DNA Nanoengineering and DNA ‐Driven Nanoparticle Assembly

Abstract

Table des matières 1 Introduction ____________________________________________________________ 1 2 From the DNA molecule to nanotechnologies _________________________________ 4 3 DNA nanostructures: from Holliday junctions to 3D origami _____________________ 5 4 DNA-directed assembly of particles: from concepts to the realization of ordered assemblies _________________________________________________________________ 8 4.1 DNA/nanoparticle assembly: primary functionalization strategies ______________ 9 4.2 Towards high-order crystalline structures ________________________________ 11 4.3 Crystallization of heterogeneous systems _________________________________ 13 4.4 DNA/nanoparticle assembly: applications ________________________________ 15 5 Nano-engineering of DNA self-assembled Al/CuO nanothermite _________________ 16 5.1 Fundaments and characterization of DNA/surface chemistry and grafting strategies 17 5.1.1 DNA/alumina interaction evaluation through infrared spectroscopy and first principles calculations ___________________________________________________ 18 5.1.2 Functionalization protocol and colloidal characterization _________________ 20 5.1.3 Quantification of streptavidin and DNA surface densities __________________ 21 5.2 Kinetics of DNA-directed assembly of Al and CuO nanoparticles ______________ 23 5.2.1 Design and impact of the DNA coding sequence _________________________ 24 5.3 Structural and energetic properties of the Al/CuO bionanocomposite __________ 26 6 Conclusion ____________________________________________________________ 29

Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In Vitro

“…This correlates with their lower zeta potentials. It has been proposed that DNA strand bending can occur during such binding events with AuNPs [44]. High degrees of DNA compaction can be unfavorable for transfection.…”