PPARG agonist pioglitazone influences diurnal kidney medulla mRNA expression of core clock, inflammation‐, and metabolism‐related genes disrupted by reverse feeding in mice

Abstract

This study examined the influence of PPARG activation by pioglitazone (PG) on the mRNA of core clock, inflammation‐ and metabolism‐related genes in the mouse kidney medulla as well as urinary sodium/potassium excretion rhythms disrupted by reverse feeding. Mice were assigned to daytime feeding and nighttime feeding groups. PG 20 mg/kg was administered at 7 am or 7 pm . On day 8 of the feeding intervention, mice were killed at noon and midnight. Kidney medulla expression of Arntl, Clock, Nr1d1, Cry1, Cry2, Per1, Per2, Nfe2l2, Pparg , and Scnn1g was determined by qRT PCR. We measured urinary K + , Na + , urine volume, food, and H 2 O intake. The reverse feeding uncoupled the peripheral clock gene rhythm in mouse kidney tissues. It was accompanied by a decreased expression of Nfe2l2 and Pparg as well as an increased expression of Rela and Scnn1g. These changes in gene expressions concurred with an increase in urinary Na + , K + , water excretion, microcirculation disorders, and cell loss, especially in distal tubules. PG induced the restoration of diurnal core clock gene expression as well as Nfe2l2, Pparg , Scnn1g mRNA, and decreased Rela expressions, stimulating Na + reabsorption and inhibiting K + excretion. PG intake at 7 pm was more effective than at 7 am .

PPARG stimulation restored lung mRNA expression of core clock, inflammation‐ and metabolism‐related genes disrupted by reversed feeding in male mice

“…We investigated the ability of PZ to influence the mRNAs level of core clock genes and inflammationand metabolism-related gene disturbance by RF in mouse lungs. The influence of PZ and rosiglitazone on the clock gene expression was shown recently for mouse liver and kidney tissue (Fedchenko et al, 2022;Izmailova et al, 2022;Ribas-Latre et al, 2019). New data were obtained about the role of PPARs and PPARG in lung chronobiology and chronotherapy (Nosal et al, 2020;Paudel et al, 2021;Sundar et al, 2018).…”

“…RF might be an effective rodent model for the investigation of the time‐of‐day eating variations. This experimental model breaks the peripheral molecular clock in the mouse heart, liver, and kidney (Fedchenko et al, 2022 ; Izmailova et al, 2022 ; Oyama et al, 2014 ; Yamamura et al, 2010 ).…”

“…By using the same model of RF and PZ administration in male mice, we can compare the effects of such interventions in the three metabolic active organs—liver, kidney, and lung (Fedchenko et al, 2022 ; Izmailova et al, 2022 ).…”

Вплив Модуляторів Циркадіанного Ритму На Екскреторну Та Натрійрегулювальну Функцію Нирок Щурів За Умов Гострого Десинхронозу Та Ліпополісахарид-Індукованої Системної Запальної Відповіді

“…Гострий десинхроноз (ГД) триває в середньому 10-15 днів [9]. Прикладом такого стану може бути неузгодженість між ендогенними цикрадіанними ритмами внутрішніх годинників і зовнішнім середовищем, що призводить до порушень функціонального стану нирок [10] та може сприяти розвитку ХХН [11].…”