CE with chemiluminescence detection for the determination of thyroxine in human serum

Abstract

A sensitive and rapid approach to perform thyroxine (T4) assay by CE with chemiluminescence (CL) detection was developed. The sensitive detection was based on the enhancement effect of T4 on the CL reaction between luminol and potassium permanganate (KMnO4 ) in alkaline solution. A laboratory-built reaction flow cell and a photon counter were deployed for the CL detection. Experimental conditions for CL detection were studied in detail to achieve maximum assay sensitivity. Optimal conditions were found to be 5.0 × 10(-4) M luminol added to the CE running buffer and 9.2 × 10(-5) M KMnO4 in 0.072 M NaOH solution introduced postcolumn. In the optimized experimental conditions, the linear range for T4 detection was 6.0 × 10(-8) -6.0 × 10(-6) M, with the detection limit of 2.0 × 10(-8) M (S/N = 3). Six human serum samples from healthy subjects, hyperthyroid patients and hypothyroid patients were analyzed by the presented method. The serum level of T4 in healthy subjects was found be 9.0 × 10(-8) M, whereas the T4 level was found to be 15.6 × 10(-8) M in hyperthyroid patients and 1.3 × 10(-8) M in hypothyroid patients. The results suggested a potential application of the proposed assay in rapid primary diagnosis of diseases such as hyperthyroid and hypothyroid.

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

“…Li et al. employed CE‐UV to measure six thyroid hormones in human serum samples following preanalysis treatment of the serum by ion pair hollow fiber liquid‐liquid‐liquid micro‐extraction. Employing this technique the authors could separate diiodothyronine, 3,3,5‐triiodo‐l‐thyronine, 3,5,3,5‐tetraiodolthyronine, 3,3,5‐triiodothyronine, monoiodotyrosine, and diiodotyrosine in 13 min using 20 kV and a BGE of 25 mM phosphate buffer, pH 2.5 plus 10% v/v acetonitrile and 0.5% m/v polyethylene glycol.…”

Recent advances in chemiluminescence detection coupled with capillary electrophoresis and microchip capillary electrophoresis

“…Mu et al. also described a sensitive and rapid approach to perform thyroxine assay by CE–CL detection, which was based on the enhancement effect of thyroxine on the CL reaction between luminol and potassium permanganate in alkaline solution. A lab‐built reaction flow cell and a photon counter were deployed for the CL detection.…”

“…In some cases, the analytes were labeled by CL reagents such as N ‐(4‐aminobutyl)‐ N ‐ethylisoluminol (ABEI) for CE–CL detection. Table shows drug analysis by CE–CL system in recent decade.…”

Thyroxine Quantification by Using Plasmonic Nanoparticles as SERS Substrates

“…Literature can be found that reports on thyroid hormones (a small molecule type) in human or animal serum, urine, or tissues, either in its pure or synthetic form, by using sensors or methods that are the outcome of combining more than two techniques and at least one kind of antibody, all of them with the aim of contributing to the understanding of hypo-and hyperthyroidism. In that sense, a list of techniques includes chromatography coupled with mass spectroscopy [10][11][12][13][14][15], immunoassays with magnetic or plasmonic nanoparticles [16][17][18], electrochemical or chemiluminescence techniques complemented with immunoassays and/or nanoparticles [19][20][21][22][23][24], among others. Some of those techniques require pretreatment, purification, or labeling of the sample, while others use high-pressure conditions, which generate an increase in the analysis costs [10,12,13,16].…”