IbNF‐YA1 is a key factor in the storage root development of sweet potato

Abstract

SUMMARYAs a major worldwide root crop, the mechanism underlying storage root yield formation has always been a hot topic in sweet potato [Ipomoea batatas (L.) Lam.]. Previously, we conducted the transcriptome database of differentially expressed genes between the cultivated sweet potato cultivar “Xushu18,” its diploid wild relative Ipomoea triloba without storage root, and their interspecific somatic hybrid XT1 with medium‐sized storage root. We selected one of these candidate genes, IbNF‐YA1, for subsequent analysis. IbNF‐YA1 encodes a nuclear transcription factor Y subunit alpha (NF‐YA) gene, which is significantly induced by the natural auxin indole‐3‐acetic acid (IAA). The storage root yield of the IbNF‐YA1 overexpression (OE) plant decreased by 29.15–40.22% compared with the wild type, while that of the RNAi plant increased by 10.16–21.58%. Additionally, IAA content increased significantly in OE plants. Conversely, the content of IAA decreased significantly in RNAi plants. Furthermore, real‐time quantitative reverse transcription‐PCR (qRT‐PCR) analysis demonstrated that the expressions of the key genes IbYUCCA2, IbYUCCA4, and IbYUCCA8 in the IAA biosynthetic pathway were significantly changed in transgenic plants. The results indicated that IbNF‐YA1 could directly target IbYUCCA4 and activate IbYUCCA4 transcription. The IAA content of IbYUCCA4 OE plants increased by 71.77–98.31%. Correspondingly, the storage root yield of the IbYUCCA4 OE plant decreased by 77.91–80.52%. These findings indicate that downregulating the IbNF‐YA1 gene could improve the storage root yield in sweet potato.

A Small Auxin-Up RNA Gene, IbSAUR36, Regulates Adventitious Root Development in Transgenic Sweet Potato

“…The GH3 genes are cytosolic, acidic amido synthetases of the firefly luciferase group that conjugate auxins, jasmonates, and benzoate derivatives to a wide group The phytohormone auxin and JA play essential regulatory roles in multiple aspects of plant growth and development and in stress responses [27][28][29]. The YUCCA and TAR members are key enzymes of the IAA-biosynthetic pathway [30,31]. Glycosylation of IAA is carried out by UDP-glycosyltransferase 84B1 (UGT84B1), while UGT74D1 is implicated in the glycosylation of the irreversibly formed IAA catabolite oxIAA in Arabidopsis [32].…”