2016
DOI: 10.1111/bjh.13941
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CD45RA, a specific marker for leukaemia stem cell sub‐populations in acute myeloid leukaemia

Abstract: Chemotherapy resistant leukaemic stem cells (LSC) are thought to be responsible for relapses after therapy in acute myeloid leukaemia (AML). Flow cytometry can discriminate CD34(+) CD38(-) LSC and normal haematopoietic stem cells (HSC) by using aberrant expression of markers and scatter properties. However, not all LSC can be identified using currently available markers, so new markers are needed. CD45RA is expressed on leukaemic cells in the majority of AML patients. We investigated the potency of CD45RA to s… Show more

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Cited by 57 publications
(44 citation statements)
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“…We then investigated CD9 expression on normal hematopoietic progenitors (HSCs) and on their leukemic counterparts (LSCs) to use CD9 as a marker of MRD in the most immature compartment. We studied progenitor cell populations based on the expression of the following antigens: HSC (CD34+CD38−CD45RA−CD90+), Multipotent Progenitors (MPP: CD34+CD38−CD45RA−CD90dim), Lymphoid‐Primed Multipotent Progenitors (LMPP: CD34+CD38−CD45RA+CD90−), and the putative LSCs (CD34+CD38−) which harbored various patterns of expression of CD90 and CD45RA . These phenotypic definitions of progenitor cells have been previously verified in functional assays …”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…We then investigated CD9 expression on normal hematopoietic progenitors (HSCs) and on their leukemic counterparts (LSCs) to use CD9 as a marker of MRD in the most immature compartment. We studied progenitor cell populations based on the expression of the following antigens: HSC (CD34+CD38−CD45RA−CD90+), Multipotent Progenitors (MPP: CD34+CD38−CD45RA−CD90dim), Lymphoid‐Primed Multipotent Progenitors (LMPP: CD34+CD38−CD45RA+CD90−), and the putative LSCs (CD34+CD38−) which harbored various patterns of expression of CD90 and CD45RA . These phenotypic definitions of progenitor cells have been previously verified in functional assays …”
Section: Resultsmentioning
confidence: 99%
“…Over the past few years, various markers have been described to better characterize LSC or blast cells from AML with normal cytogenetics (CN‐AML) for minimal residual disease (MRD) monitoring . Furthermore, targeting MRD by multiparametric flow cytometry (MFC) to monitor treatment efficiency is one of the greatest challenges in the treatment of AML.…”
Section: Introductionmentioning
confidence: 99%
“…Using such specific leukemia associated LSC immunophenotypes enables detection of LSCs in the vast majority of CD34 positive AML cases (Hanekamp, Cloos, & Schuurhuis, ). LSCs, as defined as aberrant marker positive CD34+CD38− cells, harbor specific molecular aberrancies (e.g., FLT3‐ITD, NPM1 mut ) and thus are indeed leukemic cells (Kersten et al., ; Schuurhuis et al., ; Terwijn et al., ).…”
Section: Commentarymentioning
confidence: 99%
“…Both CD44 and GPR56 were expressed on >90% of CD34 + 38 − HSPCs, while the markers CD117, CD305, CD47 and CD33 were prevalent in substantial numbers of normal HSPCs with a surface expression on 50%-90% of cells, comparably to what has been reported. [21][22][23][24][25][26][27][28] The remaining markers were hardly expressed on CD34 + 38 − HSPCs ( Figure 1B).…”
Section: Mfc Analysis Of Cell Surface Markers For Residual Leukemicmentioning
confidence: 99%
“…Since no single marker showed an ideal expression profile for leukemic cell enrichment, we aimed to determine the best marker combination enabling enrichment of residual leukemic cells in a maximum number of AML cases. Although CD47, GPR56, CD33, CD305 and CD45RA were expressed on >90% blasts of the majority of AML cases evaluated, these markers were not considered further for enrichment, because they were also widely expressed on normal hematopoietic cell subtypes including CD34 + 38 − HSPCs [21][22][23][24][25] ( Figure 1B and Figure S2). From that perspective CLL-1, TIM-3, CD117 and CD123 were the most promising markers, since they were only expressed on either a distinct progenitor population or on small subtypes of mature leukocytes.…”
Section: Mfc Analysis Of Cell Surface Markers For Residual Leukemicmentioning
confidence: 99%