BSAand Fibrinogen Adsorption on Chitosan/κ‐Carrageenan Polyelectrolyte Complexes

Abstract

PECs of chitosan/κ-carrageenan are prepared in three different volumetric rations. The complex formation is characterized in order to evaluate the blending formation. Blood compatibility is evaluated by protein adsorption (BSA and fibrinogen) and PEC toxicities are determined with fibroblast cell viability and proliferation. The swelling degree of PECs decreases when the amount of chitosan increases. Due to the linked film formation, PECs decrease BSA adsorption and increase fibrinogen adsorption when compared to the pristine chitosan and κ-carrageenan films. Although pristine chitosan and κ-carrageenan films produced similar cell expansion and viability, the PEC 50:50 vol% chitosan/κ-carrageenan PEC may be acceptable as a new scaffold for cell therapies, due to their effect on cell survival.

Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCsin vitro

“…In this study, fetal bovine serum (FBS) containing more than 10,000 proteins (mainly albumin and immunoglobulin) was used to assess the protein adsorption capacity of our prepared scaffolds. As shown in Figure B, the CCG scaffolds absorbed more proteins than that of the CG scaffolds, which is due to the larger surface area of CCG scaffolds caused by the smaller pore size . In addition, ι‐CA is negatively charged under the physiological pH condition, creating an electrochemical environment favorable for the absorption of immunoglobulin (IgG) with the pI ranging from 7.4 to 8.6 .…”

In vitro evaluation of anti-calcification and anti-coagulation on sulfonated chitosan and carrageenan surfaces

“…Carneiro et al [12] showed that increasing the amount of chitosan in the composition leads to a higher interaction of chitosan (attractive effect) than for carrageenan (repulsive effect) with plasma proteins. Xa.…”

“…The carrageenan solution was prepared by dissolving of 2 g of polymer in 100 mL of 0.5M KCl solution, according to the methodology proposed by Carneiro et al [12]. The PEC formation is considerably influenced by the interaction between the polyelectrolyte and metal ions.…”

“…Carrageenan can be utilized in various forms as beads [9,10], films [11,12], and polyelectrolyte complexes (PEC) [12,13]. Figure 1C shows the κ-carrageenan structure.…”

“…Results showed that, when the chitosan was modified, a lower bovine serum albumin (BSA) and fibrinogen adsorptions were observed. Carneiro et al [12] also evaluated the BSA and fibrinogen adsorption on chitosan/κ-carrageenan polyelectrolyte complexes (PECs). The PECs decreased the BSA adsorption and increased the fibrinogen adsorption, when compared to pristine and κ-carrageenan films.…”