In vitro Antitumoral Activity of Compounds Isolated from Artemisia gorgonum Webb

Abstract

Artemisia gorgonum (Asteraceae) is an endemic plant to the Cape Verde islands and plays an important role in traditional medicine. The chloroform extract of the plant aerial parts afforded six sesquiterpene lactones, two methoxylated flavonoids, two lignans, and one tetracyclic triterpene, which were isolated by chromatographic methods and their structure established by physical and spectroscopic techniques. The cytotoxic activity of the three major constituents, namely, arborescin, artemetin, and sesamin, was evaluated on neuroblastoma (SH-SY5Y), hepatocarcinoma (HepG2), and nontumoral bone marrow stromal (S17) cell lines. The application of different concentrations of the compounds significantly decreased tumor cells viability at different extents, especially at the highest concentrations tested. Arborescin is the most promising compound as it was able to reduce tumoral cell viability with an IC50 significantly lower (229-233 μM; p < 0.01) than that of S17 cells (445 μM). Arborescin and artemetin were less toxic to nontumoral cells than the antitumoral drug tested, etoposide. Our results indicate that arborescin has a significant cytotoxic activity in vitro, more pronounced on the cancer cell lines, confirming A. gorgonum as a source of potential antitumoral molecules.

Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson

“…Polar solvents are more effective in obtaining phenolic compounds from plants compared to less polar solvents [26]. Less polar solvents such as hexane and CHCl3, carry non-polar and non-phenolic compounds such as terpenoids and methoxylated flavonoids [26,[29][30].For example, in our current study, polar extracts as EtOAc and MeOH extracts had higher total phenolic and flavonoid content than hexane and CHCl3 extracts. The results of present study are similar to those of See et al (2017).…”

Sesquiterpene Lactones fromArtemisiaGenus: Biological Activities and Methods of Analysis

“…Using preparative chromatographic techniques, Martins et al isolated from the chloroform extract of A. gorgonum 11 compounds that included SLs arborescin, arglabin, deacetylglobicin, 2 α -hydroxyarborescin, sanchillin, and hanphyllin. The extract was run over silica gel columns and eluted with ethyl acetate/n-hexane, ethyl acetate/toluene, and dichloromethane/methanol mixtures of increasing polarity [ 119 ].…”

Effects of Artemetin on Nitric Oxide Release and Protection against Peroxidative Injuries in Porcine Coronary Artery Endothelial Cells

“…Regarding this issue it is notable that a wide range of artemetin concentration was used, because no available data are present in the literature about any effect of artemetin in vascular endothelial cells. Anyway, the highest concentrations were similar to those used in cancer cells (Martins et al ., ) and almost corresponding to the doses of artemetin infused in animals (de Souza et al ., ). As performed in all studies by our group regarding NO release in endothelial cells, acetylcholine was used as positive control.…”