2022
DOI: 10.1111/pbi.13872
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CRISPR RNA‐guided integrase enables high‐efficiency targeted genome engineering in Agrobacterium tumefaciens

Abstract: Summary Agrobacterium tumefaciens, the causal agent of plant crown gall disease, has been widely used to genetically transform many plant species. The inter‐kingdom gene transfer capability made Agrobacterium an essential tool and model system to study the mechanism of exporting and integrating a segment of bacterial DNA into the plant genome. However, many biological processes such as Agrobacterium‐host recognition and interaction are still elusive. To accelerate the understanding of this important plant path… Show more

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Cited by 19 publications
(20 citation statements)
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“…Plant genetic transformation is very important for basic biology research and modern plant breeding. At present, the Agrobacterium -mediated genetic transformation method is a widely used transformation technology [ 1 ]. Agrobacterium tumefaciens is a gram-negative soil bacterium that contains the Ti plasmid.…”
Section: Discussionmentioning
confidence: 99%
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“…Plant genetic transformation is very important for basic biology research and modern plant breeding. At present, the Agrobacterium -mediated genetic transformation method is a widely used transformation technology [ 1 ]. Agrobacterium tumefaciens is a gram-negative soil bacterium that contains the Ti plasmid.…”
Section: Discussionmentioning
confidence: 99%
“…Plant genetic transformation is a key step in molecular design breeding, including genetic engineering and genome editing [ 1 ]. It can not only be used to identify gene functions but also to improve short plate traits of plant varieties, especially for genetic improvement of target traits of tree varieties with a long breeding generation cycle, which can significantly shorten the breeding cycle [ 2 ].…”
Section: Introductionmentioning
confidence: 99%
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“…These modifications are either in CRISPR vector systems, explant selection, media composition, or the addition of some regulators [ 35 , 36 , 38 , 39 ]. Recently, to enhance the efficiency of Agrobacterium -mediated transformation for CRSIPR-Cas9, scientists have targeted the Agrobacterium genome [ 40 , 41 ] however, the technology faces multiple challenges that need to be improved further. Most recently some more advancements occurred in Agrobacterium -mediated transformations [ 26 , 42 , 43 ], in order to overcome these limitations, such as transgene-free editing and improvement in the delivery systems in plants [ 35 , 43 , 44 ].…”
Section: Ways To Deliver Crispr/cas Reagents To Plantsmentioning
confidence: 99%
“…The V. cholerae CAST, dubbed INTEGRATE, was later shown to enable site-specific transposon integration in Klebsiella oxytoca, Pseudomonas putida, and Agrobacterium fabrum. , For both CASTs, no host-specific genes are needed, potentially allowing for broader deployment than is typical for other CRISPR-enabled tools used in genome editing. In addition to the high efficiency of insertion, the ease with which the guide-RNA target can be changed to new targets makes this system extremely portable, enabling fast and efficient mutagenesis.…”
Section: Introductionmentioning
confidence: 99%