PCL/collagen blends prepared by solution blow spinning as potential materials for skin regeneration

Abstract

Solution blow spinning (SBS), is used to prepare biocompatible fibrous materials based on poly-ε-caprolactone (PCL), modified with collagen. Materials with different compositions in terms of collagen are prepared. Structure, morphology, topography, wettability behavior, and cytotoxicity are studied in order to investigate the potentiality of these materials for medical applications in the field of tissue repairing and regeneration. Structure is studied by Fourier transformed infrared spectroscopy, morphology by scanning electron microscopy, topography by optical profilometry and wettability behavior by contact angle measurements. The addition of small amounts of collagen to PCL by SBS can induce important variations in the morphology and topography of the materials that, in turns, lead to changes in the wettability behavior and ability of HaCat cells adhesion and proliferation. The analysis of surface characteristics together with the use of a model based on mats constituted by cylinders disposed perpendicularly to each other point out that, under the compositions considered, the main factor leading to higher adhesion and proliferation of cells on the PCL/collagen is the presence of more available surface area.

Dressing systems based on chitosan as active transport platforms in the treatment of burnt skin: Mini‐review

“…In general, an ideal dressing or a skin regeneration system for treating wounds can be characterized based on criteria that include factors such as (1) providing and maintaining a moist environment (2) protecting the wound against secondary infections, acting as a bacterial barrier (3) allowing adequate gas exchange; (4) insulation against external agents; (5) proper management of excess exudate, allowing for non-traumatic removal; and (6) elasticity and biocompatibility. 38 Many compositions have been evaluated for these systems, including various natural 16,39 and synthetic polymeric matrices 40,41 along with a large array of therapeutic agents that mainly include the use of biomolecules, 16,42 biomimetic components, 40,43 natural, 44,45 and synthetic actives. 43,46 These components aim to, among other actions, reduce inflammatory conditions, provide antimicrobial activity or signal to specific pathways to aid in tissue regeneration.…”

“…43,46 These components aim to, among other actions, reduce inflammatory conditions, provide antimicrobial activity or signal to specific pathways to aid in tissue regeneration. 16,40,42,43,46 In addition, some systems also use micro or nanoparticles as a mechanical reinforcing agent or target local biological activities such as antimicrobial activity. 16,47,48 Among the many formulations in existence, systems based on chitosan stand out due to the biological properties presented by this polymeric matrix, which grant significant improvements in healing time, as will be discussed in the following topics.…”

“…Many compositions have been evaluated for these systems, including various natural 16,39 and synthetic polymeric matrices 40,41 along with a large array of therapeutic agents that mainly include the use of biomolecules, 16,42 biomimetic components, 40,43 natural, 44,45 and synthetic actives 43,46 . These components aim to, among other actions, reduce inflammatory conditions, provide antimicrobial activity or signal to specific pathways to aid in tissue regeneration 16,40,42,43,46 . In addition, some systems also use micro or nanoparticles as a mechanical reinforcing agent or target local biological activities such as antimicrobial activity 16,47,48 …”

Solution Blow Spinning (SBS): A Promising Spinning System for Submicron/Nanofibre Production

“…Food-grade gelatine nanofibres from pork skin gelatines (PGs) and fish skin gelatine are prepared by the SBS system [99]. Renewable polymers [100], such as starch, chitosan, cellulose [98], alginate, fibrinogen, fibrin, gelatine, and collagen need to be explored more and more because of their excellent performances in cell adhesion, proliferation [101], migration, differentiation, and characteristics analogous to those of ECM. However, many research works are supposed to be conducted to materialize fully the possibilities of the SBS spinning system for application in different fields.…”

Controlled release of thiram pesticide from polycaprolactone micro and nanofibrous mat matrix

“…Long-term implants and controlled drug release constitute the main applications of PCL. [10][11][12][13] PCL degrades very slowly (2 to 3 years) under in-vivo conditions. 14 Due to its semi-crystalline microstructure, PCL can be degraded selectively by microorganisms.…”