PM 7/29 (3) Tilletia indica

Abstract

Diagnostics Diagnostic PM 7/29 (3) Tilletia indica Specific scope This Standard describes a diagnostic protocol for Tilletia indica. 1 It should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols. Specific approval and amendment This Standard was originally developed under the EU DIAGPRO Project (SMT 4-CT98-2252) by a partnership of contractor laboratories and interlaboratory comparison in European countries. First approved as an EPPO Standard in 2003-09. First revision approved in 2007-09. Second revision approved on 2017-11. Although this EPPO Diagnostic Standard differs in terms of format it is in general consistent with the content of the IPPC Standard adopted in 2014 on Tilletia indica (Annex 4 to ISPM 27) with the following exceptions.(1) In the EPPO region, as the pest is not present, a higher confidence in the results is required, a sieve wash test should be carried out (optional in the IPPC protocol). (2) When fewer than 10 teliospores are found the options should allow testing the (<10) teliospores with conventional or real-time PCR (this was not an option in the IPPC protocol flow chart, although it was stated that direct real-time PCR could be used on individual teliospores in the text). (3) The method for extracting teliospores from untreated seed or grain by sizeselective sieving is slightly different based on the experience in the region (European Union test performance study). The EPPO Diagnostic Standard also includes a test for a direct real-time PCR for use on pellets (developed in 2016). Some additional information on methods for morphological identification, from the former version of the EPPO Standard, which are not in the IPPC protocol are included in this protocol in Appendix 3 as they were considered useful by the members of the Panel on Diagnostics in Mycology.