (RHAG006). Individuals who are DSLK‐negative and Kg‐positive have the same allele RHAG*01.–3, with a single‐nucleotide variation (rs144305805), c.490A>C (p.Lys164Gln), in exon 3 of the RHAG gene. We aimed to confirm whether DSLK and Kg are antithetical antigens. Materials and Methods Blood samples of the original DSLK‐negative proband with anti‐DSLK, her son and another DSLK‐negative individual were examined. The RHAG gene was analysed by polymerase chain reaction and Sanger sequencing. Immunocomplex capture fluorescence assays (ICFAs) and monocyte phagocytosis assays were performed to characterize the anti‐DSLK antibody. Cross‐testing of alloanti‐DSLK and monoclonal anti‐Kg (OSK46) was performed using transduced HEK293 cells by inducing the construct of expression vectors encoding wild‐type RHAG*01 or the variant RHAG*01.–3. Results ICFA using monoclonal anti‐RHAG (LA18.18) revealed that the anti‐DSLK and anti‐Kg antibodies reacted with the wild‐type and variant RhAG (Rh‐associated glycoprotein), respectively. The proband and a DSLK‐negative individual appeared to be homozygous for variant RHAG*01.–3, and the proband's son was typed as RHAG*01/RHAG*01.–3 heterozygote. HEK293 cells with wild‐type RhAG reacted with the anti‐DSLK but not anti‐Kg antibody, whereas HEK293 cells expressing the variant RhAG reacted with the anti‐Kg but not anti‐DSLK antibody. Monocyte phagocytosis assays indicated that 64% of red cells sensitized with anti‐DSLK were phagocytosed by monocytes. Conclusion Our results demonstrate that DSLK and Kg are antithetical antigens in the RHAG blood group system. Anti‐DSLK may be a clinically significant antibody.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%