<scp>microRNA</scp>‐binding proteins: specificity and function

Zealy, Richard W.; Wrenn, Samuel P.; Davila, Sylvia; Min, Kyung‐Won; Yoon, Je‐Hyun

doi:10.1002/wrna.1414

Cited by 74 publications

(56 citation statements)

References 63 publications

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“…Furthermore, abnormally expressed miRNAs in tumors are well concerned [14,16]. Functionally, miRNAs target on the corresponding mRNAs, and thus degrade them or inhibit their translation [14,15]. It is estimated that over 30% of human genes and cellular processes are regulated or controlled by miRNAs [9,16].…”

Section: Discussionmentioning

confidence: 99%

“…The cancer transcriptome is characterized by dysregulation of both protein-coding and noncoding transcripts [12,15]. Competitive endogenous RNA (ceRNA) networks have been hypothesized, in which some functional RNAs can modulate each other's transcription using miRNA response elements (MREs) [22].…”

Section: Discussionmentioning

confidence: 99%

“…MiR-NAs are a class of endogenous non-coding RNA (about 19-24 nucleotides in length) that regulate gene expression through complete or incomplete pairing with complementary nucleotide sequences of the target mRNA 3′-terminal non-translation region (3′-UTR) after transcription [13,14]. These miRNA networks regulate the expression of more than 30% of cellular proteins [15]. Previous studies have shown that miRNAs could be involved in a wide range of cell biological processes, including cell proliferation, differentiation, apoptosis, and metastasis and so on [14,16].…”

Section: Introductionmentioning

confidence: 99%

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miR-450b-3p inhibited the proliferation of gastric cancer via regulating KLF7

et al. 2020

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Background: This study aimed to investigate the clinical characteristics of miR-450b-3p in the patients of gastric cancer (GC), and further explore whether miR-450b-3p could inhibit the proliferation of GC cells via regulating KLF7. Methods:Real-time quantitative PCR (qRT-PCR) was performed to detect the expression level of miR-450b-3p in 48 GC patients of tumor tissue and paracancerous tissue specimens collected, and the associations between miR-450b-3p and the clinical characteristics of GC patients were analyzed. Meanwhile, the expression of miR-450b-3p in GC cell lines was verified using qRT-PCR. miR-450b-3p overexpression vectors was constructed in GC cell lines including AGS and BGC-823, and then CCK-8 cell proliferation assay, Plate colony formation assay and EdU assay were applied to analyze the biological function of miR-450b-3p in GC cell lines. Results:The results of qRT-PCR showed that the expression level of miR-450b-3p in GC tissues was lower than that in paracancerous tissues, and the difference was statistically significant. Compared with GC patients with high-miR-450b-3p expression, these GC patients with low-miR-450b-3p expression had a higher pathological stage and tumor size. Subsequently, the proliferation ability of GC cells in miR-450b-3p mimic was significantly decreased when comparing with the NC mimic. In addition, qRT-PCR indicated that the expression level of KLF7 significantly decreased after miR-450b-3p mimic. Therefore, it was demonstrated that miR-450b-3p might inhibit the malignant progression of GC via modulating KLF7. Bioinformatics analysis and dual luciferase reporter suggested miR-450b-3p was bound to KLF7. Finally, the results of the reverse experiment confirmed that overexpression of KLF7 could reverse miR-450b-3p mimic induced-inhibition of GC malignant progression. Conclusions:Generally, miR-450b-3p significantly down-regulated in GC tissues and cell lines, and was associated with the pathological stage and tumor size of GC patients. Meanwhile, miR-450b-3p inhibited cell proliferation in GC via modulating KLF7.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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miR-450b-3p inhibited the proliferation of gastric cancer via regulating KLF7

et al. 2020

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“…Although these RBPs have been known to play important cellular roles by interacting with mRNAs (Gerstberger et al 2014;Dixit and Lukeš 2018;Moore and von Lindern 2018), recent work has demonstrated the importance of RBPs and their interactions with various types of noncoding RNA such as microRNAs, small nucleolar RNAs, long-noncoding RNA, piRNA, and viral RNA (Bagni and Lapeyre 1998;Jiang and Coller 2012;Ciafrè and Galardi 2013;Falaleeva et al 2016;Zealy et al 2017;Grzechnik et al 2018;Lei et al 2018;Tedeschi et al 2018;Baou et al 2011;Zhou et al 2018;Qiao et al 2019;Yang et al 2019). The formation of RNP complexes acts to regulate essential cellular functions from cell division to cell death, transcription to post-translational gene regulation, and downstream functions (Clayton and Shapira 2007;Glisovic et al 2008;Kishore et al 2010;Brinegar and Cooper 2016).…”

Section: Introductionmentioning

confidence: 99%

Current approaches for RNA-labelling to identify RNA-binding proteins

Gemmill

D’souza

Meier-Stephenson

et al. 2020

Biochem. Cell Biol.

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RNA is involved in all domains of life, playing critical roles in a host of gene expression processes, host-defense mechanisms, cell proliferation, and diseases. A critical component in many of these events is the ability for RNA to interact with proteins. Over the past few decades, our understanding of such RNA–protein interactions and their importance has driven the search and development of new techniques for the identification of RNA-binding proteins. In determining which proteins bind to the RNA of interest, it is often useful to use the approach where the RNA molecule is the “bait” and allow it to capture proteins from a lysate or other relevant solution. Here, we review a collection of methods for modifying RNA to capture RNA-binding proteins. These include small-molecule modification, the addition of aptamers, DNA-anchoring, and nucleotide substitution. With each, we provide examples of their application, as well as highlight their advantages and potential challenges.

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“…miRNA are small, non-coding RNA that regulate gene expression post-transcriptionally through complementary binding to sequences in the 3′untranslated region of target mRNA, resulting in translational inhibition [ 10 ]. After bacterial infection, miRNA regulate host responses by modulating the expression of genes involved in a variety of cellular processes such as proliferation, cell growth, cell death, inflammation and development [ 11 , 12 ]. Some in vitro and in vivo studies have reported altered miRNA expression following Salmonella infection.…”

Section: Introductionmentioning

confidence: 99%

Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection

Herrera-Uribe

Zaldívar‐López

Aguilar

et al. 2018

Vet Res

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Salmonellosis is a gastrointestinal disease caused by non-typhoidal Salmonella serovars such as Salmonella Typhimurium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast reservoir for disease. After intestinal colonization, Salmonella Typhimurium reaches mesenteric lymph nodes (MLN), where infection is controlled avoiding systemic spread. Although the molecular basis of this infection has been extensively studied, little is known about how microRNA (miRNA) regulate the expression of proteins involved in the Salmonella-host interaction. Using small RNA-seq, we examined expression profiles of MLN 2 days after infection with Salmonella Typhimurium, and we found 110 dysregulated miRNA. Among them, we found upregulated miR-21, miR-155, miR-150, and miR-221, as well as downregulated miR-143 and miR-125, all of them previously linked to other bacterial infections. Integration with proteomic data revealed 30 miRNA potentially regulating the expression of 15 proteins involved in biological functions such as cell death and survival, inflammatory response and antigenic presentation. The inflammatory response was found increased via upregulation of miRNA such as miR-21 and miR-155. Downregulation of miR-125a/b, miR-148 and miR-1 were identified as potential regulators of MHC-class I components PSMB8, HSP90B1 and PDIA3, respectively. Furthermore, we confirmed that miR-125a is a direct target of immunoproteasome component PSMB8. Since we also found miR-130 downregulation, which is associated with upregulation of HSPA8, we suggest induction of both MHC-I and MHC-II antigen presentation pathways. In conclusion, our study identifies miRNA that could regulate critical networks for antigenic presentation, inflammatory response and cytoskeletal rearrangements.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0506-1) contains supplementary material, which is available to authorized users.

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microRNA‐binding proteins: specificity and function

Cited by 74 publications

References 63 publications

miR-450b-3p inhibited the proliferation of gastric cancer via regulating KLF7

miR-450b-3p inhibited the proliferation of gastric cancer via regulating KLF7

Current approaches for RNA-labelling to identify RNA-binding proteins

Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection

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