<scp>RASSF</scp>
            1A controls tissue stiffness and cancer stem‐like cells in lung adenocarcinoma

Paňková, Daniela; Jiang, Yanyan; Chatzifrangkeskou, Maria; Vendrell, Iolanda; Buzzelli, Jon N.; Ryan, Anderson J.; Brown, Cameron; O’Neill, Eric

doi:10.15252/embj.2018100532

Cited by 101 publications

(88 citation statements)

References 106 publications

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“…Further studies comparing CSC-related genes of cells cultured in 2D and Col-I-coated 2D are then needed. High Col-I density modestly impacts Nanog nuclear levels in colorectal carcinoma cells in comparison to low Col-I density (Pankova et al, 2019). As for MCF-7, our results are in line with Sox2 and Bmi1 transcripts, but not nuclear ERa, being increased in Col-I-enriched ER + murine breast tumors (Shea et al, 2018).…”

Section: Discussionsupporting

confidence: 84%

High type I collagen density fails to increase breast cancer stem cell phenotype

Valadão

Ralph

Bordeleau

et al. 2020

PeerJ

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Breast cancer is a highly frequent and lethal malignancy which metastasis and relapse frequently associates with the existence of breast cancer stem cells (CSCs). CSCs are undifferentiated, aggressive and highly resistant to therapy, with traits modulated by microenvironmental cells and the extracellular matrix (ECM), a biologically complex and dynamic structure composed mainly by type I collagen (Col-I). Col-I enrichment in the tumor-associated ECM leads to microenvironment stiffness and higher tumor aggressiveness and metastatic potential. While Col-I is also known to induce tumor stemness, it is unknown if such effect is dependent of Col-I density. To answer this question, we evaluated the stemness phenotype of MDA-MB-231 and MCF-7 human breast cancer cells cultured within gels of varying Col-I densities. High Col-I density increased CD44+CD24− breast cancer stem cell (BCSC) immunophenotype but failed to potentiate Col-I fiber alignment, cell self-renewal and clonogenicity in MDA-MB-231 cells. In MCF-7 cells, high Col-I density decreased total levels of variant CD44 (CD44v). Common to both cell types, high Col-I density induced neither markers related to CSC nor those related with mechanically-induced cell response. We conclude that high Col-I density per se is not sufficient to fully develop the BCSC phenotype.

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Section: Discussionsupporting

confidence: 84%

High type I collagen density fails to increase breast cancer stem cell phenotype

Valadão

Ralph

Bordeleau

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…Further studies comparing CSC-related genes of cells cultured in 2D and Col-I-coated 2D are then needed. High Col-I density modestly impacts Nanog nuclear levels in colorectal carcinoma cells in comparison to low Col-I density (Pankova et al, 2019). As for MCF-7, our results are in line with Sox2 and Bmi1 transcripts, but not nuclear ERα, being increased in Col-I-enriched ER + murine breast tumors (Shea et al, 2018).…”

Section: Discussionsupporting

confidence: 84%

Peer Review #1 of "High type I collagen density fails to increase breast cancer stem cell phenotype (v0.1)"

2020

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Breast cancer is a highly frequent and lethal malignancy which metastasis and relapse frequently associates with the existence of breast cancer stem cells (CSCs). CSCs are undifferentiated, aggressive and highly resistant to therapy, with traits modulated by microenvironmental cells and the extracellular matrix (ECM), a biologically complex and dynamic structure composed mainly by type I collagen (Col-I). Col-I enrichment in the tumor-associated ECM leads to microenvironment stiffness and higher tumor aggressiveness and metastatic potential. While Col-I is also known to induce tumor stemness, it is unknown if such effect is dependent of Col-I density. To answer this question, we evaluated the stemness phenotype of MDA-MB-231 and MCF-7 human breast cancer cells cultured within gels of varying Col-I densities. High Col-I density increased CD44 + CD24-breast cancer stem cell (BCSC) immunophenotype but failed to potentiate Col-I fiber alignment, cell self-renewal and clonogenicity in MDA-MB-231 cells. In MCF-7 cells, high Col-I density decreased total levels of variant CD44 (CD44v). Common to both cell types, high Col-I density induced neither markers related to CSC nor those related with mechanically-induced cell response. We conclude that high Col-I density per se is not sufficient to fully develop the BCSC phenotype.

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“…H&E staining is the gold standard for tumor diagnosis. With the development of biomechanics in the past two decades [46,47], the mechanical properties of tissues need to be investigated based on biomedical and physical parameters. In this study, we have assayed the changes in mechanical properties at the micro-nanometer level using AFM and determined the association between the TNM grade, metastasis, and stiffness of tumor samples.…”

Section: Discussionmentioning

confidence: 99%

Tissue mechanics and expression of TROP2 in oral squamous cell carcinoma with varying differentiation

Zhang

Gao

et al. 2020

BMC Cancer

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Background: Trophoblast cell surface antigen 2 (TROP2) is overexpressed in many squamous cell carcinomas and promotes tumor development and invasion. The association between TROP2 expression and occurrence and development of oral squamous cell carcinoma (OSCC) remains to be understood. Methods: We investigated the role of TROP2 in OSCC patients using a combination of biophysical approaches. A total of 108 OSCC patient specimens with varying degrees of differentiation were subjected to hematoxylin and eosin staining, immunohistochemistry, Kaplan-Meier survival curve analysis, and atomic force microscopy to analyze TROP2 expression, morphology, and mechanical properties of OSCC tissues. Results: TROP2 was overexpressed in 34% of poorly differentiated OSCC samples. High levels of TROP2 were associated with 10.2% survival rate lower than 45.4% and patient age (odds ratio [OR] = 0.437, P = 0.039, 95% confidence interval [CI, 0.198-0.966]), tumor size (OR = 13.148, P = 0.000, 95% CI [5.060-34.168]), and TNM stage (OR = 0.141, P = 0.000, 95% CI [0.082-0.244]). Average surface roughness of low, medium, and highly differentiated OSCC tissues were 448.9 ± 54.8, 792.7 ± 83.6, and 993.0 ± 104.3 nm, respectively. The Pearson coefficient revealed a negative association between tumor stiffness and TROP2 expression (r = − 0.84, P < 0.01). Conclusion: Overexpression of TROP2 negatively associated with patient survival, degree of tumor differentiation, and tissue mechanics. Taken together, our findings demonstrated that TROP2 may be an indicator of OSCC differentiation leading to the altered mechanical properties of OSCC tissues.

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RASSF 1A controls tissue stiffness and cancer stem‐like cells in lung adenocarcinoma

Cited by 101 publications

References 106 publications

High type I collagen density fails to increase breast cancer stem cell phenotype

High type I collagen density fails to increase breast cancer stem cell phenotype

Peer Review #1 of "High type I collagen density fails to increase breast cancer stem cell phenotype (v0.1)"

Tissue mechanics and expression of TROP2 in oral squamous cell carcinoma with varying differentiation

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