“…Several methods have been used to evaluate RCS, which could assess the risk of tearing and perforation at the furcation area, such as histological sections 9 , serial sections 10 , clearing 11 , scanning electron microscopy 12 , stereomicroscope 13 , radiographs 14 , silicone impressions of the root canal 15 , CBCT 16,17 , and micro computed tomography (micro--CT) 18 . Among clinical methods, which can be used in vivo, CBCT is a promising one, since it allows tridimensional evaluation 19,20 . For in vitro studies, both micro-CT and stereomicroscope images are used for gold standard references in RCS evaluations [21][22][23] .…”