<scp><i>Chlorella minutissima</i></scp> grown with xylose and arabinose in tubular photobioreactors: Evaluation of kinetics, carbohydrate production, and protein profile

Freitas, Bárbara Catarina Bastos de; Morais, Michele Greque de; Costa, Jorge Alberto Vieira

doi:10.1002/cjce.24116

Cited by 3 publications

(2 citation statements)

References 45 publications

(66 reference statements)

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“…But even the algae in these cultures decreased their amount of chlorophyll and decreased in size after 24 h. The authors suggest that xylose might be a lipid enhancer in relatively small amounts (Leite et al, 2016). In contrast, Chlorella minutissima was able to grow on a mixture of xylose and arabinose and therefore is a good candidate to convert lignocellulosic residues for biofuel production (Freitas et al, 2021).…”

Section: Nordic Microalgae Tolerate the Presence Of Xylose And Show A...mentioning

confidence: 99%

Utilization of Different Carbon Sources by Nordic Microalgae Grown Under Mixotrophic Conditions

et al. 2022

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Microalgae are promising candidates for sustainable wastewater treatment coupled to the production of biofuel, bioplastic and/or bio-fertilizers. In Nordic countries, however, light is a limiting factor for photosynthesis and biomass production during the winter season. Compared to municipal wastewater, industrial wastewater streams from the pulp and paper industry contain lower amounts of nitrogen, but high concentrations of carbon sources, which could be utilized by microalgae to enhance biomass production in limiting light. This study focused on the utilization of methanol, glycerol and xylose by five different Nordic microalgae [Chlorella vulgaris (13–1), Coelastrella sp. (3–4), Desmodesmus sp. (2–6), Chlorococcum sp. (MC1) and Scotiellopsis reticulata (UFA-2)] grown under mixotrophic conditions. Two of these strains, i.e., Chlorococcum sp. (MC1) and Scotiellopsis reticulata (UFA-2) were able to grow in the presence of xylose or methanol at concentrations of 6 g L–1, or 3%, respectively, in a 12/12 h day/night cycle. HPLC analysis confirmed the consumption of those substrates. Glycerol (2.3 g L–1) was tolerated by all strains and increased growth for Chlorella vulgaris (13–1), while higher concentrations (20 g L–1) were only tolerated by Chlorococcum sp. (MC-1). Fourier-transform infrared spectroscopy, performed after growth in presence of the dedicated carbon source, indicated an increase in the fingerprint region of the carbohydrate fraction. This was particularly the case for Chlorococcum sp. (MC1), when grown in presence of glycerol, and Scotiellopsis reticulata (UFA-2), when grown in presence of xylose. Therefore, these strains could be potential candidates for the production of biofuels, e.g., bioethanol or biogas. We could show that Nordic microalgae are able to grow on various carbon sources; the actual uptake rates are low during a 12/12 h day/night cycle requesting additional optimization of the cultivation conditions. Nonetheless, their potential to use pulp and paper waste-streams for cheap and sustainable biomass production is high and will support the development of new technologies, turning waste-streams into resources in a circular economy concept.

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Section: Nordic Microalgae Tolerate the Presence Of Xylose And Show A...mentioning

confidence: 99%

Utilization of Different Carbon Sources by Nordic Microalgae Grown Under Mixotrophic Conditions

et al. 2022

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“…Small amounts of pentoses (xylose or arabinose) were reported to enhance the growth of Dunaliella salina [33] and Mychonastes homosphaera [34]. However, an inhibitory effect of these sugars on the growth of Coelastrella sp.…”

Section: Comparative Photoautotrophic and Mixotrophic Growth Of Coela...mentioning

confidence: 99%

Microalga Coelastrella sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation

2023

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Improving biomass production with the utilization of low-cost substrate is a crucial approach to overcome the hindrance of high cost in developing large-scale microalgae production. The microalga Coelastrella sp. KKU-P1 was mixotrophically cultivated using unhydrolyzed molasses as a carbon source, with the key environmental conditions being varied in order to maximize biomass production. The batch cultivation in flasks achieved the highest biomass production of 3.81 g/L, under an initial pH 5.0, a substrate to inoculum ratio of 100:3, an initial total sugar concentration of 10 g/L, and a sodium nitrate concentration of 1.5 g/L with continuous light illumination at 23.7 W/m2. The photobioreactor cultivation results indicated that CO2 supplementation did not improve biomass production. An ambient concentration of CO2 was sufficient to promote the mixotrophic growth of the microalga as indicated by the highest biomass production of 4.28 g/L with 33.91% protein, 46.71% carbohydrate, and 15.10% lipid. The results of the biochemical composition analysis suggest that the microalgal biomass obtained is promising as a source of essential amino acids and pigments as well as saturated and monounsaturated fatty acids. This research highlights the potential for bioresource production via microalgal mixotrophic cultivation using untreated molasses as a low-cost raw material.

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Use of Residual Lignocellulosic Biomass and Algal Biomass to Produce Biofuels

Oliveira,

Mescouto,

Paiva

et al. 2024

IJMS

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Efforts are intensifying to identify new biofuel sources in response to the pressing need to mitigate environmental pollutants, such as greenhouse gases, which are key contributors to global warming and various worldwide calamities. Algae and microalgae present themselves as excellent alternatives for solid-gaseous fuel production, given their renewable nature and non-polluting characteristics. However, making biomass production from these organisms economically feasible remains a challenge. This article collates various studies on the use of lignocellulosic waste, transforming it from environmental waste to valuable organic supplements for algae and microalgae cultivation. The focus is on enhancing biomass production and the metabolites derived from these biomasses.

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Chlorella minutissima grown with xylose and arabinose in tubular photobioreactors: Evaluation of kinetics, carbohydrate production, and protein profile

Cited by 3 publications

References 45 publications

Utilization of Different Carbon Sources by Nordic Microalgae Grown Under Mixotrophic Conditions

Utilization of Different Carbon Sources by Nordic Microalgae Grown Under Mixotrophic Conditions

Microalga Coelastrella sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation

Use of Residual Lignocellulosic Biomass and Algal Biomass to Produce Biofuels

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