Cross‐polarization optical coherence tomographic assessment of in situ simulated erosive tooth wear

Abstract

This clinical study tested crosspolarization optical coherence tomography (CP-OCT) monitoring of erosive tooth wear (ETW). Twenty participants completed a 14-day/arm, 3-arm crossover study simulating different ETW severities. Participants received two enamel specimens (per arm) and were randomized to: severe (s-ETW, lemon juice/pH:2.5/4.25% wt/vol citric acid), moderate (m-ETW, grapefruit juice/pH:3.5/1.03%wt/vol citric acid), and non-ETW (water).Enamel thickness was measured with CP-OCT (day[D] 0, 7, 14) and microcomputed tomography (μ-CT; D14). Enamel surface loss was determined with CP-OCT and optical profilometry (OP; D7, D14). CP-OCT showed higher enamel surface loss for D14 than D7 for m-ETW (P = .009) and s-ETW

Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro

“…However, even if the currently used conditions mimicked that of the in situ study, 11 the magnitude of enamel surface loss (measured by optical profilometry) observed in vitro was higher than in situ (in vitro 15 the saliva buffering and clearing capacities as well as the absence of oral mucosal surfaces for greater fluoride substantivity were not present and is thus a limitation of this in vitro study.…”

“…This study tested a novel model for the in vitro simulation of dental erosion-abrasion, adopted from our in situ ETW model. 10,11 Moreover, it tested the performance of CP-OCT in detecting and monitoring the progression of the simulated in vitro lesions longitudinally. Two objective outcomes were studied: enamel thickness and enamel surface loss, calculated as the change in enamel thickness over time.…”

“…Previous studies have already reported that CP-OCT was useful in assessing these parameters both in vitro 6,8 and in situ. 11 Nevertheless, earlier in vitro studies have only used short-term protocols. 6,8 The current in vitro model simulated ETW lesions similar to those in the more clinically relevant in situ model.…”

“…More recently, it was confirmed that cross-polarization optical coherence tomography (CP-OCT) was suitable for monitoring ETW lesions of different severities formed using the said in situ model. 11 Nevertheless, while in situ studies are undoubtedly more clinically relevant than in vitro experiments, not all research laboratories are equipped to conduct such types of studies. Moreover, when evaluating experimental or novel anti-ETW agents, in vitro models would be more suitable in certain cases.…”

“…In this study, we tested the performance of CP-OCT in assessing ETW lesions of different severities simulated using a novel in vitro dental erosionabrasion (EA) model that mimicked the conditions of our previous in situ ETW model. 11 Objective outcome measures used were enamel thickness and enamel surface loss validated by micro-computed tomography (micro-CT) and optical profilometry, respectively.…”