<scp>C1</scp>Factor

Vogel, Joseph P.; Kristie, Thomas M.

doi:10.1002/0471203076.emm1031

Cited by 1 publication

(1 citation statement)

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“…The enhancer core elements are the primary regulatory units and are sites for the assembly of protein complexes containing the POU homeodomain protein Oct-1, the viral transactivator protein VP16 [infected cell protein (ICP) 25, ␣-trans-induction factor], and the coactivator host cell factor 1 (HCF-1; C1) (1)(2)(3)(4). The IE genes have been a model system for delineating mechanisms of combinatorial control and multiprotein transcription assemblies.…”

Section: T He Transcriptional Regulation Of the Immediate Early (Ie)mentioning

confidence: 99%

Herpes simplex virus infections are arrested in Oct-1-deficient cells

Nogueira

Wang

Tantin

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Expression of the herpes simplex virus (HSV) immediate early (IE)genes is regulated by a multiprotein complex that is assembled on the TAATGARAT enhancer core element. The complex contains the cellular POU domain protein Oct-1, the viral transactivator VP16, and the cellular cofactor host cell factor 1. The current model suggests that the assembly depends on recognition of the core element by Oct-1. Here, HSV infection of Oct-1-deficient mouse embryonic fibroblast cells demonstrates that Oct-1 is critical for IE gene expression at low multiplicities of infection (moi). However, the protein is not essential for IE gene expression at high moi, indicating that VP16-mediated transcriptional induction through other IE regulatory elements is also important. This induction depends, at least in part, on the GA-binding protein binding elements that are present in each IE enhancer domain. Surprisingly, whereas the viral IE genes are expressed after high moi infection of Oct-1-deficient cells, the assembly of viral replication factories is severely impaired, revealing a second critical role for Oct-1 in HSV replication. The results have implications for both the HSV lytic and latency-reactivation cycles.

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Section: T He Transcriptional Regulation Of the Immediate Early (Ie)mentioning

confidence: 99%