<scp>MALDI</scp>Mass Spectrometry Imaging of Biological Structures

Zaima, Nobuhiro; Goto‐Inoue, Naoko; Hayasaka, Takahiro

doi:10.1002/9780470027318.a9417

Cited by 3 publications

(3 citation statements)

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“…Mass spectrometry imaging (MSI) in contrast is a multiplexed and label-free technique, which can map the distribution and relative abundance of hundreds to thousands of molecules from the same tissue section ( 16 ). Over the last decade, matrix-assisted laser desorption/ionization (MALDI)-MSI has revolutionized spatial biology ( 17 ). The field of neurochemistry has tremendously profited from the ability to detect a variety of molecular species, such as lipids and neurotransmitters, within a single measurement ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

Multiplexed neuropeptide mapping in ant brains integrating microtomography and three-dimensional mass spectrometry imaging

et al. 2023

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Neuropeptides are important regulators of animal physiology and behavior. Hitherto the gold standard for the localization of neuropeptides have been immunohistochemical methods that require the synthesis antibody panels, while another limiting factor has been the brain’s opacity for subsequent in situ light or fluorescence microscopy. To address these limitations, we explored the integration of high-resolution mass spectrometry imaging (MSI) with microtomography for a multiplexed mapping of neuropeptides in two evolutionary distant ant species, Atta sexdens and Lasius niger. For analyzing the spatial distribution of chemically diverse peptide molecules across the brain in each species, the acquisition of serial mass spectrometry images was essential. As a result, we have comparatively mapped the three-dimensional (3D) distributions of eight conserved neuropeptides throughout the brain micro-anatomy. We demonstrate that integrating the 3D MSI data into high-resolution anatomy models can be critical for studying organs with high plasticity such as brains of social insects. Several peptides, like the tachykinin-related peptides TK1 and TK4, were widely distributed in many brain areas of both ant species, whereas others, for instance myosuppressin was restricted to specific regions only. Also, we detected differences at the species level; many peptides were identified in the optic lobe of L. niger, but only one peptide (ITG-like) was found in this region in A. sexdens. Building upon MS-imaging studies on neuropeptides in invertebrate model systems, our approach leverages correlative MSI and computed microtomography for investigating fundamental neurobiological processes by visualizing the unbiased 3D neurochemistry in its complex anatomic environment.

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Section: Introductionmentioning

confidence: 99%

Multiplexed neuropeptide mapping in ant brains integrating microtomography and three-dimensional mass spectrometry imaging

et al. 2023

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“…Mass-spectrometry imaging (MSI) in contrast is a multiplexed and label-free technique, which can map the spatial distribution and relative abundance of hundreds to thousands of molecules from the same tissue section (16). Over the last decade, matrix-assisted laser desorption/ionization (MALDI)-MSI has revolutionized spatial biology (17). The field of neurochemistry has tremendously profited from the ability to detect a variety of molecular species, such as lipids and neurotransmitters, within a single measurement (18).…”

Section: Introductionmentioning

confidence: 99%

Multiplexed neuropeptide mapping in ant brains integrating microtomography and 3D mass spectrometry imaging

Geier

Mansilla

Liutkevičiūtė

et al. 2022

Preprint

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Neuropeptides are important regulators of animal physiology and behavior. Hitherto large-scale localization of neuropeptides mainly relied on immunohistochemical methods requiring the availability of antibody panels, while another limiting factor has been the brain's opacity for subsequent light or fluorescence microscopy. To address these limitations, we integrated high-resolution mass spectrometry imaging (MSI) with microtomography for a multiplexed mapping of neuropeptides in two evolutionary distant ant species, Atta sexdens and Lasius niger. For analyzing the spatial distribution of chemically diverse peptide molecules across the brain in each species, the acquisition of serial mass spectrometry images was essential. As a result, we have comparatively mapped the 3D distributions of eight conserved neuropeptides throughout the brain micro-anatomy. We demonstrate that integrating the 3D MSI data into high-resolution anatomy models can be critical for studying organs with high plasticity such as brains of social insects. Several peptides, like the tachykinin-related peptides TK1 and TK4, were widely distributed in many brain areas of both ant species, whereas others, for instance myosuppressin was restricted to specific regions only. Also, we detected differences at the species level; many peptides were identified in the optic lobe of L. niger, but only one peptide (ITG-like) was found in this region in A. sexdens. Our approach provides the basis for investigating fundamental neurobiological processes by visualizing the unbiased 3D neurochemistry in its complex anatomic environment. Significance statement Until recently, the inability to detect entire molecules such as neuropeptides within their spatial biological context and simultaneously link their occurrence to anatomically and physiologically relevant areas has limited our understanding of complex neurochemical processes. This situation has now changed dramatically with the optimization of a new multiplexed imaging method based on mass spectrometry, which enables us to study previously invisible processes at the microscopic scale. With the marriage of mass spectrometry imaging and microtomography, we show that it has become possible to build high-resolution maps of neuropeptides in complex anatomical structures as small as ant brains. These maps, embedded in the 3D neuroanatomy, expand the understanding of the spatial organization of brain chemistry and provide a baseline for neurobiological and neurochemical studies.

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“…There exist techniques that can be used to deposit highly uniform biopolymers thin films and micro-patterns [59], like laser-induced forward transfer (LIFT) [60] or Matrix-assisted laser desorption/ionization (MALDI) [61]. Besides, sol-gel, layer by layer (LbL), aerosol spraying, dip coating, and spin coating are techniques that entail liquid solutions of the material in a volatile solvent [7,62,63].…”

Section: This Chapter Introduces An Innovative Solution For the Synthmentioning

confidence: 99%

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Axente¹,

Sima²,

Ristoscu³

et al. 2016

Recent Advances in Biopolymers

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This chapter provides an overview of recent advances in the field of laser-based synthesis of biopolymer thin films for biomedical applications. The introduction addresses the importance of biopolymer thin films with respect to several applications like tissue engineering, cell instructive environments, and drug delivery systems. The next section is devoted to applications of the fabrication of organic and hybrid organic-inorganic coatings. Matrix-assisted pulsed laser evaporation (MAPLE) and Combinatorial-MAPLE are introduced and compared with other conventional methods of thin films assembling on solid substrates. Advantages and limitations of the methods are pointed out by focusing on the delicate transfer of bio-macromolecules, preservation of properties and on the prospect of combinatorial libraries' synthesis in a single-step process. The following section provides a brief description of fundamental processes involved in the molecular transfer of delicate materials by MAPLE. Then, the chapter focuses on the laser synthesis of two polysaccharide thin films, namely Dextran doped with iron oxide nanoparticles and Levan, followed by an overview on the MAPLE synthesis of other biopolymers. The chapter ends with summary and perspectives of this fast-expanding research field, and a rich bibliographic database.

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MALDIMass Spectrometry Imaging of Biological Structures

Cited by 3 publications

References 113 publications

Multiplexed neuropeptide mapping in ant brains integrating microtomography and three-dimensional mass spectrometry imaging

Multiplexed neuropeptide mapping in ant brains integrating microtomography and three-dimensional mass spectrometry imaging

Multiplexed neuropeptide mapping in ant brains integrating microtomography and 3D mass spectrometry imaging

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

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