CircHOMER1 inhibits porcine adipogenesis via the miR‐23b/SIRT1 axis

Abstract

Fat deposition is critical to pork quality. However, the mechanism of fat deposition remains to be elucidated. Circular RNAs (circRNAs) are ideal biomarkers and are involved in adipogenesis. Here, we investigated the effect and mechanism of circHOMER1 on porcine adipogenesis in vitro and in vivo. Western blotting, Oil red O staining, and HE staining were used to assess the function of circHOMER1 in adipogenesis. The results showed that circHOMER1 inhibited adipogenic differentiation of porcine preadipocytes and suppressed adipogenesis in mice. Dual-luciferase reporter gene, RIP, and pull-down assays demonstrated that miR-23b directly bound to circHOMER1 and the 3′-UTR of SIRT1. Rescue experiments further illustrated the regulatory relationship among circHOMER1, miR-23b, and SIRT1. Conclusively, we demonstrate that circHOMER1 plays an inhibitory role in porcine adipogenesis through miR-23b and SIRT1. The present study revealed the mechanism of porcine adipogenesis, which may be helpful to improve pork quality.

CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/MEF2C axis

“… 43 Furthermore, previous studies conducted have indicated that circHOMER1 may inhibit adipogenic differentiation in porcine preadipocytes and C3H10T1/2 cells, thereby suppressing adipogenesis in mice through its interaction with SIRT1 via miR-23b. 44 , 45 This current study provides further confirmation that circMEF2C(2, 3) actively participates in the regulation of adipocyte proliferation and adipogenesis by functioning as a miR-383 and miR-671-3p sponge.…”

CircRNA-5335 Regulates the Differentiation and Proliferation of Sheep Preadipocyte via the miR-125a-3p/STAT3 Pathway

“…For example, the overexpression of miR-125a-5p promotes the proliferation but inhibits the differentiation of preadipocytes in pigs [ 17 ], and miR-125a-3p may regulate preadipocyte differentiation by targeting FSTL1 [ 18 ]. Furthermore, previous research has investigated the effect of circHOMER1 on porcine adipogenesis ex vivo and in vivo, demonstrating that circHOMER1 performed a depressant effect in adipogenesis by SIRT1 and miR-23b [ 19 ].…”