lncRNA TINCR knockdown inhibits colon cancer cells via regulation of autophagy

Abstract

The present study aimed to evaluate the effects of long noncoding (lnc)RNA TINCR ubiquitin domain containing (TINCR) on the development of colon cancer, and the specific underlying mechanisms. The present study used adjacent healthy and cancer tissues obtained from patients with colon cancer and measured lncRNA TINCR expression using reverse transcription‐quantitative (RT‐q) PCR and in situ hybridization assays. Moreover, associations between lncRNA TINCR and clinicopathology and prognosis were also investigated. In addition, the gene and protein expression levels of lncRNA TINCR, mTOR, LC 3B, P62, and Beclin1 were measured using RT‐qPCR and western blotting assays. Cell proliferation, apoptosis, invasion, and migration were measured using MTT, Edu staining, flow cytometry, TUNEL, Transwell, and wound‐healing assays, and cell ultrastructure and LC 3B activation were measured using transmission electron microscopy and cellular immunofluorescence. Results of the present study demonstrated that lncRNA TINCR expression was significantly upregulated in colon cancer tissues, and the overall survival of the low‐expression group was significantly increased, compared with that of the high‐expression groups. In addition, the results of the present study demonstrated that lncRNA TINCR was associated with clinicopathology in patients with colon cancer. Moreover, following lncRNA TINCR knockdown using transfection with small interfering RNA‐TINCR, results of the present study demonstrated that cell proliferation was significantly reduced, while cell apoptosis was significantly increased. In addition, cell invasion and migration were significantly reduced, and autophagy was increased in HT‐29 and SW620 cell lines. However, following treatment with an mTOR agonist (an autophagy inhibitor), biological activities were significantly increased in HT‐29 and SW‐620 cell lines. Collectively, these results demonstrated that lncRNA TINCR may induce colon cancer development through the regulation of autophagy.

Correlation Between Long non-coding RNA SOX2OT rs9839776 C>T Polymorphism and Unexplained Recurrent Miscarriages

“…There are some studies on the number of LncRNA gene polymorphisms associated with various tumors and abnormal expression in many human cancers that have revealed no significant link between their polymorphism and the likelihood of recurrent miscarriage, such as TINCR gene rs2288947 (Y. Liu et al, 2018 (Xu et al, 2023) (Icduygu et al, 2022)(W. , and TOX3 gene that is linked with numerous disorders such as cancer of the pulmonary system, ovarian cancer, breast cancer and polystatic ovarian syndrome (Latif et al, 2010)(W. M. Cao et al, 2016)(X. Zhang et al, 2013)(Gao et al, 2018)(L. Chen et al, 2017 (Bruni et al, 2022).…”

Exploring the prognostic significance of iron death‑related lncRNAs in colorectal cancer: A systematic review and meta‑analysis

“…Colorectal cancer, is a common malignant tumour worldwide, with >1 million new cases each year, accounting for ~10% of new cancer cases, and a mortality rate accounting for 9.4% of all cancer deaths, making it a focus of attention in the field of public health (1,2). Despite considerable progress in multimodal treatment approaches, including precision surgery and tailored chemoradiotherapy, achieving long-term survival and effective disease management poses significant challenges for patients with advanced or highly invasive forms of this cancer (3).…”

lncRNA TINCR promotes the development of cervical cancer via the miRNA‑7/mTOR axis in vitro

“…Long non-coding RNAs (lncRNAs) are non-coding RNAs with a length of >200 nt, which cannot encode proteins but can regulate gene expression through transcriptional, post-transcriptional and epigenetic mechanisms (4). Relevant studies demonstrated that the lncRNA tissue differentiation-inducing non-protein coding RNA (TINCR) participates in tumor progression and is abnormally expressed in various tumor tissues (5)(6)(7)(8). TINCR plays an inhibitory role in prostate cancer (5), whereas it plays a promoting role in other types of tumors including NSCLC, HCC and colon cancer, TINCR is closely correlation with poor prognosis in NSCLC and colon cancer and TINCR overexpression also stimulated cancer cells biological activities including cell proliferation, invasion and migration (6)(7)(8).…”

“…Relevant studies demonstrated that the lncRNA tissue differentiation-inducing non-protein coding RNA (TINCR) participates in tumor progression and is abnormally expressed in various tumor tissues (5)(6)(7)(8). TINCR plays an inhibitory role in prostate cancer (5), whereas it plays a promoting role in other types of tumors including NSCLC, HCC and colon cancer, TINCR is closely correlation with poor prognosis in NSCLC and colon cancer and TINCR overexpression also stimulated cancer cells biological activities including cell proliferation, invasion and migration (6)(7)(8). However, the expression of lncRNA TINCR in cervical cancer and its association with the prognosis of patients, as well as its mechanisms of action in cervical cancer occurrence and development remain unclear.…”