Cotton AnnGh3 Encoding an Annexin Protein is Preferentially Expressed in Fibers and Promotes Initiation and Elongation of Leaf Trichomes in Transgenic Arabidopsis

Abstract

The annexins are a multifamily of calcium-regulated phospholipid-binding proteins. To investigate the roles of annexins in fiber development, four genes encoding putative annexin proteins were isolated from cotton (Gossypium hirsutum) and designated AnnGh3, AnnGh4, AnnGh5, and AnnGh6. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results indicated that AnnGh3, AnnGh4, and AnnGh5 were preferentially expressed in fibers, while the transcripts of AnnGh6 were predominantly accumulated in roots. During fiber development, the transcripts of AnnGh3/4/5 genes were mainly accumulated in rapidly elongating fibers. With fiber cells further developed, their expression activity was dramatically declined to a relatively low level. In situ hybridization results indicated that AnnGh3 and AnnGh5 were expressed in initiating fiber cells (0-2 DPA). Additionally, their expression in fibers was also regulated by phytohormones and [Ca(2+)]. Subcellular localization analysis discovered that AnnGh3 protein was localized in the cytoplasm. Overexpression of AnnGh3 in Arabidopsis resulted in a significant increase in trichome density and length on leaves of the transgenic plants, suggesting that AnnGh3 may be involved in fiber cell initiation and elongation of cotton.

RETRACTED ARTICLE: Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton

“…In this study, we found that GhAnn1 is preferentially expressed both in vegetative and reproductive tissues (Fig. 4), which is different from the cotton annexins reported previously that are expressed and function preferentially in cotton fibers (Huang et al 2013;Li et al 2013). Further expression analysis showed that GhAnn1 is upregulated by diverse abiotic stresses and phytohormones (Fig.…”

“…3), whether expressed in onion in vivo or tobacco in vitro. The location is differs from AnnGh3, which is found in the cytoplasm (Li et al 2013), and AnxGb6, which localizes to the plasma membrane and nucleolus (Huang et al 2013). The plasma membrane is a key interface between plant cells and their environment, which play key roles in mediating responses to the endogenous and environmental cues that regulate various developmental and metabolic events (Barbara and Christian 2013).…”

“…Expression of four genes encoding putative annexin proteins (AnnGh3-AnnGh6) is regulated during fiber development and induced by some plant hormones and Ca 2+ . Overexpression of AnnGh3 in Arabidopsis resulted in a significant increase in leaf trichome density and length (Li et al 2013).…”

A Cotton Annexin Affects Fiber Elongation and Secondary Cell Wall Biosynthesis Associated with Ca2+ Influx, ROS Homeostasis, and Actin Filament Reorganization

“…Transgenic cotton seedlings overexpressing AnnBj1 and GhAnn1 both showed welldeveloped root systems with significantly enhanced root length under salt and drought stress (Divya et al, 2010;Zhang et al, 2015a). AnnGh3, which is preferentially expressed in fibers, promotes the initiation and elongation of leaf trichomes (Li et al, 2013b). Ectopic AnxGb6 in Arabidopsis enhanced root elongation, and more F-actin accumulated in the basal part of the root cell elongation zone.…”

Cloning, expression, and in silico analysis of a novel annexin gene FtANX1from Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.)

“…Research in strawberry indicates that Annfaf may be involved in the ripening progress of strawberry fruit (Chen et al, 2016). AnnGh5 has been described to be preferentially expressed in fibers and is mainly accumulated in rapidly elongating fibers (Li et al, 2013). As FtANX1 is grouped into Group II together with AnnMt1, Annfaf, and AnnGh5, we suspect that FtANX1 might have similar functions and be involved in cell signaling and ripening processes.…”