miR‐203a‐3p promotes loureirin A‐induced hair follicle stem cells differentiation by targeting Smad1

Abstract

MicroRNAs (miRNAs) participate in the repair of skin trauma. Our previous study indicated that loureirin A promoted hair follicle stem cells (HFSCs) to repair skin epidermis. However, the mechanism of miRNA‐mediated regulation of loureirin A‐induced HFSC differentiation remained to be explored. In the present study, HFSCs from rat vibrissa were identified by immunofluorescence in vitro. Microarray and quantitative real time polymerase chain reaction analyses demonstrated that miR‐203a‐3p was upregulated in differentiated HFSCs induced by loureirin A. The expression of cytoskeletal keratin (CK) 5 and involucrin was promoted by miR‐203a‐3p mimics while repressed by a miR‐203a‐3p inhibitor. Smad1 was identified as a key target of miR‐203a‐3p using target prediction tools. Luciferase reporter gene test confirmed a special target association between miR‐203a‐3p and Smad1. Short interfering Smad1 was transfected into HFSCs, and the expression levels of CK5 and involucrin were upregulated. Thus, it can be inferred that miR‐203a‐3p negatively regulated the expression of Smad1 and promoted the differentiation of loureirin A‐induced HFSCs. Bone morphogenetic protein (BMP) signal inhibition and Wnt activation coregulate skin injury repair. BMP/Smad1 signaling is involved in maintaining the characteristics of HFSCs and inhibiting their differentiation. Our results showed that miR‐203a‐3p reduces Smad1 to release BMP inhibition. Taken together, miR‐203a‐3p/Smad1 is a potential therapeutic molecular target in skin wound healing, and may play an active role in wound repair and regenerative medicine.

Perspectives on miRNAs Targeting DKK1 for Developing Hair Regeneration Therapy

“…Furthermore, Ma et al have revealed that miR-203 may regulate goat HF development [ 189 ]. miR-203a-3p is evidenced to inhibit SMAD1 in HFSC of Sprague Dawley (SD) rats [ 190 ], which positively influences on hair cycle as SMADs are known to inhibit HF differentiation and hair growth [ 2 , 210 ].…”

Wnt/β-catenin Signaling Pathway Promotes Transdifferentiation from Fetal Skin Fibroblasts to Keratinocyte-like Cells

“…To explore the characteristics of KLCs after transdifferentiation, we compared KLCs with HFF-1 and KCs. From a molecular level, the Western blot results revealed that the epithelial marker protein Ecadherin [24] as well as KCs-labeled proteins, including CK5 [25], CK14 [26], CK19 [27,28] and Integrin beta1 [29] were expressed in KCs and KLCs, but not in HFF-1. Meanwhile, the HFF-1 marker protein Vimentin [22,23] was expressed in HFF-1, but not in KLCs or KCs (Figure 2a).…”

MIR31 May Mediate Multiple Biology Processes To Promote Vocal Fold Wound Healing

“…Vocal fold healing could take from two weeks to several months (Tateya et al, 2006;Ling et al, 2010;Gurtner et al, 2008). Multiple genes and proteins have been shown to play roles in the wound healing process (Luo et al, 2020;Spallotta et al, 2013). For instance, SMAD1 has been suggested as a therapeutic molecular target in skin wound healing that plays an active role in wound repair and regenerative medicine (Luo et al, 2020).…”

“…Multiple genes and proteins have been shown to play roles in the wound healing process (Luo et al, 2020;Spallotta et al, 2013). For instance, SMAD1 has been suggested as a therapeutic molecular target in skin wound healing that plays an active role in wound repair and regenerative medicine (Luo et al, 2020). Spallotta et al's study showed that HDAC2 inhibition could signi cantly enhance skin wound repair (Spallotta et al, 2013).…”