<scp>CPT</scp>1A‐mediated succinylation of S100A10 increases human gastric cancer invasion

Wang, Chao; Zhang, Chen; Li, Xiang; Shen, Jiajia; Xu, Yue; Shi, Hui; Mu, Xiaoli; Pan, Jinshun; Zhao, Ting; Li, Mengjing; Geng, Biao; Xu, Chenjie; Wen, Hao; Qin, You

doi:10.1111/jcmm.13920

Cited by 88 publications

(96 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, S100A10 and annexin A2 can be used as candidate prognostic biomarkers of epithelial ovarian cancer 30 ; CPT1A-mediated S100A10 succinylation promotes the progression of GC. 25 Herein, we found that S100A10 expression was increased in HCC. Furthermore, our luciferase activity experiment proved that miR-320 could target S100A10.…”

Section: Discussionmentioning

confidence: 73%

“…GAPDH, glyceraldehyde 3-phosphate dehydrogenase; miR-320, microRNA-320; NC, nitrocellulose therapy targets for HCC. 23 Serum miRNA-27a and miRNA-18b can serve as potential predictive biomarkers for hepatitis C-related HCC 24 ; the methylation of miR-183 is closely linked to a poor survival of HCC patients 25 ; miRNA-211 inhibits cell proliferation, migration and invasion by targeting SPARC in human HCC. 26 Similarly, this study found that miR-320 expression in HCC tissues was decreased than normal liver epithelial tissues.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR‐320/S100A10 axis

Zhao

Chi

Sun

et al. 2020

Cell Biochemistry & Function

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is one of the deadliest cancers. Multiple long non-coding RNAs (lncRNAs) are recently identified as crucial oncogenic factors or tumour suppressors. In this study, we explored the effects of LINC00174 on the progression of HCC. Expression levels of LINC00174 and microRNA-320 (miR-320) in HCC tissue samples were measured using quantitative real-time polymerase chain reaction (qRT-PCR). The association between pathological indices and LINC00174 was also analysed. Human HCC cell lines Hep3B and Huh7 were used as cell models. CCK-8 and bromodeoxyuridine (BrdU) assays were used to assess the effect of LINC00174 on HCC cell line proliferation. Flow cytometry was used to study the effect of LINC00174 on HCC apoptosis. Transwell assay was conducted to detect the effect of LINC00174 on migration and invasion. Furthermore, luciferase reporter assay and RNA immunoprecipitation (RIP) assay were used to confirm the binding relationship between miR-320 and LINC00174. Additionally, western blot was used to detect the regulatory function of LINC00174 on oncogene S100 calcium binding protein A10 (S100A10). We demonstrated that LINC00174 expression in HCC clinical samples was significantly increased and this was correlated with higher T stage. Its overexpression remarkably accelerated proliferation and metastasis of HCC cells while reduced apoptosis. Accordingly, knockdown of it suppressed the malignant phenotypes of HCC cells. Overexpression of LINC00174 significantly reduced the expression of miR-320 by sponging it, in turn enhanced the expression of S100A10. In conclusion, LINC00174 is a sponge of tumour suppressor miR-320, enhances the expression of S100A10 indirectly and functions as an oncogenic lncRNA in HCC. Significance of the study: LINC00174 is a novel lncRNA, whose function is rarely investigated. It is reported that it is oncogenic in colorectal cancer, while its role in HCC remains unclear. Herein, we report that LINC00174 is significantly up-regulated in HCC tissues and promotes the malignant phenotypes. We demonstrate that LINC00174 functions as a sponge for miR-320, increases the expression level of oncogene S100A10 in HCC. This study helps clarify the mechanism of HCC tumorigenesis and progression, and uncover the role of LINC00174 in human disease.

show abstract

Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR‐320/S100A10 axis

Zhao

Chi

Sun

et al. 2020

Cell Biochemistry & Function

View full text Add to dashboard Cite

show abstract

“…Desuccinylation by SIRT5 suppresses the activities of pyruvate dehydrogenase complex and succinate dehydrogenase (117) leading to the accumulation of succinate and mitochondrial reactive oxygen species, thereby activating HIF1α (122). SIRT5 can also cause desuccinylation and negative regulation of S100A10, a protein that regulate invasion and motility (123). Generally, NAD + levels influence the secretion of various cytokines by inflammatory cells (124).…”

Section: Sirtuins In Tumor Angiogenesis and Their Regulatory Strategiesmentioning

confidence: 99%

Role of Sirtuins in Tumor Angiogenesis

et al. 2020

View full text Add to dashboard Cite

Generally, changes in the metabolic status of cells under conditions like hypoxia and accumulation of lactate can be sensed by various sensing mechanisms, leading to modulation of a number of signal transduction pathways and transcription factors. Several of the proangiogenic cytokines like VEGF, FGF, PDGF, TGF-β, Ang-2, ILs, etc. are secreted by cancer cells, under hypoxic microenvironment. These cytokines bind to their receptors on the endothelial cells and activates a number of signaling pathways including Akt/PIP3, Src, p38/MAPK, Smad2/3, etc., which ultimately results in the proliferation and migration of endothelial cells. Transcription factors that are activated in response to the metabolic status of tumors include HIFs, NF-κb, p53, El-2, and FOXO. Many of these transcription factors has been reported to be regulated by a class of histone deacetylase called sirtuins. Sirtuins are NAD + dependent histone deacetylases that play pivotal role in the regulation of tumor cell metabolism, proliferation, migration and angiogenesis. The major function of sirtuins include, deacetylation of histones as well as some non-histone proteins like NF-κB, FOXOs, PPAR , PGC1-α, enzymes like acetyl coenzymeA and structural proteins like α tubulin. In the cell, sirtuins are generally considered as the redox sensors and their activities are dependent on the metabolic status of the cell. Understanding the intricate regulatory mechanisms adopted by sirtuins, is crucial in devising effective therapeutic strategies against angiogenesis, metastasis and tumor progression. Keeping this in mind, the present review focuses on the role of sirtuins in the process of tumor angiogenesis and the regulatory mechanisms employed by them.

show abstract

“…Succinylated S100A10 was stabilized at the protein level by the suppression of its ubiquitylation. Moreover, carnitine palmitoyltransferase 1A (CPT1A) was found to function as a lysine succinyltransferase that regulates S100A10 succinylation . SIRT5 is a well‐known enzyme that catalyzes lysine desuccinylation in mammalian cells, as supported by the findings that SIRT5‐KO mice exhibit potently increased global lysine succinylation levels .…”

Section: The Role Of Acylation In Cancer Progressionmentioning

confidence: 99%

Advances of Proteomics in Novel PTM Discovery: Applications in Cancer Therapy

Wang

Zhang

et al. 2019

Small Methods

View full text Add to dashboard Cite

Protein posttranslational modification (PTM) is a critical mechanism to enhance the diversity of protein species and functions in organisms. Currently, many different PTMs are discovered and found to be involved in a wide range of cellular processes. The aberrant regulation of PTM dynamics is reported to contribute to cancer development. Phosphorylation and acetylation are the best studied PTMs, and the application of high‐resolution mass spectrometry‐based proteomics and specific antibody‐based enrichment technologies significantly promotes novel PTM discovery. This review discusses methods for global PTM identification, including antibody‐based enrichment and chemical probe‐based identification. Several acylations, such as propionylation, butyrylation, succinylation, malonylation, and crotonylation, are recently found to influence both histone and nonhistone proteins and to be intimately linked with the progression of cancer. It is proposed that targeting novel PTM dynamics by pharmacological inhibitors has great potential for cancer therapy.

show abstract

CPT1A‐mediated succinylation of S100A10 increases human gastric cancer invasion

Cited by 88 publications

References 46 publications

LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR‐320/S100A10 axis

LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR‐320/S100A10 axis

Role of Sirtuins in Tumor Angiogenesis

Advances of Proteomics in Novel PTM Discovery: Applications in Cancer Therapy

Contact Info

Product

Resources

About