<scp>ITS1</scp> and cpb genetic polymorphisms in Algerian and Tunisian <i>Leishmania infantum</i> isolates from humans and dogs

Benikhlef, Razika; Chaouch, Melek; Abid, Meriem Ben; Karim, Abdul; Harrat, Zoubir; Bouratbine, A.; BenAbderrazak, Souha

doi:10.1111/zph.13016

Cited by 2 publications

(2 citation statements)

References 76 publications

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“…MON-80 is less frequent in Spain, and it has been isolated mainly in humans with VL and CL throughout its distribution range (Gállego et al, 2001;Chicharro et al, 2003;Martín-Sánchez et al, 2004;Campino et al, 2006;Pratlong et al, 2013). To the best of our knowledge, only 1 study in North Africa has reported MON-80 in dogs (Benikhlef et al, 2009), and no previous reports describe it in sand flies, although it has been demonstrated experimentally that P. perniciosus could be a potential vector in the outbreak area (Remadi et al, 2018). Therefore, this is the first time that MON-34 and MON-80 strains have been isolated from P. perniciosus and leporids.…”

Section: Discussionmentioning

confidence: 91%

Typing of Leishmania isolates from vectors and leporids of the Madrid (Spain) outbreak

Fernández-Arévalo,

González,

Ballart

et al. 2023

Parasitology

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In 2009, a large outbreak of leishmaniasis, associated with environmental changes, was declared near Madrid (Spain), in which Phlebotomus perniciosus was the vector, whereas the main reservoirs were hares and rabbits. Analysis of isolates from humans, vectors and leporids from the focus identified the Leishmania infantum ITS-Lombardi genotype. However, multilocus enzyme electrophoresis (MLEE), the reference technique for Leishmania typing, and sequencing of the hsp70 gene, a commonly used marker, were not performed. In the present study, 19 isolates from P. perniciosus (n = 11), hares (n = 5) and rabbits (n = 3) from the outbreak area, all characterized as ITS-Lombardi in previous studies, were analysed by MLEE and hsp70 sequencing. The hsp70 results confirmed that all the analysed strains are L. infantum. However, by MLEE, 4 different zymodemes of L. infantum were identified based on variable mobilities of the NP1 enzyme: MON-34 (NP1100, n = 11), MON-80 (NP1130, n = 6), MON-24 (NP1140, n = 1) and MON-331 (NP1150, n = 1). The relative frequency of these zymodemes does not correspond to their usual occurrence in Spain. Moreover, MON-34 and MON-80 were found in P. perniciosus, hares and rabbits for the first time. These findings continue to provide insights into the outbreak and call for further studies with a higher number of strains.

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Section: Discussionmentioning

confidence: 91%

Typing of Leishmania isolates from vectors and leporids of the Madrid (Spain) outbreak

Fernández-Arévalo,

González,

Ballart

et al. 2023

Parasitology

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“…The selection of an appropriate molecular marker was essential to commence the study; there are numerous options for these purposes, such as ITS , CytB , and kDNA ; each has advantages and disadvantages. The genetic marker should be chosen based on the purpose of the study and available samples; even though the first two mentioned markers are more suitable tools for studying genetic diversity [ 22 , 23 ], the significant privilege of kDNA is its high copy number and detectability in samples with low parasite loads [ 24 ], so, kDNA was targeted to improve the detection limit. Leishmania DNA was detected in samples with various titers.…”

Section: Discussionmentioning

confidence: 99%

Detection and phylogenetic analysis of kinetoplast DNA of Leishmania infantum infected humans, domestic dogs and sandflies in Northwest Iran

Behniafar,

Taghipour,

Spotin

et al. 2024

PLoS ONE

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Leishmaniasis refers to a disease with a wide range of manifestations; and there are three main forms of disease, cutaneous, mucocutaneous, and visceral. Leishmaniasis is one of the diseases with a protozoan agent which is vector-borne. Visceral leishmaniasis (VL) is the most severe form that can be fiercely life-threatening if left untreated. VL can be caused by members of Leishmania donovani complex, in Iran, Leishmania infantum is considered the primary causative agent of VL, resulting in a zoonotic form of VL. The two main goals of our work, which followed our prior sero-epidemiological and entomological survey, were to characterize and conduct a phylogenetic analysis of the Leishmania species that infect people, dogs, and sandflies. The samples were collected throughout 2017, from January to December, so blood samples were collected from humans and dogs, while sandfly samples were collected with sticky traps. DNA extracted from all seropositive samples of humans and dogs, 10% of sero-negative human samples, and all collected sandflies were subjected to kDNA-nested-PCR for tracing parasites. A total of 30 samples, including 20 human samples, 8 dog samples, and 2 sandfly samples, were found positive for the kDNA gene of L. infantum. Sequences were evaluated to study the genetic diversity among the six discovered L. infantum. Based on kDNA, the phylogenetic study of L. infantum demonstrated a high level of genetic variety and a relationship between the host, the parasite’s geographic origin, and its genetic diversity.

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ITS1 and cpb genetic polymorphisms in Algerian and Tunisian Leishmania infantum isolates from humans and dogs

Cited by 2 publications

References 76 publications

Typing of Leishmania isolates from vectors and leporids of the Madrid (Spain) outbreak

Typing of Leishmania isolates from vectors and leporids of the Madrid (Spain) outbreak

Detection and phylogenetic analysis of kinetoplast DNA of Leishmania infantum infected humans, domestic dogs and sandflies in Northwest Iran

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