<scp>FGF</scp>‐2 addition during expansion of human bone marrow‐derived stromal cells alters <scp>MSC</scp> surface marker distribution and chondrogenic differentiation potential

Hagmann, Sébastien; Moradi, Babak; Frank, Sebastian; Dreher, Thomas; Kämmerer, Peer W.; Richter, Wiltrud; Gotterbarm, Tobias

doi:10.1111/cpr.12046

Cited by 58 publications

(44 citation statements)

References 59 publications

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“…[ 43,44 ] On the other hand, FGF-2 can be used to maintain hBM-MSCs in PEG gels in a nonosteogenic differentiated state. Moreover, while it has been shown that FGF-2 not only suppresses dedifferentiation of hBM-MSCs but also enhances their chondrogenic potential [45][46][47][48] in Figure 3 c that 14 d after culture in FGF-2 hBM-MSCs can still be adipogenically and osteogenically differentiated indicating the maintenance of their multipotency.…”

Section: D Msc Monocultures Can Be Osteogenically Differentiated In mentioning

confidence: 88%

Dual Role of Mesenchymal Stem Cells Allows for Microvascularized Bone Tissue‐Like Environments in PEG Hydrogels

Blache

Metzger

Vallmajó-Martín

et al. 2015

Adv Healthcare Materials

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In vitro engineered tissues which recapitulate functional and morphological properties of bone marrow and bone tissue will be desirable to study bone regeneration under fully controlled conditions. Among the key players in the initial phase of bone regeneration are mesenchymal stem cells (MSCs) and endothelial cells (ECs) that are in close contact in many tissues. Additionally, the generation of tissue constructs for in vivo transplantations has included the use of ECs since insufficient vascularization is one of the bottlenecks in (bone) tissue engineering. Here, 3D cocultures of human bone marrow derived MSCs (hBM-MSCs) and human umbilical vein endothelial cells (HUVECs) in synthetic biomimetic poly(ethylene glycol) (PEG)-based matrices are directed toward vascularized bone mimicking tissue constructs. In this environment, bone morphogenetic protein-2 (BMP-2) or fibroblast growth factor-2 (FGF-2) promotes the formation of vascular networks. However, while osteogenic differentiation is achieved with BMP-2, the treatment with FGF-2 suppressed osteogenic differentiation. Thus, this study shows that cocultures of hBM-MSCs and HUVECs in biological inert PEG matrices can be directed toward bone and bone marrow-like 3D tissue constructs.

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Section: D Msc Monocultures Can Be Osteogenically Differentiated In mentioning

confidence: 88%

Dual Role of Mesenchymal Stem Cells Allows for Microvascularized Bone Tissue‐Like Environments in PEG Hydrogels

Blache

Metzger

Vallmajó-Martín

et al. 2015

Adv Healthcare Materials

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“…Using different inducers and methods such as dexamethasone, vitamin C, vitamin D, BMPs, TGF-β, FGF, or insulin, can induce MSCs to differentiate into different lineages such as osteoblasts, chondrocytes, fibroblasts, myoblasts and so on (9,(17)(18)(19)(20)(21)(22). The direction has been set but differentiated cells can reverse the differentiation induced under certain conditions.…”

Section: Discussionmentioning

confidence: 99%

BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures

Xu¹,

Zhou

Guo³

et al. 2018

Exp Ther Med

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Abstract. Bone fractures are a worldwide public health concern. Previous studies have demonstrated that bone morphogenetic protein-7 (BMP7) gene transfer or mesenchymal stem cells (MSCs) transplantation may be a promising novel therapeutic approach. Therefore, the aim of the present study was to observe the effect of bone BMP7 transfer to MSCs on fracture healing. Bone marrow-derived MSCs (BMSCs) from New Zealand white rabbits were isolated and identified using flow cytometry. A recombinant BMP7 overexpressing adenovirus vector (Adv) was constructed and transfected into BMSCs. The expression of BMP7 was detected by reverse transcription-polymerase chain reaction, immunofluorescence and western blotting. The present study additionally investigated the effect of BMP7 on the differentiation capacity of BMSCs. Finally, tissue-engineered bone was created with support material to verify the effect of BMP7-BMSCs on fracture healing. The results demonstrated that the expression of BMP7 was increased at the mRNA and protein levels in BMSCs following transfection with BMP7 overexpressing Adv. The results additionally demonstrated that the expression of BMP7 enhanced the differentiation capacity of bone marrow mesenchymal stem cells and had a promotional effect on fracture healing. Overall, these data suggest that Adv-BMP7 is useful for introducing foreign genes into BMSCs and will be a powerful gene therapy tool for bone regeneration and other tissue engineering applications in the future.

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“…ARS, Alizarin Red S. Color images available online at www .liebertpub.com/tea CD90, CD105, and CD146 were significantly decreased in human BMSCs after treatment with FGF-2. 27 One potential explanation is that FGF-2 could decrease the expression of TGF-b, which is a promoter of cell senescence. 28 Sacchetti et al found that FGF-2 pretreatment decreased the expression of CD105 (endoglin), a TGF-b coreceptor.…”

Section: Discussionmentioning

confidence: 99%

Fibroblast Growth Factor Ligand Dependent Proliferation and Chondrogenic Differentiation of Synovium-Derived Stem Cells and Concomitant Adaptation of Wnt/Mitogen-Activated Protein Kinase Signals

Pizzute

Zhang

et al. 2016

Tissue Engineering Part A

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FGF‐2 addition during expansion of human bone marrow‐derived stromal cells alters MSC surface marker distribution and chondrogenic differentiation potential

Abstract: While holding the benefit of improving MSC chondrogenic differentiation potential, FGF-2 pre-selects certain MSC subtypes. Our data clearly show that expansion culture conditions have a significant effect on distribution of a number of MSC surface markers.

Cited by 58 publications

References 59 publications

Dual Role of Mesenchymal Stem Cells Allows for Microvascularized Bone Tissue‐Like Environments in PEG Hydrogels

Dual Role of Mesenchymal Stem Cells Allows for Microvascularized Bone Tissue‐Like Environments in PEG Hydrogels

BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures

Fibroblast Growth Factor Ligand Dependent Proliferation and Chondrogenic Differentiation of Synovium-Derived Stem Cells and Concomitant Adaptation of Wnt/Mitogen-Activated Protein Kinase Signals

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