<scp>FK</scp>506 regulates pigmentation by maturing the melanosome and facilitating their transfer to keratinocytes

Jung, Hyejung; Chung, Heesung; Chang, Sung Eun; Kang, Duk Hee; Oh, Eok Soo

doi:10.1111/pcmr.12443

Cited by 28 publications

(24 citation statements)

References 41 publications

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“…Our observations partially support the clinical improvement obtained by the combination of calcineurin inhibitors and phototherapy in vitiligo lesions , as topical tacrolimus induces melanocyte migration and maturation . Thus, research on mechanisms aimed at inducing melanocyte migration and/or maturation may provide better results for the UVB‐NB therapy.…”

Section: Discussionsupporting

confidence: 78%

Repigmentation patterns induced by NB‐UVB and their relationship with melanocytic migration and proliferation in vitiligo

Castanedo-Cázares

Cortés-García

Fuentes-Ahumada

et al. 2016

Photoderm Photoimm Photomed

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Section: Discussionsupporting

confidence: 78%

Repigmentation patterns induced by NB‐UVB and their relationship with melanocytic migration and proliferation in vitiligo

Castanedo-Cázares

Cortés-García

Fuentes-Ahumada

et al. 2016

Photoderm Photoimm Photomed

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“…Tacrolimus, another CI, has been shown to promote migration and tyrosinase activity in melanocytes while suppressing proliferation. However, in one study, tacrolimus alone had no effect on migration in cultured melanoblasts, while demonstrating synergistic effects with ET-1 and ET-3 on the promotion of cell migration and melanogenesis [2324]. In addition, tacrolimus increases UVB-mediated melanosome secretion and uptake and enhances the transfer of melanosomes from melanocytes to keratinocytes [24].…”

Section: Discussionmentioning

confidence: 99%

Pimecrolimus increases the melanogenesis and migration of melanocytes in vitro

Chen

Tan

et al. 2017

Korean J Physiol Pharmacol

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Vitiligo is an intriguing depigmentary disorder and is notoriously difficult to be treated. The ultimate goal of vitiligo treatment is to replenish the lost melanocytes by immigration from hair follicle and to restore the normal function of melanogenesis by residual melanocytes. There are two types of topical calcineurin inhibitors called tacrolimus and pimecrolimus, and are recommended as the first-line treatments in vitiligo. Although pimecrolimus is efficacious for the repigmentation of vitiligo, its intrinsic mechanisms have never been investigated in vitro. This research aimed to study the ability of pimecrolimus on stimulating melanogenesis, melanocyte migration and MITF (microphthalmia associated transcription factor) protein expression. Results showed that pimecrolimus at the dosages of 1, 10, 102 nM were neither mitogenic nor cytotoxic to melanocytes. The addition of pimecrolimus at 10, 102 and 103 nM significantly increased intracellular tyrosinase activity, which was consistent with the elevated content of melanin content at the same concentrations. The peak effect was seen at 72 h in response to 102 nM pimecrolimus. Results of the wound scratch assay and Transwell assays indicate that pimecrolimus is effective in facilitating melanocyte migration on a collagen IV-coated surface. In addition, MITF protein yield reached the highest by pimecrolimus at 102 nM. In brief, pimecrolimus enhances melanin synthesis as well as promotes migration of melanocytes directly, possibly via their effects on MITF protein expression.

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“…In brief, after MNT-1 cells (5,000 cells/ well) were incubated for 48 h, medium containing 0.5 mg/ml MTT was added to each well, and the cells were incubated for additional 1 h. The medium was then removed and 100 μl of acidic isopropanol (90% isopropanol, 0.5% sodium dodecyl sulfate (SDS) and 25 mM NaCl) was added to each well. The mean concentration of absorbance at 570 nm in each sample set was measured using a 96-well microtiter plate reader (Dynatech, Chantilly, VA, USA) 31 .…”

Section: Quantitative Real-time Pcr Quantitative Real-time Pcr Was Pmentioning

confidence: 99%

D-tyrosine adds an anti-melanogenic effect to cosmetic peptides

Park

Jung²,

Jang

et al. 2020

Sci Rep

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D-tyrosine is known to negatively regulate melanin synthesis by inhibiting tyrosinase activity. Here, we further reveal that peptides containing terminal D-tyrosine can reduce the melanin contents of human melanocytes. the addition of D-tyrosine to the terminus of the commercial anti-wrinkle peptide, pentapeptide-18 endowed the peptide with the ability to reduce the melanin content and tyrosinase activity in human MNT-1 melanoma cells and primary melanocytes. Consistently, terminal D-tyrosine-containing pentapeptide-18 inhibited the melanogenesis induced by α-MSH treatment or UV irradiation of MNT-1 cells and reduced melanin synthesis in the epidermal basal layer of a 3D human skin model. Furthermore, the addition of D-tyrosine to an anti-aging peptide (GEKG) or an antiinflammatory peptide (GHK) endowed these short peptides with anti-melanogenic effects without altering their intrinsic effects. Together, these data suggest that the addition of D-tyrosine at the terminus of a short cosmetic peptide adds an anti-melanogenic effect to its intrinsic cosmetic effect. Our work offers a novel means of generating dual-function cosmetic peptides.Melanin synthesis occurs in melanocytes and is an essential physiological process that determines the color of human skin and protects its DNA from UV damage 1 . It is closely related with the occurrence of pigmentary disorders 2 : the imbalanced regulation of melanin synthesis results in many pigmentary skin diseases that commonly affect men and women of all ethnic groups 3 , including hyperpigmentation disorders, such as melanocytic nevus, seborrheic keratosis, and melanoma, and hypopigmentation disorders, such as piebaldism, pityriasis, and vitiligo.Melanin is synthesized in melanosomes, which are transferred to surrounding keratinocytes where they protect cells against DNA damage 4,5 . Melanogenesis is critically regulated by the expression of various melanogenesis-related enzymes, such as tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2). Tyrosinase, the rate-limiting enzyme for controlling melanin synthesis, is involved primarily in the production of L-dopaquinone 5,6 . TRP-1 and TRP-2 catalyze specific steps in melanogenesis and stabilize tyrosinase activity 7 . As tyrosinase is a key enzyme that catalyzes a rate-limiting step of melanin synthesis, numerous inhibitors that target tyrosinase have been investigated for their ability to inhibit this process. These include well-known tyrosinase inhibitors, such as hydroquinone 8 , arbutin 9 , kojic acid 10 , and salicylic acid 11 . However, due to the side effects of these inhibitors and the increasing demand for safe and effective cosmetics, many continuing efforts are being made to identify or produce new skin-whitening agents. Some researchers have screened natural products and found that chalcones, resveratrol, and coumarins exhibit inhibitory activity against mushroom tyrosinase 12 . Other groups have sought to develop bioactive materials. Particular attention has been paid to vario...

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FK506 regulates pigmentation by maturing the melanosome and facilitating their transfer to keratinocytes

Cited by 28 publications

References 41 publications

Repigmentation patterns induced by NB‐UVB and their relationship with melanocytic migration and proliferation in vitiligo

Repigmentation patterns induced by NB‐UVB and their relationship with melanocytic migration and proliferation in vitiligo

Pimecrolimus increases the melanogenesis and migration of melanocytes in vitro

D-tyrosine adds an anti-melanogenic effect to cosmetic peptides

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