<scp>CD</scp>147 promotes progression of head and neck squamous cell carcinoma via <scp>NF</scp>‐kappa B signaling

Yu, Bin; Zhang, Yan; Wu, Kailong; Wang, Lizhen; Jiang, Yingying; Chen, Wantao; Yan, Ming

doi:10.1111/jcmm.13996

Cited by 31 publications

(30 citation statements)

References 36 publications

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“…Nevertheless, on the basis of these findings, we further explored the possible mechanisms underlying the effect of CD147 on the development and recurrence of OSCC, especially cervical lymph node metastasis. EMT was reported to be closely related to lymph node or distant metastasis in some malignant tumours, especially squamous cell carcinomas 6,28,29 . We found that E‐cadherin expression in the OSCC tissues was significantly lower than that in the adjacent oral mucosa.…”

Section: Discussionmentioning

confidence: 61%

CD147 promotes proliferation and migration of oral cancer cells by inhibiting junctions between E‐cadherin and β‐catenin

Min

Xiong

Wang

et al. 2020

J Oral Pathology Medicine

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Background Although association between oral squamous cell carcinoma (OSCC) with epithelial‐mesenchymal transition (EMT) has been demonstrated, we found CD147, one transmembrane protein we previously studied in oral submucous fibrosis, was correlated with E‐cadherin, one marker of EMT. Here, we investigated CD147 expression in the different stages of OSCC and assessed its association with epithelial‐mesenchymal transition (EMT). Materials and methods CD147 and E‐cadherin expression in tissue microarrays containing 48 OSCC specimens and matched adjacent tissues was analysed using immunohistochemistry. CD147 was overexpressed or knocked down using exogenous cloning vector and RNA interference, respectively, in OSCC cell lines. Cell proliferation and migration were measured using the CCK8 assay and scratch test, respectively. The expression and localization of EMT‐associated proteins was analysed by Western blotting and immunofluorescence. Results CD147 expression in OSCC tissues was significantly higher than that in adjacent tissues and was markedly higher in cancer tissues with metastasis (P < .05). CD147 expression showed significant negative correlation with E‐cadherin expression. CD147 overexpression downregulated E‐cadherin and inhibited its complex with β‐catenin and then upregulated N‐cadherin and vimentin. Additionally, alterations in CD147 protein expression affected proliferation and migration ability in OSCC cells and were related to β‐catenin nuclear translocation. Conclusion CD147 plays an important role in tumorigenesis and metastasis by promoting EMT progression in OSCC. It may be considered as a novel potential diagnostic and therapeutic target for OSCC.

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Section: Discussionmentioning

confidence: 61%

CD147 promotes proliferation and migration of oral cancer cells by inhibiting junctions between E‐cadherin and β‐catenin

Min

Xiong

Wang

et al. 2020

J Oral Pathology Medicine

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“…We discovered that the SDGs were mainly enriched in the NF−kappaB signaling pathway and DNA repair by GO and KEGG analyses. Several studies have strongly supported the associations between NF−kappaB signaling pathway and HNC [20][21][22] . Qin Y et.al found the CCL18 (chemokine (C-C motif) ligand 18) could promote the HNSCC malignancy and its level was signi cantly associated with histological grade through regulating the NF−κB signal pathway [20] .…”

Section: Discussionmentioning

confidence: 94%

Identification and Clinical Validation of Genes Signatures With Grade and Survival in Head and Neck Carcinomas

Cao

She

2020

Preprint

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Background: The mechanism of transition from low-grade to high-grade head and neck carcinomas (HNC) still remains unclear. The aim of this study was to explore the genes expression profiles that drive malignancy from low to high-grade HNC, as well as analyze their correlations with the survival.Methods: Gene expressions and clinical data of HNC were downloaded from the Gene Expression Omnibus (GEO) repository. The significantly differential genes (SDGs) between low and high-grade HNC were screened by GEO2R and R software. Bioinformatics functions of SDGs were investigated by the enrichment analyses. Univariate and multivariate cox regressions were performed to identify prognostic SDGs of progression free survival (PFS) and disease specific survival (DSS). ROC curve was established to evaluate the ability to predict the prognosis. Then, the correlations between SDGs and clinical features were evaluated. The genes were experimentally validated by RT-PCR in clinical specimens’ tissues at last.Results: Thirty-five SDGs were identified in 47 low-grade and 30 high-grade HNC samples. Enrichment analysis showed these SDGs were mainly enriched in the DNA repair pathway and the regulation of I−kappaB kinase/NF−kappaB signaling pathway. Cox regression analyses showed that CXCL14, SLC44A1 and UBD were significantly associated with DSS, and PPP2R2C and SLC44A1 were associated with PFS. Patients at a high-risk or low-risk for prognosis were established based on genes signatures. High-risk patients had significantly shorter DSS and PFS than low-risk patients (P=0.033, 0.010 respectively). Multivariate cox regression showed HPV (P=0.033), lymph node status (P=0.032) and residual status (P＜0.044) were independent risk factors for PFS. ROC curves showed the risk score had better efficacy to predict survival both for DSS and PFS (AUC=0.858, 0.901 respectively). In addition, we found UBD, PPP2R2C and risk score were significantly associated with HPV status (all P＜0.05). The experiment results showed CXCL14 and SLC44A1 were significantly overexpressed in the HNC grade I/II tissues and the UBD were overexpressed in the HNC grade III/IV tissues.Conclusions: Our results suggested that SDGs had different expression profiles between the low-grade and high-grade HNC, and these genes may serve as prognostic biomarker to predict the survival.

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“…We discovered that the SDGs were mainly enriched in the NF − kappaB signaling pathway and DNA repair by GO and KEGG analyses. Several studies have strongly supported the associations between NF − kappaB signaling pathway and HNC [20][21][22] . Qin Y et.al found that CCL18 (chemokine (C-C motif) ligand 18) could promote the HNSCC malignancy and its level was signi cantly associated with histological grade through regulating the NF − κB signal pathway [20] .…”

Section: Discussionmentioning

confidence: 94%

Identification and Clinical Validation of Genes Signatures with Grade and Survival in Head and Neck Carcinomas

Cao

She

2020

Preprint

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Background: The mechanism of transition from low-grade to high-grade head and neck carcinomas (HNC) still remains unclear. This study aimed to explore the genes expression profiles that drive malignancy from low to high-grade HNC, as well as analyze their correlations with survival.Methods: Gene expressions and clinical data of HNC were downloaded from the Gene Expression Omnibus (GEO) repository. The significantly different genes (SDGs) between low and high-grade HNC were screened by GEO2R tool and R software. Bioinformatics functions of SDGs were investigated by the enrichment analyses. Univariate and multivariate cox regressions were performed to identify prognostic SDGs of progression-free survival (PFS) and disease-specific survival (DSS). Receiver operating curve (ROC) was established to evaluate the ability to predict the prognosis. Then, the correlations between SDGs and clinical features were evaluated. The genes were experimentally validated by RT-PCR in clinical specimens.Results: 35 SDGs were identified in 47 low-grade and 30 high-grade HNC samples. Enrichment analysis showed these SDGs were enriched in the DNA repair pathway and the regulation of I−kappaB kinase/NF−kappaB signaling pathway. Cox regression analyses showed that CXCL14, SLC44A1 and UBD were significantly associated with DSS, and PPP2R2C and SLC44A1 were associated with PFS. Patients at a high-risk or low-risk for prognosis were established based on genes signatures. High-risk patients had significantly shorter DSS and PFS than low-risk patients (P=0.033, 0.010 respectively). Multivariate cox regression showed human papillomavirus (HPV) (P=0.033), lymph node status (P=0.032) and residual status (P＜0.044) were independent risk factors for PFS. ROC curves showed the risk score had better efficacy to predict survival both for DSS and PFS (AUC=0.858, 0.901 respectively). In addition, we found UBD, PPP2R2C and risk score were significantly associated with HPV status (all P＜0.05). The experiment results showed CXCL14 and SLC44A1 were significantly overexpressed in the HNC grade I/II tissues and the UBD was overexpressed in the HNC grade III/IV tissues.Conclusions: Our results suggested that SDGs had different expression profiles between the low-grade and high-grade HNC, and these genes may serve as prognostic biomarker to predict the survival.

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CD147 promotes progression of head and neck squamous cell carcinoma via NF‐kappa B signaling

Cited by 31 publications

References 36 publications

CD147 promotes proliferation and migration of oral cancer cells by inhibiting junctions between E‐cadherin and β‐catenin

CD147 promotes proliferation and migration of oral cancer cells by inhibiting junctions between E‐cadherin and β‐catenin

Identification and Clinical Validation of Genes Signatures With Grade and Survival in Head and Neck Carcinomas

Identification and Clinical Validation of Genes Signatures with Grade and Survival in Head and Neck Carcinomas

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