l-Alpha Glycerylphosphorylcholine as a Potential Radioprotective Agent in Zebrafish Embryo Model

Abstract

This work establishes the zebrafish embryo model for ionizing radiation (IR) modifier research and also evaluates the protective effect of L-alpha glycerylphosphorylcholine (GPC). Embryos were exposed to a single-fraction wholebody gamma irradiation (5, 10, 15, and 20 Gy) at different postfertilization time points and were serially assessed for viability and macro-and micromorphologic abnormalities. After toxicity evaluation, 194 lM of GPC was added for certain groups with 3-h incubation before the radiation. Nuclear factor kappa B (NF-jB) and interleukin-1b (IL-1b) expression changes were measured using quantitative real-time polymerase chain reaction. A higher sensitivity could be observed at earlier stages of the embryogenesis. The lethal dose (LD 50 ) for 6 hours postfertilization (hpf) embryos was 15 Gy and for 24 hpf was 20 Gy on day 7, respectively. GPC administration resulted in a significant improvement in both the distortion rate and survival of the 24 hpf embryos. Qualitative evaluation of the histological changes confirmed the protective effect of GPC. IL-1b and NF-jB overexpression due to 10 Gy irradiation was also reduced by GPC. GPC exhibited promising radioprotective effects in our zebrafish embryo model, decreasing the irradiationinduced morphological damage and lethality with significant reduction of IR-caused pro-inflammatory activation.

Efficacy of Long-Term Feeding of α-Glycerophosphocholine for Aging-Related Phenomena in Old Mice

“…com/doi/10.1159/000504962) is a water-soluble choline compound, known to be the product of the deacylation of phosphatidylcholine. GPC is found in some foods, such as milk and soy [1], and its intake has been known to influence brain function and inflammation [2][3][4][5][6][7].…”

New approaches in clinical application of laser-driven ionizing radiation

“…Cells with irregular shapes and a larger hyperchromatic nuclei were observed. These were characterized by pseudomultilayer epithelia and moderate disorganization of the columnar cells, and the cytoplasm was wider in the intestinal lumen [19]. In our present investigations, we started to determine the number of double-strand DNA breaks, using γH2AX immunostaining.…”

“…Apart of the usual in vitro radiation biology endpoints, such as survival evaluation, proteomic and genetic studies, the quantitative assessment of the macro-and micro-morphologic changes, and functional examinations (ECG, EEG, neuro-functional tests, creatinine clearance) could be performed. First, the feasibility of the zebrafish embryo model for investigate the changes in biological effectiveness of chemically modified, or different quality ionizing radiation have been confirmed [19]. The Relative Biological Effectiveness (RBE) of different neutron sources have been defined comparing the radiation effect curves of conventional photon beams to research reactor-based neutron beam, and cyclotron generated neutron source.…”

A novel vertebrate system for the examination and direct comparison of the relative biological effectiveness for different radiation qualities and sources

“…The planned doses were delivered in two portions with half of the dose delivered upwards and the remaining downwards by means of a rotating gantry. The irradiation exposure experimental setup is given in our previous publication (Szab o et al 2016). The embryos were continually assessed after irradiation for survival at 24-hour intervals up to 7 days for the generation of the survival curves and to detect the morphological abnormalities.…”

“…Changes due to total body exposure can also be detected at microenvironmental organ level and the small size of embryos allows for radiobiological experiments at early developmental stage of a new radiation approach with beam size and beam direction restrictions. The zebrafish model has previously been established for radiation biology experiments and radioprotective agents have been tested with this system (Szab o et al 2016). The present study uses the zebrafish embryo system to quantify the low LET and high LET radiationinduced damages at the total organism, at organ and at tissue level in order to establish a novel in vivo model for RBE determination of different high LET sources.…”