CRISPR/Cas9‐mediated knockout of a male accessory glands‐specific gene takeout1 decreases the fecundity of Zeugodacus cucurbitae female
Yun Wang,
Hui‐Qian Xu,
Hong‐Liang Han
et al.
Abstract:BACKGROUNDThe melon fly, Zeugodacus cucurbitae (Coquillett), is an invasive Tephritidae pest with robust fertility. The male accessory glands (MAGs) form a vital organ that ensures insect reproductive efficiency. Most of the secreted proteins by MAGs exhibit a male bias expression. Takeout, one of these proteins, is abundantly presented in the MAGs of many insects.RESULTSIn this study, we identified 32 takeout genes in Z. cucurbitae. The phylogenetic analysis and multiple sequence alignment results showed that… Show more
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