Limosilactobacillus fermentum HF06‐derived paraprobiotic and postbiotic alleviate intestinal barrier damage and gut microbiota disruption in mice with ulcerative colitis

Abstract

BACKGROUNDParaprobiotics and postbiotics have shown potential in the treatment of ulcerative colitis (UC). However, their in vivo application is still in its infancy and their mechanisms of action are not well understood.RESULTSHere, we investigated the mitigation effects of the Limosilactobacillus fermentum HF06 derived paraprobiotic (6‐PA) and postbiotic (6‐PS) on dextran sulfate sodium (DSS) induced UC and the potential mechanisms. Results indicated that the administration of 6‐PA and 6‐PS resulted in the inhibition of weight loss and colon shortening in mice with UC. Furthermore, they led to a significant reduction in both fecal moisture content and the levels of pro‐inflammatory cytokines and oxidative stress in the intestine of the mice. 6‐PA and 6‐PS treatment strengthened the intestinal mucosal barrier by dramatically up‐regulating the levels of Zonula occludens‐1 (ZO‐1) and occludin proteins. In addition, 6‐PA and 6‐PS restored intestinal dysbiosis by regulating abundances of some certain bacterias, such as Bifidobacterium, Faecalibaculum, Muribaculaceae, Corynebacterium, Escherichia‐Shigella and Clostridium_ sensu_ stricto_1, and regulated the level of short‐chain fatty acids (SCFAs).CONCLUSIONThese findings illustrated for the first time that L. fermentum HF06 derived paraprobiotic and postbiotic enhanced the intestinal barrier function, and restoring the gut microbiota alterations.This article is protected by copyright. All rights reserved.

Preventive effect of Lacticaseibacillus rhamnosus 2016SWU.05.0601 and its postbiotic elements on dextran sodium sulfate-induced colitis in mice

“…It refers to preparations of inanimate microorganisms and/or their components that are beneficial to the health of the host ( Salminen et al, 2021 ). Recent studies have shown that postbiotics show potential in the treatment of UC, with some studies claiming that probiotics and postbiotics have similar abilities to improve disease phenotypes ( Liu et al, 2023 ). For example, a preclinical study found that Lactobacillus plantarum -derived postbiotics were able to optimize the composition of the intestinal flora and significantly modulate the levels of short-chain fatty acids and neuroactive molecules (5-hydroxytryptamine, γ-aminobutyric acid) in vivo , which in turn ameliorated Salmonella -induced neurological dysfunction ( Wu et al, 2022 ).…”