<scp>l</scp>-Fucose increases the fucosylation of colorectal cancer cells <i>via</i> promoting the accumulation of serine

Yao, Ye; Qian, Cheng; Chang, Zepeng; Yang, Jiayu; Chen, Yinghan; Wang, Hongchao; Zhu, Jie; Xiao, Yong; Li, Yahui; Zhao, Jianxin; Liu, Wenwei

doi:10.1039/d3fo00820g

Cited by 5 publications

(1 citation statement)

References 45 publications

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“…For the chronic DSS model, 8-week-old mice were administered 2% DSS in autoclaved drinking water for 6 d, followed by regular water for 5 d. This 5-d cycle of 2% DSS was repeated twice, and mice were sacrificed after the last DSS cycle. For the acute DSS model, 8-week-old mice were administered 2.5% DSS in autoclaved drinking water for 6 d. To investigate the effect of l -fucose [ 12 , 44 ], 200 mM l -fucose (Solarbio) dissolved in saline was given to mice during the DSS administration period. To investigate the effect of OXT, mice were intraperitoneally injected with normal saline or OXT (Cayman Chemical Company, 11799; 1 mg/kg per day) during the DSS administration period [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Oxytocin Alleviates Colitis and Colitis-Associated Colorectal Tumorigenesis via Noncanonical Fucosylation

Wang,

Chen,

Guo

et al. 2024

Research

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Colon cancer is increasing worldwide and is commonly regarded as hormone independent, yet recent reports have implicated sex hormones in its development. Nevertheless, the role of hormones from the hypothalamus–hypophysis axis in colitis-associated colorectal cancer (CAC) remains uncertain. In this study, we observed a significant reduction in the expression of the oxytocin receptor (OXTR) in colon samples from both patient with colitis and patient with CAC. To investigate further, we generated mice with an intestinal-epithelium-cell-specific knockout of OXTR. These mice exhibited markedly increased susceptibility to dextran-sulfate-sodium-induced colitis and dextran sulfate sodium/azoxymethane-induced CAC compared to wild-type mice. Our findings indicate that OXTR depletion impaired the inner mucus of the colon epithelium. Mechanistically, oxytocin was found to regulate Mucin 2 maturation through β 1 -3- N -acetylglucosaminyltransferase 7 (B3GNT7)-mediated fucosylation. Interestingly, we observed a positive correlation between B3GNT7 expression and OXTR expression in human colitis and CAC colon samples. Moreover, the simultaneous activations of OXTR and fucosylation by l -fucose significantly alleviated tumor burden. Hence, our study unveils oxytocin’s promising potential as an affordable and effective therapeutic intervention for individuals affected by colitis and CAC.

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Section: Methodsmentioning

confidence: 99%

Oxytocin Alleviates Colitis and Colitis-Associated Colorectal Tumorigenesis via Noncanonical Fucosylation

Wang,

Chen,

Guo

et al. 2024

Research

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D-serine alleviates colitis by regulating intestinal α1,2-fucosylation

Yao,

Pan,

Dai

et al. 2024

Food Bioscience

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Kiwifruit Polysaccharides Alleviate Ulcerative Colitis via Regulating Gut Microbiota-Dependent Tryptophan Metabolism and Promoting Colon Fucosylation

Li,

Han,

Wang

et al. 2024

J. Agric. Food Chem.

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A previous study showed that kiwifruit polysaccharide (KFP) has benefits in relieving intestinal inflammation, while the underlying mechanism remains unresolved. The objective of this study was to investigate the regulatory effect of KFP on the gut microbiota metabolism and intestinal barrier of ulcerative colitis (UC) mice induced by dextran sulfate sodium (DSS). KFP significantly improved the UC symptoms including weight loss, shortened colon length, splenomegaly, diarrhea, hematochezia, and colon inflammation of mice. In addition, KFP could alleviate DSS-caused gut microbiota dysbiosis and increase the levels of shortchain fatty acids in the cecal contents of mice. Furthermore, the results of nontargeted and targeted metabolomics analysis combined with antibiotic treatment revealed that KFP could regulate gut microbiota-dependent tryptophan metabolism, activate the aryl hydrocarbon receptor (AhR) in colon cells, and enhance interleukin-22 production and tight junction proteins' (ZO-1, occludin, and claudin3) expression to repair the intestinal barrier in UC mice. Immunofluorescence results showed that KFP significantly upregulated the conjunction of lectin WGA and UEA1 in the UC mouse colon, implying that KFP promoted fucosylation in the colon. These results suggest that KFP alleviates UC primarily via targeting the gut microbiota involved in the AhR pathway and upregulating colon fucosylation.

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l-Fucose increases the fucosylation of colorectal cancer cells via promoting the accumulation of serine

Abstract: Fucosylation, a kind of posttranslational modification, has been identified as a key regulator of health, with alterations in this process serving as an indicator of diseases such as colorectal cancer....

Cited by 5 publications

References 45 publications

Oxytocin Alleviates Colitis and Colitis-Associated Colorectal Tumorigenesis via Noncanonical Fucosylation

Oxytocin Alleviates Colitis and Colitis-Associated Colorectal Tumorigenesis via Noncanonical Fucosylation

D-serine alleviates colitis by regulating intestinal α1,2-fucosylation

Kiwifruit Polysaccharides Alleviate Ulcerative Colitis via Regulating Gut Microbiota-Dependent Tryptophan Metabolism and Promoting Colon Fucosylation

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