<scp>F</scp>cγ<scp>RI</scp> (<scp>CD</scp>64): An identity card for intestinal macrophages

Calisto, Jaime De; Villablanca, Eduardo J.; Mora, J. Rodrigo

doi:10.1002/eji.201243061

Cited by 25 publications

(17 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, in line with the proposed cascade fashion development, it could be speculated that the presence of the different membrane markers found in the current study, suggests that there is a difference between patients with IBS and the controls in the monocyte/macrophage maturation process. In this sense, the CD11c, 17 CD206, 29 and CX3CR1 17 markers are not expressed in peripheral monocytes, but rather in a state of residence in the bowel, 17 which in our study is very likely associated with the cell-culture conditions. In the bowel, the macrophages express the CD11c 17 marker, although its presence is associated with differentiated macrophages.…”

Section: Discussionmentioning

confidence: 64%

“…Therefore, the general observation of the reduced expression of CD11c in the cells cultured with LPS in our study suggests a maturation process in the presence of a bacterial stimulus, as is to be expected in the cascade proposal of monocyte development. 17 Likewise, the significantly higher MFI of CD11c in M1 control cells cultured without LPS stimulation suggests that, under basal conditions, the cells of the controls are less developed than those from patients with IBS.…”

Section: Discussionmentioning

confidence: 96%

“…CD206 and CX3CR1 are slowly expressed as the cells continue to develop (CD11c -CD206 + , conventionally known as M2), which under the proposal of cascade maturation corresponds to a more advanced state, the same as CX3CR1, whose expression is related to mature phenotypes, mainly immune regulators. 17,26 The proposed maturation cascade is depicted in Figure 1.…”

Section: Flow Cytometrymentioning

confidence: 99%

“…15 This monocyte recruitment provides a constant intestinal replenishment of macrophage populations that gradually mature in a cascade-like manner until they reach the level of resident macrophages. 16,17 Our hypothesis was that patients with IBS may show an alteration in the maturation cascade of monocytes into macrophages in response to bacterial components in charge of triggering this disorder. Therefore our aim was to analyze the maturation phenotype of monocytes/macrophages (CD14+) from the peripheral blood of patients with IBS and controls, by in vitro exposing CD14+ cells to Escherichia coli-derived lipopolysaccharides (LPS).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Maturation Phenotype of Peripheral Blood Monocyte/Macrophage After Stimulation with Lipopolysaccharides in Irritable Bowel Syndrome

Rodríguez-Fandiño

Hernández-Ruiz

López-Vidal

et al. 2017

J Neurogastroenterol Motil

View full text Add to dashboard Cite

Background/AimsAbnormal immune regulation and increased intestinal permeability augmenting the passage of bacterial molecules that can activate immune cells, such as monocytes/macrophages, have been reported in irritable bowel syndrome (IBS). The aim was to compare the maturation phenotype of monocytes/macrophages (CD14+) from IBS patients and controls in the presence or absence of Escherichia coli lipopolysaccharides (LPS), in vitro. MethodsMononuclear cells were isolated from peripheral blood of 20 Rome II-IBS patients and 19 controls and cultured with or without LPS for 72 hours. The maturation phenotype was examined by flow cytometry as follows: M1-Early (CD11c + CD206 -), M2-Advanced (CD11c -CD206 + CX3CR1 + ); expression of membrane markers was reported as mean fluorescence intensity (MFI). The Mann-Whitney test was used and significance was set at P < 0.05. ResultsIn CD14+ cells, CD11c expression decreased with vs without LPS both in IBS (MFI: 8766.0 ± 730.2 vs 12 920.0 ± 949.2, P < 0.001) and controls (8233.0 ± 613.9 vs 13 750.0 ± 743.3, P < 0.001). M1-Early cells without LPS, showed lower CD11c expression in IBS than controls (MFI: 11 540.0 ± 537.5 vs 13 860.0 ± 893.7, P = 0.040), while both groups showed less CD11c in response to LPS (P < 0.01). Furthermore, the percentage of "Intermediate" (CD11c + CD206 + CX3CR1 + ) cells without LPS, was higher in IBS than controls (IBS = 9.5 ± 1.5% vs C = 4.9 ± 1.4%, P < 0.001). Finally, fractalkine receptor (CX3CR1) expression on M2-Advanced cells was increased when treated with LPS in controls but not in IBS (P < 0.001). ConclusionsThe initial phase of monocyte/macrophage maturation appears to be more advanced in IBS compared to controls. However, the decreased CX3CR1 in patients with IBS, compared to controls, when stimulated with LPS suggests a state of immune activation in IBS.

show abstract

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 96%

Section: Flow Cytometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Maturation Phenotype of Peripheral Blood Monocyte/Macrophage After Stimulation with Lipopolysaccharides in Irritable Bowel Syndrome

Rodríguez-Fandiño

Hernández-Ruiz

López-Vidal

et al. 2017

J Neurogastroenterol Motil

View full text Add to dashboard Cite

show abstract

“…The authors showed that macrophages and DCs could clearly be discriminated by CD64 expression, even when the macrophages express hi . More importantly, it has been demonstrated that CD64 can be used as a reliable marker of macrophages in both the small and large intestine under steady-state conditions and inflammatory responses [85] .…”

Section: Cx3cr1mentioning

confidence: 99%

Intestinal barrier: A gentlemen’s agreement between microbiota and immunity

Caricilli

Castoldi

Câmara

2014

WJGP

114

View full text Add to dashboard Cite

Our body is colonized by more than a hundred trillion commensals, represented by viruses, bacteria and fungi. This complex interaction has shown that the microbiome system contributes to the host's adaptation to its environment, providing genes and functionality that give flexibility of diet and modulate the immune system in order not to reject these symbionts. In the intestine, specifically, the microbiota helps developing organ structures, participates of the metabolism of nutrients and induces immunity. Certain components of the microbiota have been shown to trigger inflammatory responses, whereas others, anti-inflammatory responses. The diversity and the composition of the microbiota, thus, play a key role in the maintenance of intestinal homeostasis and explain partially the link between intestinal microbiota changes and gut-related disorders in humans. Tight junction proteins are key molecules for determination of the paracellular permeability. In the context of intestinal inflammatory diseases, the intestinal barrier is compromised, and decreased expression and differential distribution of tight junction proteins is observed. It is still unclear what is the nature of the luminal or mucosal factors that affect the tight junction proteins function, but the modulation of the immune cells found in the intestinal lamina propria is hypothesized as having a role in this modulation. In this review, we provide an overview of the current understanding of the interaction of the gut microbiota with the immune system in the development and maintenance of the intestinal barrier.© 2014 Baishideng Publishing Group Co., Limited. All rights reserved.Key words: Microbiota; Immune system; Lamina propria; Intestinal barrier Core tip: Each of our bodies is colonized by more than a hundred trillion commensals, which include viruses, bacteria and fungi. The association between microbiota and their hosts is complex and has important repercussions for both. The diversity and the composition of the microbiota thus play a key role in the maintenance of intestinal homeostasis and the induction of immunity. These features partially explain the link between alterations in intestinal microbiota and gut-related disorders in humans. In this review, we provide an overview of the current understanding of the interaction between gut microbiota and the immune system in the development and maintenance of the intestinal barrier.Caricilli AM, Castoldi A, Câmara NOS. Intestinal barrier: A gentlemen's agreement between microbiota and immunity.

show abstract

Intestinal Macrophages: Specialized Antigen-Presenting Cells at the Front Line

Denning

Pulendran

2014

Macrophages: Biology and Role in the Pathology of Diseases

View full text Add to dashboard Cite

FcγRI (CD64): An identity card for intestinal macrophages

Cited by 25 publications

References 33 publications

Maturation Phenotype of Peripheral Blood Monocyte/Macrophage After Stimulation with Lipopolysaccharides in Irritable Bowel Syndrome

Maturation Phenotype of Peripheral Blood Monocyte/Macrophage After Stimulation with Lipopolysaccharides in Irritable Bowel Syndrome

Intestinal barrier: A gentlemen’s agreement between microbiota and immunity

Intestinal Macrophages: Specialized Antigen-Presenting Cells at the Front Line

Contact Info

Product

Resources

About