pH‐response of protein‐polysaccharide multilayers adsorbed on a flat gold surface: A surface plasmon resonance study

Abstract

Polysaccharide‐protein multilayers (PPMLs) consisting of bovine serum albumin (BSA) and chondroitin sulfate (CS) are assembled in acidic solution (pH 4.2) via layer‐by‐layer deposition method. The formation of PPMLs on gold surface and their responsiveness to pH change from 4.2 to 7 is investigated by Surface Plasmon Resonance Spectroscopy. The buildup of the multilayer at pH 4.2 exhibits non‐linear growth while the formation of the first layers is strongly affected by the physicochemical properties of the gold surface. Neutral solution (pH 7) affects the interactions between the biopolymers and results in a partially disassemble (disintegration) of the multilayer film. On one hand, the single pair of layers, BSA‐CS and the double pair of layers, (BSA‐CS)2, assemblies are stable in neutral pH, a result that will be of interest for biomedical applications. On the other hand, multilayer films consisting of more than four layers that is (BSA‐CS)2<n<5, disintegrated down to the 4‐layered structure by changing pH to neutral, a fact that renders the (BSA‐CS)n assembly useful in the field of drug and protein delivery. The residual mass after the disintegration of the assembly never falls below the mass of four layers. The disintegrated multilayer film can be reconstructed and disassembled repeatedly, simply by cycling the pH value.