MicroRNA‐195‐5p suppresses osteosarcoma cell proliferation and invasion by suppressing naked cuticle homolog 1

Abstract

MiR-195-5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR-195-5p in osteosarcoma development remains unclear. In this study, real-time PCR was performed to examine the miR-195-5p expression in human osteosarcoma cell lines. CCK-8 assay and Transwell assay were carried out to measure the effect of miR-195-5p on cell proliferation and invasion. Luciferase reporter assay was used to identify the targets of miR-195-5p. The results showed that miR-195-5p was significantly downregulated in osteosarcoma cells. Forced expression of miR-195-5p significantly inhibited cell proliferation, suppressed cell migration and invasion, compared with wild-type and control-transfected osteosarcoma cells. Luciferase reporter assay revealed that miR-195-5p binds to the 3'-untranslated region (UTR) of Naked cuticle homolog 1 (NKD1), indicating that NKD1 was a novel target of miR-195-5p. NKD1 mRNA and protein levels were reduced after overexpression of miR-195-5p. Moreover, silencing of NKD1 significantly inhibited the proliferation and invasion of osteosarcoma cells. Accordingly, our results support a tumor suppressor role of miR-195-5p in osteosarcoma through inhibiting NKD1, and it may be a promising therapeutic target for osteosarcoma.

Retracted: Long non‐coding RNA XIST promotes osteosarcoma progression by targeting YAP via miR‐195‐5p

“…Studies have reported that miR‐195‐5p plays an important role in inhibiting the development of various human cancers, including bladder, cervical, and breast cancers . MiR‐195‐5p can suppress OS cell metastasis by targeting CCND1 and can also suppress OS cell proliferation and invasion by suppressing naked cuticle homolog 1 . However, current studies have been unable to clarify the exact relationship between XIST and miR‐195‐5p.…”

Identification of differentially expressed miRNAs in differentiating benign from malignant pleural effusion

“…Another important miRNA identified in this study was over-expressed miR-34a-5p in MPE group which showed a malignant tendency in lesions. This statement was supported by some previous publications [22][23][24]. In non-small cell lung cancer, high-expression of miR-34a-5p can induce cell apoptosis by targeting MDM4, and increase p53 and p21 protein expressions [16].…”

“…Another target gene of miR-195-5p AGO4, as an microRNA machinery gene, was demonstrated to be upregulated in ovarian carcinogenesis and apoptosis process [15]. In addition, miR-195-5p has been widely identified as tumor suppressor in osteosarcoma, prostate cancer, and bladder cancer [4,7,23].…”

MicroRNA‑376a inhibits cell proliferation and invasion in osteosarcoma via directly targeting SATB1

“…Aberrantly expressed miRs are implicated in the regulation of OS onset and development (23)(24)(25). Therefore, key miRs in OS must be identified to develop promising and effective therapeutic targets for patients with OS.…”