DNAbarcoding of deep‐water notacanthiform fishes (Teleostei, Elopomorpha)

Abstract

Notacanthiformes Goodrich, 1909, is an order of deep‐sea teleost fishes with a leptocephalus larval stage whose biology and systematics are not widely known. The aim of this work was to apply the DNA barcode standard, based on COI sequence variation, to the delimitation of the species of this order, which is composed by the families Halosauridae (halosaurus) and Notacanthidae (spiny eels). The sequence data used for the analyses were obtained from two sources: 71 samples collected during oceanographic surveys in the North Atlantic Ocean (including the rare species Lipogenys gillii Goode & Bean, 1895) and 95 sequences collected from the BOLD data set. The neighbor‐joining analysis of the barcodes was successful in identifying 96% of the specimens, representing 9 of 16 and 9 of 10 of the recognised species of halosaurus and spiny eels, respectively, including all the current genera. The comparison between the Atlantic and the BOLD data sets also flagged the possibility of occurrences of misidentification and cryptic species. A modern molecular tool like the DNA barcoding supports the previously morphological‐based systematics of the order Notacanthiformes and will provide better access to the taxonomic knowledge of these deep‐water fishes.

Revisión taxonómica de la ictiología marina de Galicia: Clase Actinopteri (Orden Acipenseriformes al Orden Scorpaeniformes)

“…Orden NOTACANTHIFORMES Berg, 1947Familia HALOSAURIDAE Günther, 1868 Halosaurus ovenii Johnson, 1864Halosaurus ovenii Johnson, 1864: Piñeiro et al (1996; BAñón et al (1997); BAñón et al (2010), BAñón et al (2016a,b), BAñón & serrAno (2018) Halosauropsis macrochir (Günther, 1878) Aldrovandia macrochir (Günther, 1878): De Buen (1935a) Halosauropsis macrochir (Günther, 1878): Solórzano et al (1988); roDríguez VillanueVa & Vázquez (1995); Bañón et al (2010), Bañón et al (2016a,b); BarroS garcía et al (2016a); Bañón & Serrano (2018) Aldrovandia phalacra (Vaillant, 1888) Halosaurus phalacrus Vaillant, 1888: De Buen (1935a Aldrovandia phalacra (Vaillant, 1888): Solórzano et al (1988); roDríguez VillanueVa & Vázquez (1995); Bañón et al (2010), Bañón et al (2016a,b); BarroS garcía et al (2016); Bañón & Serrano (2018) Aldrovandia affinis (Günther, 1877) Aldrovandia affinis (Günther, 1877): Bañón et al (2016a,b); BarroS garcía et al (2016a); Bañón & Serrano (2018) Aldrovandia oleosa Sulak, 1977Aldrovandia oleosa Sulak, 1977; BarroS garcía et al (2016a); Bañón & Serrano (2018) Familia NOTACANTHIDAE Rafinesque, 1810 Notacanthus bonaparte Risso, 1840 Notacanthus bonapartei Risso, 1840: Solórzano et al (1983,1988); Piñeiro et al (1996); roDríguez VillanueVa & Vázquez (1995) Notacanthus bonaparte Risso, 1840: Bañón…”

DNA barcoding of Ghanaian fish species: Status and prospects

“…DNA barcoding is a taxonomic method that uses a short genetic marker from a standard region of the DNA sequence of an organism's genome to identify it as belonging to a particular individual or species (Hebert et al, 2003). This short fragment of mitochondrial (650 base pairs) cytochrome c oxidase subunit I (COI) is used to identify the teleost that has been previously described morphologically (Barros-Garcia et al, 2016). The short fragment (DNA sequence) is made of four nucleotide bases A (Adenine), T (Thymine), C (Cytosine) and G (Guanine) (Kaur, 2015).…”

Unveiling taxonomic diversity in the deep‐sea fish genus Notacanthus (Notacanthiformes: Notacanthidae) with description of Notacanthus arrontei n. sp.

“…McCusker et al (2013) found that N. chemnitzii appears to form two distinct genetic clusters in the Pacific and Atlantic oceans. In a first approach to the genus, Barros-García et al (2016) identified four of the six recognized species using DNA barcoding. Subsequently, Poulsen et al (2018) revealed the existence of five MOTUs within N. cf.…”