<scp>FCS</scp>‐like zinc finger 6 and 10 repress Sn<scp>RK</scp>1 signalling in Arabidopsis

K, Muhammed Jamsheer; Sharma, Manvi; Singh, Dhriti; Mannully, Chanchal Thomas; Jindal, Sunita; Shukla, Brihaspati N.; Laxmi, Ashverya

doi:10.1111/tpj.13854

Cited by 60 publications

(62 citation statements)

References 66 publications

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“…Through repression of SnRK1, FLZ6 promotes target of rapamycin or TOR signalling pathway that induces growth in favourable conditions. FLZ6 mutants show inhibition and reduced seedling growth under favourable growth conditions due to enhanced SnRK1 activity that confirms role of FLZ6 in plant growth by regulating SnRK1 signalling (Jamsheer et al , 2018). FLZ6 was found to be up-regulated under SHADE at latitude 67°2’N as compared to latitude 56°2’N, which may indicate that the trees in the northern latitudes are better adapted to shade conditions by withstanding the shade stress and continue to grow better compared to the southern populations.…”

Section: Discussionsupporting

confidence: 55%

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

2020

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Study of natural variation is an efficient method to elucidate how plants adapt to local climatic conditions, a key process for the evolution of a species. However, it is challenging to determine the genetic basis of adaptive variation especially in forest trees which have large and complex genomes. Norway spruce is a shade tolerant conifer in which the requirement of far-red light for growth increases latitudinally northwards. In the current work, hypocotyl-length followed a latitudinal cline in response to SHADE (low red:far-red ratio). RNA-sequencing revealed differential gene expression in response to SHADE, between a southern and a northern natural population in Sweden. Exome capture included analysis of uniquely large data set (1654 trees) that revealed missense variations in coding regions of nine differentially expressed candidate genes, which followed a latitudinal cline in allele and genotype frequencies. These genes included five transcription factors involved in vital processes like bud-set/bud-flush, lignin pathway and cold acclimation, and other genes that take part in cell-wall remodeling, secondary cell-wall thickening, response to starvation and immunity. Findings from this work primarily suggests that the northern populations of Norway spruce are better adapted towards disease resistance under shade by up-regulation of lignin pathway that is linked to immunity and it forms concrete basis for local adaptation to light quality in Norway spruce, one of the most economically important conifer tree species in Sweden.

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Section: Discussionsupporting

confidence: 55%

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

2020

Preprint

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“…The in planta analysis identified that FLZ proteins interact with SnRK1 subunits in the common cytoplasmic foci indicating the formation of a complex. The colocalization analysis with a marker protein and stain in this study and a previous study identified that SnRK1-FLZ interaction colocalizes with ER, which provides clues about the biological significance of this complex (14). In most cases, these interactions were found to be located in the proximity of the nucleus suggesting that it might be the regions in ER where ribosomes are studded and mRNA translation is undergoing.…”

Section: Flz Proteins Form Snrk1 Scaffold In Land Plantssupporting

confidence: 63%

“…Functional analysis of FLZ genes identified their roles in the regulation of many SnRK1-regulated processes (11)(12)(13). Consistent with these studies, we recently identified that two members of Arabidopsis FLZ gene family work as negative regulators of SnRK1 signaling (14). Interestingly, many FLZ proteins also showed interaction with RAPTOR, which suggests that they may work as scaffolds by bringing both SnRK1 and TOR complexes in close proximity, which is required for the energy-dependent antagonistic interaction (8,10).…”

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confidence: 60%

“…The expression of FLZ genes is highly regulated by cellular energy levels (76). Indeed, functional analysis with individual FLZ genes identified that they not only work as inert scaffolding proteins but also regulate the level of SnRK1␣1 and hence SnRK1 and TOR activity (14). Energy status and SnRK1 signaling regulate the transcription of FLZ genes (76).…”

Section: Flz Proteins Form Snrk1 Scaffold In Land Plantsmentioning

confidence: 99%

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The FCS-like zinc finger scaffold of the kinase SnRK1 is formed by the coordinated actions of the FLZ domain and intrinsically disordered regions

Shukla

Jindal

et al. 2018

Journal of Biological Chemistry

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The SNF1-related protein kinase 1 (SnRK1) is a heterotrimeric eukaryotic kinase that interacts with diverse proteins and regulates their activity in response to starvation and stress signals. Recently, the FCS-like zinc finger (FLZ) proteins were identified as a potential scaffold for SnRK1 in plants. However, the evolutionary and mechanistic aspect of this complex formation is currently unknown. Here, analyses predicted that FLZ proteins possess conserved intrinsically disordered regions (IDRs) with a propensity for protein binding in the N and C termini across the plant lineage. We observed that the FLZ proteins promiscuously interact with SnRK1 subunits, which formed different isoenzyme complexes. The FLZ domain was essential for mediating the interaction with SnRK1α subunits, whereas the IDRs in the N termini facilitated interactions with the β and βγ subunits of SnRK1. Furthermore, the IDRs in the N termini were important for mediating dimerization of different FLZ proteins. Of note, the interaction of FLZ with SnRK1 was confined to cytoplasmic foci, which colocalized with the endoplasmic reticulum. An evolutionary analysis revealed that in general, the IDR-rich regions are under more relaxed selection than the FLZ domain. In summary, the findings in our study reveal the structural details, origin, and evolution of a land plant-specific scaffold of SnRK1 formed by the coordinated actions of IDRs and structured regions in the FLZ proteins. We propose that the FLZ protein complex might be involved in providing flexibility, thus enhancing the binding repertoire of the SnRK1 hub in land plants.

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“…7 genes were found to be induced in all three data sets. Interestingly, one gene of this category named FLZ8 was found to be a member of the land plant-specific FLZ family implicated in nutrient signaling 26 . FLZ8 physically associates with SnRK1α and β subunits and RAPTOR1B subunit of TOR 27,28 .…”

Section: Data-driven Screening Identifies Potential Negative Feedbackmentioning

confidence: 99%

A negative feedback loop of the TOR signaling moderates growth and enables rapid sensing of stress signals in plants

Jindal

Sharma

et al. 2020

Preprint

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TOR kinase is a central coordinator of nutrient-dependent growth in eukaryotes. Maintaining optimal TOR signaling is critical for the normal development of organisms. However, the mechanisms involved in the maintenance of optimal TOR signaling are currently unknown in plants. In this study, we describe a negative feedback loop of TOR signaling helping in the adaptability of plants in changing environmental conditions. Using an interdisciplinary approach, we identified a plant-specific zinc finger protein FLZ8, as a regulator of TOR signaling in Arabidopsis. In sugar sufficiency, FLZ8 is upregulated by TOR-dependent and -independent histone modifications. FLZ8 negatively regulates TOR signaling by promoting antagonistic SnRK1 alpha1 signaling and bridging the interaction of SnRK1 alpha1 with RAPTOR, a crucial accessory protein of TOR. This negative feedback loop moderates the TOR-growth signaling axis in the favorable condition and helps in the rapid activation of stress signaling in unfavorable conditions establishing its importance in the adaptability of plants.

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FCS‐like zinc finger 6 and 10 repress SnRK1 signalling in Arabidopsis

Cited by 60 publications

References 66 publications

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

Molecular signatures of local adaptation to light in Norway Spruce: role of lignin mediated immunity

The FCS-like zinc finger scaffold of the kinase SnRK1 is formed by the coordinated actions of the FLZ domain and intrinsically disordered regions

A negative feedback loop of the TOR signaling moderates growth and enables rapid sensing of stress signals in plants

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