<scp>HIGLE</scp> is a bifunctional homing endonuclease that directly interacts with <scp>HYL</scp>1 and <scp>SERRATE</scp> in <i>Arabidopsis thaliana</i>

Cho, Seok Keun; Ryu, Moon Young; Poulsen, Carsten Stig; Kim, Jong Hum; Oh, Tae Woo; Choi, Sukwon; Kim, Mi‐Jung; Yang, Jun-Yi; Boo, Kyung Hwan; Geshi, Naomi; Kim, Woo Taek; Yang, Seong Wook

doi:10.1002/1873-3468.12628

Cited by 6 publications

(4 citation statements)

References 46 publications

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“…Consistent with the co-IP assays, COP1-nVenus was associated with both HCS1-cVenus and emitted clear fluorescence in the cytoplasm. We used HIGLE as a HYL1 interacting endonuclease ( 60 ) that showed fluorescence in the nucleus as a positive control ( SI Appendix , Fig. S8 A ).…”

Section: Resultsmentioning

confidence: 99%

HYL1-CLEAVAGE SUBTILASE 1 (HCS1) suppresses miRNA biogenesis in response to light-to-dark transition

Jung

Choi

Boo

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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The core plant microprocessor consists of DICER-LIKE 1 (DCL1), SERRATE (SE), and HYPONASTIC LEAVES 1 (HYL1) and plays a pivotal role in microRNA (miRNA) biogenesis. However, the proteolytic regulation of each component remains elusive. Here, we show that HYL1-CLEAVAGE SUBTILASE 1 (HCS1) is a cytoplasmic protease for HYL1-destabilization. HCS1-excessiveness reduces HYL1 that disrupts miRNA biogenesis, while HCS1-deficiency accumulates HYL1. Consistently, we identified the HYL1K154A mutant that is insensitive to the proteolytic activity of HCS1, confirming the importance of HCS1 in HYL1 proteostasis. Moreover, HCS1-activity is regulated by light/dark transition. Under light, cytoplasmic CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) E3 ligase suppresses HCS1-activity. COP1 sterically inhibits HCS1 by obstructing HYL1 access into the catalytic sites of HCS1. In contrast, darkness unshackles HCS1-activity for HYL1-destabilization due to nuclear COP1 relocation. Overall, the COP1-HYL1-HCS1 network may integrate two essential cellular pathways: the miRNA-biogenetic pathway and light signaling pathway.

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Section: Resultsmentioning

confidence: 99%

HYL1-CLEAVAGE SUBTILASE 1 (HCS1) suppresses miRNA biogenesis in response to light-to-dark transition

Jung

Choi

Boo

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…To date, six major families of homing endonucleases are classified according to their conserved amino acid motifs: LAGLIDADG, H-N-H, His-Cys box, PD-(D/E)-xK, Vsr-like, and GIY-YIG (Belfort & Roberts, 1997;Stoddard, 2005). Additionally, as a new type of endonuclease associated with HYL1 and SE, HIGLE is able to cleave the stem-loop structure of miRNA precursors in vitro , and its cleavage activity seems to be suppressed by HYL1 and SE (Cho et al, 2017). However, no significant difference in HYL1 and SE protein abundance was detected between wild type and atmc1 mutant plants, suggesting that AtMC1 affects miRNA accumulation by not directly modulating the expression of these key miRNA processing factors.…”

Section: Discussionmentioning

confidence: 99%

“…As shown in Figure 5b, At2g30350 encodes HYL1-interacting GIY-YIG DNA endonuclease (HIGLE), which directly interacts with HYL1 and SE to modulate miRNA maturation (Cho et al, 2017). The IR of At2g30350 in atmc1 mutant may affect the biosynthesis of miRNAs.…”

Section: Atmc1 Is Required For Mirna Accumulation In Arabidopsismentioning

confidence: 99%

AtMC1 negatively regulates immunity through modulating pre-mRNA splicing and miRNA accumulation, involving LSM4

Wang

Jiang

et al. 2022

Preprint

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Alternative splicing (AS) of pre-mRNAs is an important gene regulatory mechanism shaping the transcriptome. AtMC1 is an Arabidopsis thaliana type I metacaspase that positively regulates hypersensitive response (HR). Here, we found that AtMC1 is a negative regulator of plant immunity to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 and is physically associated with Sm-like4 (LSM4), which is involved in pre-mRNA splicing. AtMC1 and LSM4 protein levels both increased with their co-expression as compared with their separate expression in vivo. Like AtMC1, LSM4 negatively regulates plant immunity to Pst DC3000 infection. By RNA-seq, AtMC1 was shown to modulate the splicing of many pre-mRNAs including 4CL3, which is a negative regulator of plant immunity. Moreover, atmc1 mutant plants accumulated microRNAs (miRNAs) such as miR398b, miR399a, miR165a and miR159a at a low level than wild-type Arabidopsis Col-0 plants. Furthermore, AtMC1 affected pri-miRNA processing. RNA immunoprecipitation revealed that AtMC1 interacted with these pri-miRNAs in vivo, suggesting that AtMC1 might suppress their processing by directly binding to them. In addition, we found that MC1-mediated plant immunity is conserved in tomato. Thus, AtMC1 plays a regulatory role in both miRNA expression and pre-mRNA splicing, which might contribute to AtMC1-mediated plant immunity.

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“…SLX1 uses the spatial organization of these DNA binding patches to bend the DNA substrate and identify the branching point as a flexible discontinuity in branched DNA substrates ( Figure 4D; Gaur et al, 2019). Based on the available sequences, a GIY-YIG containing, SLX1 like protein called HIGLE has been identified in Arabidopsis (Cho et al, 2017). SLX4 has not been identified in plants so far and is an overly exciting avenue for future research.…”

Section: Slx1mentioning

confidence: 99%

Structural Aspects of DNA Repair and Recombination in Crop Improvement

et al. 2020

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The adverse effects of global climate change combined with an exponentially increasing human population have put substantial constraints on agriculture, accelerating efforts towards ensuring food security for a sustainable future. Conventional plant breeding and modern technologies have led to the creation of plants with better traits and higher productivity. Most crop improvement approaches (conventional breeding, genome modification, and gene editing) primarily rely on DNA repair and recombination (DRR). Studying plant DRR can provide insights into designing new strategies or improvising the present techniques for crop improvement. Even though plants have evolved specialized DRR mechanisms compared to other eukaryotes, most of our insights about plant-DRRs remain rooted in studies conducted in animals. DRR mechanisms in plants include direct repair, nucleotide excision repair (NER), base excision repair (BER), mismatch repair (MMR), non-homologous end joining (NHEJ) and homologous recombination (HR). Although each DRR pathway acts on specific DNA damage, there is crosstalk between these. Considering the importance of DRR pathways as a tool in crop improvement, this review focuses on a general description of each DRR pathway, emphasizing on the structural aspects of key DRR proteins. The review highlights the gaps in our understanding and the importance of studying plant DRR in the context of crop improvement.

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HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

Cited by 6 publications

References 46 publications

HYL1-CLEAVAGE SUBTILASE 1 (HCS1) suppresses miRNA biogenesis in response to light-to-dark transition

HYL1-CLEAVAGE SUBTILASE 1 (HCS1) suppresses miRNA biogenesis in response to light-to-dark transition

AtMC1 negatively regulates immunity through modulating pre-mRNA splicing and miRNA accumulation, involving LSM4

Structural Aspects of DNA Repair and Recombination in Crop Improvement

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