<scp>MicroRNAs</scp> balance growth and salt stress responses in sweet sorghum

Zheng, Hou-Feng; Li, Simin; Gao, Yinping; Dang, Yingying; Chen, Zengting; Wu, Fenghui; Wang, Xuemei; Xie, Qi; Sui, Na

doi:10.1111/tpj.16065

Cited by 11 publications

(3 citation statements)

References 104 publications

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“…MiRNA plays a crucial role in responding to different stress conditions by regulating target genes [ 40 ]. miRNA was found to be involved in the response to salt stress in sorghum, sweet potato, maize, and rice [ 41 , 42 , 43 , 44 ]. The involvement of MiRNA has also been reported in the regulation of salt stress in wheat in recent studies.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of miRNA Expression Profiles under Salt Stress in Wheat

Qiao

Jiao

Wang

et al. 2023

Genes

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Salt stress is one of the important environmental factors that inhibit the normal growth and development of plants. Plants have evolved various mechanisms, including signal transduction regulation, physiological regulation, and gene transcription regulation, to adapt to environmental stress. MicroRNAs (miRNAs) play a role in regulating mRNA expression. Nevertheless, miRNAs related to salt stress are rarely reported in bread wheat (Triticum aestivum L.). In this study, using high−throughput sequencing, we analyzed the miRNA expression profile of wheat under salt stress. We identified 360 conserved and 859 novel miRNAs, of which 49 showed considerable changes in transcription levels after salt treatment. Among them, 25 were dramatically upregulated and 24 were downregulated. Using real−time quantitative PCR, we detected significant changes in the relative expression of miRNAs, and the results showed the same trend as the sequencing data. In the salt−treated group, miR109 had a higher expression level, while miR60 and miR202 had lower expression levels. Furthermore, 21 miRNAs with significant changes were selected from the differentially expressed miRNAs, and 1023 candidate target genes were obtained through the prediction of the website psRNATarget. Gene ontology (GO) analysis of the candidate target genes showed that the expressed miRNA may be involved in the response to biological processes, molecular functions, and cellular components. In addition, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis confirmed their important functions in RNA degradation, metabolic pathways, synthesis pathways, peroxisome, environmental adaptation, global and overview maps, and stress adaptation and the MAPK signal pathway. These findings provide a basis for further exploring the function of miRNA in wheat salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of miRNA Expression Profiles under Salt Stress in Wheat

Qiao

Jiao

Wang

et al. 2023

Genes

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“…Increases in soil and water salinity negatively impact growth, development, and yield of different crops around the globe ( El Sabagh et al., 2021 ; Saddiq et al., 2021 [wheat; Triticum aestivum L.]; Ali et al., 2021 ; Khan et al., 2022 [rice; Oryza sativa L.]; Zahra et al., 2020 ; Zakavi et al., 2022 [maize; Zea mays L.]; Ehtaiwesh, 2022 [barley Hordeum vulgare L]; Mulaudzi et al., 2022 ; Sun et al., 2023 [sorghum Sorghum bicolor (L.) Moench]) and is a risk to food security ( Fatima et al., 2020 ; Mukhopadhyay et al., 2021 ). It is projected that over 800 million hectares of land will be affected by salinity in the near future.…”

Section: Introductionmentioning

confidence: 99%

Response of winter wheat genotypes to salinity stress under controlled environments

Ehtaiwesh,

Sunoj,

Djanaguiraman

et al. 2024

Front. Plant Sci.

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This study was conducted in controlled environmental conditions to systematically evaluate multi-traits responses of winter wheat (Triticum aestivum L.) genotypes to different salinity levels. Responses were assessed at the germination to early seedling stage (Experiment 1). Seeds of different genotypes (n=292) were subjected to three salinity levels (0 [control], 60, and 120 mM NaCl). Principal Component Analysis (PCA) revealed that among studied traits seedling vigor index (SVI) contributed more towards the diverse response of genotypes to salinity stress. Based on SVI, eight contrasting genotypes assumed to be tolerant (Gage, Guymon, MTS0531, and Tascosa) and susceptible (CO04W320, Carson, TX04M410211) were selected for further physio-biochemical evaluation at the booting stage (Experiment 2) and to monitor grain yield. Higher level of salinity (120 mM NaCl) exposure at the booting stage increased thylakoid membrane damage, lipid peroxidation, sugars, proline, and protein while decreasing photosynthesis, chlorophyll index, starch, and grain yield. Based on grain yield, the assumed magnitude of the genotypic response shown in Experiment 1 was not analogous in Experiment 2. This indicates the necessity of individual screening of genotypes at different sensitive growth stages for identifying true salinity-tolerant and susceptible genotypes at a particular growth stage. However, based on higher grain yield and its least percentage reduction under higher salinity, Guymon and TX04M410211 were identified as tolerant, and Gage and CO04W320 as susceptible at the booting stage, and their biparental population can be used to identify genomic regions for booting stage-specific salinity response.

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“…Plant microRNAs (miRNAs) are a class of conserved endogenous small non‐coding RNAs, play pivotal roles in various developmental processes, signal transduction, plant hormone responses and stress resistance (Begum, 2022; Liu et al ., 2021; Ma and Hu, 2023; Meyers and Axtell, 2019; Sun et al ., 2023). MiR156, the first miRNA identified in plants (Duan et al ., 2022; Jerome Jeyakumar et al ., 2020; Zheng et al ., 2019), has been found to regulate both grain yield and salt tolerance in rice by targeting the key gene Ideal Plant Structure 1 ( IPA1 ) (Jia et al ., 2022; Jiao et al ., 2010; Wang and Wang, 2017).…”

Section: Introductionmentioning

confidence: 99%

miR396b/GRF6 module contributes to salt tolerance in rice

Yuan,

Cheng,

Wang

et al. 2024

Plant Biotechnology Journal

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SummarySalinity, as one of the most challenging environmental factors restraining crop growth and yield, poses a severe threat to global food security. To address the rising food demand, it is urgent to develop crop varieties with enhanced yield and greater salt tolerance by delving into genes associated with salt tolerance and high‐yield traits. MiR396b/GRF6 module has previously been demonstrated to increase rice yield by shaping the inflorescence architecture. In this study, we revealed that miR396b/GRF6 module can significantly improve salt tolerance of rice. In comparison with the wild type, the survival rate of MIM396 and OE‐GRF6 transgenic lines increased by 48.0% and 74.4%, respectively. Concurrent with the increased salt tolerance, the transgenic plants exhibited reduced H2O2 accumulation and elevated activities of ROS‐scavenging enzymes (CAT, SOD and POD). Furthermore, we identified ZNF9, a negative regulator of rice salt tolerance, as directly binding to the promoter of miR396b to modulate the expression of miR396b/GRF6. Combined transcriptome and ChIP‐seq analysis showed that MYB3R serves as the downstream target of miR396b/GRF6 in response to salt tolerance, and overexpression of MYB3R significantly enhanced salt tolerance. In conclusion, this study elucidated the potential mechanism underlying the response of the miR396b/GRF6 network to salt stress in rice. These findings offer a valuable genetic resource for the molecular breeding of high‐yield rice varieties endowed with stronger salt tolerance.

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MicroRNAs balance growth and salt stress responses in sweet sorghum

Cited by 11 publications

References 104 publications

Comparative Analysis of miRNA Expression Profiles under Salt Stress in Wheat

Comparative Analysis of miRNA Expression Profiles under Salt Stress in Wheat

Response of winter wheat genotypes to salinity stress under controlled environments

miR396b/GRF6 module contributes to salt tolerance in rice

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