Cannabis sativa L. extract and cannabidiol inhibit in vitro mediators of skin inflammation and wound injury

Abstract

Funding informationLinnea SA; Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR), Grant/ Award Number: Progetto Eccellenza-DiSFeB Skin inflammatory diseases result from complex events that include dysregulation and abnormal expression of inflammatory mediators or their receptors in skin cells. The present study investigates the potential effect of a Cannabis sativa L. ethanolic extract standardized in cannabidiol as antiinflammatory agent in the skin, unraveling the molecular mechanisms in human keratinocytes and fibroblasts. The extract inhibited the release of mediators of inflammation involved in wound healing and inflammatory processes occurring in the skin. The mode of action involved the impairment of the nuclear factor-kappa B (NF-κB) pathway since the extract counteracted the tumor necrosis factor-alpha-induced NF-κB-driven transcription in both skin cell lines. Cannabis extract and cannabidiol showed different effects on the release of interleukin-8 and vascular endothelial growth factor, which are both mediators whose genes are dependent on NF-κB. The effect of cannabidiol on the NF-κB pathway and metalloproteinase-9 (MMP-9) release paralleled the effect of the extract thus making cannabidiol the major contributor to the effect observed. Down-regulation of genes involved in wound healing and skin inflammation was at least in part due to the presence of cannabidiol. Our findings provide new insights into the potential effect of Cannabis extracts against inflammation-based skin diseases.

Mosquitocidal and Anti-Inflammatory Properties of The Essential Oils Obtained from Monoecious, Male, and Female Inflorescences of Hemp (Cannabis sativa L.) and Their Encapsulation in Nanoemulsions

“…To our knowledge, information on the anti-inflammatory effect of hemp EOs is very limited. On the other hand, some authors [45] reported that CBD-enriched extracts can reduce IL-8 and Nf-κB pathways. However, in this study, we demonstrated that the CBD-free hemp EOs did not induce an inflammatory condition, but, additionally, were able to revert an inflammatory condition, reducing the release of the cytokine induced by ETO, on skin cell lines, suggesting that they could be safely used by operators.…”

The Differences in the Proteome Profile of Cannabidiol-Treated Skin Fibroblasts following UVA or UVB Irradiation in 2D and 3D Cell Cultures

“…To observe the effect of CBD, following irradiation cells were incubated for 24 h in medium containing 4 µM of CBD. Used concentration of CBD did not alter the morphology or proliferation of fibroblasts [14] and cells viability measured by MTT test (data not shown). During this incubation the medium did not contain FBS to avoid the influence on proteomic results.…”

<p>Therapeutic Potential of Cannabidiol (CBD) for Skin Health and Disorders</p>

“…A study by Sangiovanni et al reported the effects of CBD and Cannabis Sativa Extract (CSE, standardized to 5% CBD) on human keratinocytes (HaCaT cells) and human dermal fibroblast (HDF) cells. 97 In keratinocytes, TNF-α (Tumor Necrosis Factor alpha) treatment resulted in upregulated expression of 26 genes involved in inflammatory pathways and included chemokines like CXCL8 and CXCL10, interleukins like IL−17C and IL−1B, and VEGF-A. Treatment with CSE downregulated all 26 inflammatory related genes, and CBD alone downregulated 15 genes.…”