<scp>III</scp>. Yeast sequencing reports. Sequence of the <i>CDC10</i> region at chromosome III of <i>Saccharomyces cerevisiae</i>

Steensma, H. Yde; Aart, Quirina J. M. Van Der

doi:10.1002/yea.320070412

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…Of the 1,305 bp sequenced, one deviation was found between the wildtype Y574 sequence and that reported by Steensma and van der Aart (1991). We assume that this difference represents a PCR/cioning error in our sequence, because the sequence from the other two cdclO-lO strains matched the published sequence at this position.…”

Section: Sequencing the Cdclo-lo Mutationmentioning

confidence: 69%

Components required for cytokinesis are important for bud site selection in yeast

Flescher

Madden

Snyder

1993

The Journal of Cell Biology

155

150

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Abstract. Polarized cell division is a fundamental process that occurs in a variety of organisms; it is responsible for the proper positioning of daughter cells and the correct segregation of cytoplasmic components. The SPA2 gene of yeast encodes a nonessential protein that localizes to sites of cell growth and to the site of cytokinesis, spa2 mutants exhibit slightly altered budding patterns. In this report, a genetic screen was used to isolate a novel ochre allele of CDCIO, cdclO-lO; strains containing this mutation require the SPA2 gene for growth. CDCIO encodes a conserved potential GTP-binding protein that previously has been shown to localize to the bud neck and to be important for cytokinesis. The genetic interaction of cdclO-lO and spa2 suggests a role for SPA2 in cytokinesis. Most importantly, strains that contain a cdclO-lO mutation and those containing mutations affecting other putative neck filament proteins do not form buds at their norreal proximal location. The finding that a component involved in cytokinesis is also important in bud site selection provides strong evidence for the cytokinesis tag model; i.e., critical components at the site of cytokinesis are involved in determining the next site of polarized growth and division.

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Section: Sequencing the Cdclo-lo Mutationmentioning

confidence: 69%

Components required for cytokinesis are important for bud site selection in yeast

Flescher

Madden

Snyder

1993

The Journal of Cell Biology

155

150

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“…The HmdIII fragments from pVC2a, pVC2b and pVC2c were cloned into pUC19 to facilitate sequencing from universal and reverse primers, and both strands of each clone were sequenced twice. Analysis of these sequences revealed only one point mutation with respect to the published sequence of CDC10 (Steensma & Van der Aart, 1991). Consistent with the mechanism of action of the mutagen used (Cid et al, 1994), there was a G to A change, which implies a substitution of the glycine in position 179 with aspartate in the encoded protein.…”

Section: Resultsmentioning

confidence: 94%

Cell integrity and morphogenesis in a budding yeast septin mutant

et al. 1998

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The non-sporulating diploid strain V327 of Saccharomyces cerevisiae was previously isolated in a search for thermosensitive autolytic mutants. This strain is very efficient a t releasing intracellular proteins into the medium when incubated a t high temperatures. The expression of this lytic phenotype depends on a morphogenetic defect, consisting of the appearance of elongated chains of cells. Transmission electron microscopy revealed a mislocalization of septa at semi-permissive temperatures and a total lack of septation together with abnormal cell wall architecture a t a non-permissive temperature. The septin-encoding CDClO gene was cloned by complementation of the pleiotropic phenotype of the V327 mutant. Rescue and sequencing of CDClO alleles from V327 revealed a point mutation that created a single amino acid change in a region which is well conserved among septins. This new allele was named cdcl0-77. The construction of a cdcl0-ll haploid strain by substituting the CDClO gene with the rescued allele permitted further genetic analyses of the mutation and allowed the construction of new homozygous cdcl0-ll diploid strains that showed a reduced ability to sporulate. Fusing both the wild-type and the cdcl0-ll alleles to green fluorescent protein (GFP) demonstrated that the mutation does not affect the localization of this septin to the bud neck a t the standard growth temperature of 24 OC, although the morphogenetic phenotype a t 37 OC parallels the disappearance of CdclO-GFP a t the ring encircling the septum area.1

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“…Like all of the known septins, Spr3p contains a putative nucleotide-binding domain; however, the function of this domain, and whether it is indeed involved in nucleotide binding, remain to be determined. Like all of the known septins except Cdcl0p (Steensma and van der Aart, 1991;Haarer, B. K., S. R. Ketcham, and J. R. Pringle, unpublished results), C. albicans Cdcl0p (Di-Domenico et al, 1994), and S. pornbe Spn2p (AI-Awar, O., T. Pugh, and J. R. Pringle, unpublished results), Spr3p contains a region near its COOH terminus that is strongly predicted to form a coiled coil. This region in Spr3p is un-usual in consisting of two predicted coiled-coil domains separated by ~50 amino acids, rather than the single, continuous domain found in the other known septins.…”

Section: Discussionmentioning

confidence: 99%

“…In immunofluorescence and immunoelectron microscopy experiments, antibodies specific for the products of these four genes decorate the neck region of wild-type cells in the pattern expected if these proteins are constituents of the neck filaments; moreover, upon shift of any of the four mutants to restrictive temperature, immunofluorescence staining of the necks with each of the four specific antibodies disappears at the same rate as do the filaments as judged by electron microscopy (Haarer and Pringle, 1987;Kim et al, 1991;Ford and Pringle, 1991;Kim, H., B. Haarer, and J. R. Pringle, unpublished results;Mulholland, J., D. Preuss, and D. Botstein, personal communication). Sequencing revealed that Cdc3p, Cdcl0p, Cdcl lp, and Cdcl2p constitute a family of related proteins (25-37% identical in amino acid sequence) (Steensma and van der Aart, 1991;Haarer, B. K., S. H. Lillie, S. K. Ford, S. R. Ketcham, and J. R. Pringle, unpublished results;Longtine et al, 1996). All four proteins contain sequences conserved among nucleotide-binding proteins, a feature shared with the related proteins from other organisms.…”

mentioning

confidence: 99%

Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae.

Fares

Goetsch

Pringle

1996

The Journal of Cell Biology

165

167

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Abstract. The Saccharomyces cerevisiae CDC3, CDCIO, CDCll, and CDC12 genes encode a family of related proteins, the septins, which are involved in cell division and the organization of the cell surface during vegetative growth. A search for additional S. cerevisiae septin genes using the polymerase chain reaction identified SPR3, a gene that had been identified previously on the basis of its sporulation-specific expression. The predicted SPR3 product shows 25-40% identity in amino acid sequence to the previously known septins from S. cerevisiae and other organisms. Immunoblots confirmed the sporulation-specific expression of Spr3p and showed that other septins are also present at substantial levels in sporulating cells. Consistent with the expression data, deletion of SPR3 in either of two genetic backgrounds had no detectable effect on exponentially growing cells. In one genetic background, deletion of SPR3 produced a threefold reduction in sporulation efficiency, although meiosis appeared to be completed normally. In this background, deletion of CDCIO had no detectable effect on sporulation. In the other genetic background tested, the consequences of the two deletions were reversed. Immunofluorescence observations suggest that Spr3p, Cdc3p, and Cdcllp are localized to the leading edges of the membrane sacs that form near the spindle-pole bodies and gradually extend to engulf the nuclear lobes that contain the haploid chromosome sets, thus forming the spores. Deletion of SPR3 does not prevent the localization of Cdc3p and Cdcllp, but these proteins appear to be less well organized, and the intensity of their staining is reduced. Taken together, the results suggest that the septins play important but partially redundant roles during the process of spore formation.

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III. Yeast sequencing reports. Sequence of the CDC10 region at chromosome III of Saccharomyces cerevisiae

Cited by 7 publications

References 27 publications

Components required for cytokinesis are important for bud site selection in yeast

Components required for cytokinesis are important for bud site selection in yeast

Cell integrity and morphogenesis in a budding yeast septin mutant

Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae.

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