GPX8 promotes migration and invasion by regulating epithelial characteristics in non‐small cell lung cancer

Abstract

Background Non‐small cell lung cancer (NSCLC) is the most common cause of cancer‐related death worldwide. The family of glutathione peroxidase (GPX), an important antioxidant enzyme in human tissues, has been discovered to play a key role in the development of cancers. GPX8 is the most promising molecule of the family in a therapeutic strategy against a variety of cancers. The main purpose of this study was to examine and analyze the function and clinical value of GPX8 in NSCLC. Methods Immunohistochemistry (IHC), western blot analysis and quantitative real‐time polymerase chain reaction (qPCR) were used to assess GPX8 expression and its clinical significance in NSCLC. A series of cell biology experiments and bioinformatic analysis tools were further used to study the function of GPX8. Results GPX8 expression in tumor tissues was much higher than that in normal lung tissues. High expression of GPX8 in NSCLC was correlated with a worse clinical outcome and prognosis. Furthermore, GPX8 could inhibit the apoptosis of tumor cells and promote its migration and invasion. Conclusions Our results conclusively demonstrated that GPX8 could affect the oncogenesis and prognosis of NSCLC via regulating epithelial characteristics. The study also illustrated that GPX8 could serve as a prognostic predictor and potential therapeutic target for NSCLC.

A cancer stemness-related eight-gene signature for predicting prognosis and malignant degree of lung adenocarcinoma

“…PLEK2 is also involved in cancer invasion and metastasis in gallbladder cancer [26], pancreatic cancer [27], non-small cell lung cancer [28]. Consistent with our ndings, GPX8 is also identi ed as a prognostic biomarker of gastric cancer [29], and is essential for tumor aggressiveness in breast cancer [30], non-small cell lung cancer [31], and gastric cancer [32]. FSCN1 was implicated in nasopharyngeal carcinoma [33] and non-small cell lung cancer [34].…”

The Influence of MicroRNA-31 on Oxidative Stress and Radiosensitivity in Pancreatic Ductal Adenocarcinoma

“…GPx8 is a membrane protein located on the endoplasmic reticulum (ER) and is a molecular gatekeeper that plays a vital role in regulating H 2 O 2 , where the knockdown of GPx8 in HEK-293 cells encourages ER stress and decreased cellular viability [ 37 ]. Zhang et al showed that GPx8 promotes migration and invasion, where high expression of GPx8 in lung cancer was correlated with a worse clinical outcome and prognosis [ 38 ]. Additionally, a recent study demonstrated GPx8 as a critical player in a metabolic-inflammatory pathway that acts as a robust regulator of cancer cell aggressiveness [ 39 ].…”

Structure and Function of Mitochondria‐Associated Endoplasmic Reticulum Membranes (MAMs) and Their Role in Cardiovascular Diseases

“…Glutathione peroxidase 8 (GPX8), another type II transmembrane protein, also localized at MAMs, has a role in the modulation of Ca 2+ dynamics [87]. In particular, GPX8 overexpression causes a decreased ER calcium coupled with a reduction of the ER-mitochondria calcium flux [88]. ER chaperones and folding assistants modulate cell metabolism and survival by controlling ER-mitochondria calcium flux.…”