MiR‐205‐5p promotes lung cancer progression and is valuable for the diagnosis of lung cancer

Abstract

Background: MicroRNAs (miRNAs) function as potential diagnostic biomarkers in various cancers. This study aimed to evaluate the roles of miR-205-5p in lung cancer progression and diagnosis. Materials and Methods: MiR-205-5p was detected by quantitative real-time PCR. The effect of miR-205-5p on cell proliferation and metastasis was estimated by MTT and flow cytometry. The expression of TP53INP1 and related genes was analyzed by immunoblotting. The diagnostic value of miR-205-5p was analyzed using receiver operating characteristic (ROC) curve analysis, sensitivity, and specificity. Results: The miR-205-5p was increased in lung cancer tissues. MiR-205-5p mimics were promoted but its inhibitor suppressed cell proliferation and metastasis compared with control treatment in vitro and in vivo. By regulating the 3 0 untranslated region, miR-205-5p could negatively regulate TP53INP1 expression, which further inhibited the expression of RB1 and P21, but increased that of cyclinD1. Moreover, the serum miR-205-5p levels of patients with lung cancer were significantly higher than those of normal controls, and they were correlated with patients' gender, drinking status, and clinical stage. The area under the ROC curve of serum miR-205-5p in the diagnosis of non-small-cell lung cancer was 0.8250, respectively. The finding supported its possession of high diagnostic efficiency for lung cancer. Conclusions: MiR-205-5p promoted lung cancer cell proliferation and metastasis by negatively regulating the novel target TP53INP1, which further affected the expression of P21, RB1, and cyclin D1. Serum miR-205-5p is a novel and valuable biomarker for lung cancer diagnosis.

Integrating miRNAs and Bacterial DNA for Early Detection of Lung Cancer

“…Our deep sequencing analyses have shown that miR-205-5p exhibits a higher expression level in lung tumor tissues compared with noncaseous lung tissues and could provide a potential biomarker for NSCLC (Li et al, 2017; Ma et al, 2014; Xing et al, 2010). Upregulation of miR-205-5p contribute to lung cancer cell proliferation and metastasis in vitro and in vivo , by regulating functions of TP53INP1, RB1, and P21(Zhao et al, 2022). Aberrant miR-210-3p expression in body fluids could diagnose several types of malignancies, including lung cancer (Papaconstantinou et al, 2013).…”

Proliferation, Metastasis, and Radiosensitivity of Nasopharyngeal Carcinoma Cells in the Expression and Effect of Kiwifruit Extract through the Regulation of miR-205-5p

“…MiR-205-5p functioned as a tumor promoter in many cancers, including ovarian cancer [ 21 ] and lung cancer [ 22 ]. On the contrary, many studies showed that miR-205-5p was downregulated in thyroid cancer, and its upregulation suppressed cell proliferation and invasion [ 23 ].…”

“…Meanwhile, the survival fraction of NPC cells were decreased, and the SER ratio was increased after RAE treatment, confirming that RAE enhanced NPC cell radiosensitivity. MiR-205-5p functioned as a tumor promoter in many cancers, including ovarian cancer [21] and lung cancer [22]. On the contrary, many studies showed that miR-205-5p was downregulated in thyroid cancer, and its upregulation suppressed cell proliferation and invasion [23].…”

Identification of a dysregulated ceRNA network modulated by copy number variation‐driven lncRNAs in lung squamous cell carcinoma

“…MiR‐130b‐5p is known to accelerate cell migration and proliferation in gastric cancer (Chen et al, 2018). MiR‐205‐5p is a valuable diagnostic marker in lung cancer and a protective factor in gastric cancer, which suppresses angiogenesis in gastric cancer through downregulation of VEGFA and FGF1 (Zhang et al, 2021; Zhao et al, 2022). The results mentioned above exhibited that our regulatory network was closely related to the progression of cancer, and six key miRNAs were modulated by CNV‐driven lncRNAs, which may be novel biomarkers for LUSC.…”