<scp>PM</scp> 7/141 (1) <i>Philaenus spumarius, Philaenus italosignus</i> and <i>Neophilaenus campestris</i>

doi:10.1111/epp.12610

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…Upon collection, the insects were carefully transferred to a plastic bag, and euthanasia was carried out by injecting ether. Subsequently, they were transported in a cooler box to the laboratory and immediately identified and separated by sex under a binocular stereomicroscope (Leica EZ4), according to the morphological descriptions provided by Germain et al (2020) [16]. The insects were then subjected to a sterilization process previously optimized by us.…”

Section: Philaenus Spumarius Collection and Processingmentioning

confidence: 99%

Diversity, Composition, and Specificity of the Philaenus spumarius Bacteriome

Cameirão,

Costa,

Rufino

et al. 2024

Microorganisms

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Philaenus spumarius (Linnaeus, 1758) (Hemiptera, Aphrophoridae) was recently classified as a pest due to its ability to act as a vector of the phytopathogen Xylella fastidiosa. This insect has been reported to harbour several symbiotic bacteria that play essential roles in P. spumarius health and fitness. However, the factors driving bacterial assemblages remain largely unexplored. Here, the bacteriome associated with different organs (head, abdomen, and genitalia) of males and females of P. spumarius was characterized using culturally dependent and independent methods and compared in terms of diversity and composition. The bacteriome of P. spumarius is enriched in Proteobacteria, Bacteroidota, and Actinobacteria phyla, as well as in Candidatus Sulcia and Cutibacterium genera. The most frequent isolates were Curtobacterium, Pseudomonas, and Rhizobiaceae sp.1. Males display a more diverse bacterial community than females, but no differences in diversity were found in distinct organs. However, the organ shapes the bacteriome structure more than sex, with the Microbacteriaceae family revealing a high level of organ specificity and the Blattabacteriaceae family showing a high level of sex specificity. Several symbiotic bacterial genera were identified in P. spumarius for the first time, including Rhodococcus, Citrobacter, Halomonas, Streptomyces, and Providencia. Differences in the bacterial composition within P. spumarius organs and sexes suggest an adaptation of bacteria to particular insect tissues, potentially shaped by their significance in the life and overall fitness of P. spumarius. Although more research on the bacteria of P. spumarius interactions is needed, such knowledge could help to develop specific bacterial-based insect management strategies.

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Section: Philaenus Spumarius Collection and Processingmentioning

confidence: 99%