BMP‐7 attenuates TGF‐β1–induced fibroblast‐like differentiation of rat dermal papilla cells

Abstract

Dermal papilla cells (DPCs) show phenotypic plasticity during wound healing. The multipotency of DPCs is well recognized, but the signaling pathways that regulate the differentiation of these cells into fibroblasts are poorly understood. A preliminary experiment showed that transforming growth factor beta1 (TGF-β1) can induce DPCs to differentiate into fibroblast-like cells, which suggests that DPCs may be a source of wound-healing fibroblasts. Bone morphogenetic protein-7 (BMP-7), a member of the TGF-β superfamily, can prevent and reverse fibrosis by counteracting the TGF-β1-mediated profibrotic effect. To determine whether BMP-7 attenuates the TGF-β1-induced differentiation of DPCs into fibroblasts, we established an in vitro system for DPC differentiation and recorded the gene expression patterns that distinguished DPCs from fibroblasts. The proportion of fibroblast-like cells was significantly enhanced in DPCs treated with TGF-β1, as evidenced by immunocytochemistry, flow cytometry, quantitative real-time reverse transcriptase polymerase chain reaction, and Western blot analysis. BMP-7 and TGF-β1 administration substantially decreased fibroblast-like differentiation, indicating inhibition of TGF-β1-induced differentiation. The antagonistic BMP-7- and TGF-β1-activated signaling pathways can be used to promote wound healing or suppress hypertrophic scarring.

TGF-β signaling inhibits canonical BMP signaling pathway during palate development

“…In the present investigation, we observed that canonical TGF-β signaling suppresses canonical BMP signaling activity in the mesenchymal cells of the posterior palate by competing limited Smad4. The antagonism between TGF-β and BMP signaling has also been reported in several other cells, such as dermal papilla cells, cardiomyocytes and breast cancer cells (Bin et al 2013; Grönroos et al 2012; Wang et al 2012). …”

Cryptomphalus aspersa Mollusc Egg Extract Promotes Regenerative Effects in Human Dermal Papilla Stem Cells

“…Additionally, the e-CAF compound appears to increase the capacity of myofibroblast replacement, from the reservoir of mesenchymal stem cells that constitute the hair follicle, acquiring a more fibroblast-like phenotype, as it is known to occur in the presence of TGF-β 1 [47,48]; this effect is also enhanced by the employed substrate. In addition, preliminary work on its ability to induce keratinocyte migration and training of ECM was also shown.…”

Baicalein attenuates hypertrophic scar formation via inhibition of the transforming growth factor‐β/Smad2/3 signalling pathway

“…As a result, selective affinity of a therapeutic agent for its target kinase is necessary to achieve satisfactory outcomes and avoid side‐effects. For example, in terms of scar formation, the bone morphogenetic protein signalling pathway, which is mediated by other ALKs, has been shown recently to play an antagonistic role against fibroplasia . The ideal anti‐HPS drug should preferably have an affinity with ALK5.…”