<scp>RNA</scp>‐binding protein <scp>DHX9</scp> promotes glioma growth and tumor‐associated macrophages infiltration via <scp>TCF12</scp>

Liu, Liang; Zhou, Xuelan; Cheng, Shan; Ge, Yuyuan; Chen, Baomin; Song, Jia; Li, Haoran; Li, Suwen; Yuan, Jiaqi; Wu, Anyi; Liu, Xinglei; Huang, Shilu; Xu, Zhipeng; Dong, Jun

doi:10.1111/cns.14031

Cited by 24 publications

(15 citation statements)

References 37 publications

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“…The expression of the RNA helicase in various tissues is different, and the expression in different varieties of sweetpotato is also quite different. This also shows that the RNA helicase is related to growth and development (Tyagi et al, 2020;Liu et al, 2023). Some the RNA helicase genes were highly communicated in stems and roots of sweetpotato, such as IbDExDH25, IbDEAH42, IbDExDH47, and IbDExDH96.…”

Section: Rna Helicase Family Of Different Sweet Potato Cultivarsmentioning

confidence: 70%

Genome-wide Systematic Survey and Analysis of the RNA Helicase Gene Family and their Response to Abiotic Stress in Sweetpotato

Mu,

Zheng,

Zhao

et al. 2023

Preprint

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RNA helicase is a large family that plays a significant role in plant evolution and in the abiotic stress response. Sweetpotato is one of the majority essential foods in the world, and their yields are often contrived by environmental stresses. Previously, the RNA helicase gene family has not been described in sweetpotato, hence we went a comprehensive genome-wide examination of the sweetpotato RNA helicase family, containing chromosome distribution, promoter elements, and motif compositions. All of 300 RNA helicase genes were divided into three subfamilies, including IbDEAD, IbDEAH and IbDExDH, and they are unevenly scattered across 15 chromosomes of the sweetpotato. Furthermore, tandem replication and segmental replication events played a key role in the amplification of sweetpotato RNA helicase genes. The collinear relationship amidst sweetpotato RNA helicase genes and 8 other related homologous genes in plants was deeply explored, which supplied a reliable basis for studying the development of sweetpotato RNA helicase gene family. RNA-seq examination and qRT-PCR recognition showed that the expression of eight RNA helicase genes was significantly diverse under four abiotic stresses (cold, drought, heat, salt). At the same time, the expression of these RNA helicases in different tissues of the 10 sweetpotato varieties also differed significantly. The promoters of the RNA helicase genes contain a great deal of cis-acting elements related to temperature, hormone and light response. The results indicated that sweetpotato RNA helicase genes played a key role in development or the abiotic stress response.

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Section: Rna Helicase Family Of Different Sweet Potato Cultivarsmentioning

confidence: 70%

Genome-wide Systematic Survey and Analysis of the RNA Helicase Gene Family and their Response to Abiotic Stress in Sweetpotato

Mu,

Zheng,

Zhao

et al. 2023

Preprint

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“…Targeting M2 TAMs in TME is considered as an potential effective therapeutic strategy against cancer 27 . Our previous studies have found that GBM could promote glioma‐associated macrophage infiltration and M2 polarization 28 . Exosomes are membrane‐enclosed extracellular vesicles carrying biomolecules that include proteins and nucleic acids, especially miRNAs 16 .…”

Section: Discussionmentioning

confidence: 99%

“…27 Our previous studies have found that GBM could promote glioma-associated macrophage infiltration and M2 polarization. 28 Exosomes are membrane-enclosed extracellular vesicles carrying biomolecules that include proteins and nucleic acids, especially miRNAs. 16 In the current studies, we explored the effects and underlying mechanisms of cosstalk between GSCs and macrophages on M2 polarization, which disclosed that GSCs-derived exosomal miR-6733-5p can induce M2 macrophage polarization, and polarized M2 macrophages promote F I G U R E 2 Glioblastoma stem cells (GSCs)-exo induces M2 macrophage polarization.…”

Section: Discussionmentioning

confidence: 99%

Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically

Huang

Liu

et al. 2023

CNS Neurosci Ther

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AimExosomal miRNAs derived from glioblastoma stem cells (GSCs) are important mediators of immunosuppressive microenvironment formation in glioblastoma multiform (GBM), especially in M2‐like polarization of tumor‐associated macrophages (TAMs). However, the exact mechanisms by which GSCs‐derived exosomes (GSCs‐exo) facilitate the remodeling of the immunosuppressive microenvironment of GBM have not been elucidated.MethodsTransmission electron microscopy (TME) and nanoparticle tracking analysis (NTA) were applied to verify the existence of GSCs‐derived exosomes. Sphere formation assays, flow cytometry, and tumor xenograft transplantation assays were performed to identify the exact roles of exosomal miR‐6733‐5p. Then, the mechanisms of miR‐6733‐5p and its downstream target gene regulating crosstalk between GSCs cells and M2 macrophages were further investigated.ResultsGSCs‐derived exosomal miR‐6733‐5p induce macrophage M2 polarization of TAMs by positively targeting IGF2BP3 to activate the AKT signaling pathway, which further facilitates the self‐renewal and stemness of GSCs.ConclusionGSCs secrete miR‐6733‐5p‐rich exosomes to induce M2‐like polarization of macrophages, as well as enhance GSCs stemness and promote malignant behaviors of GBM through IGF2BP3 activated AKT pathway. Targeting GSCs exosomal miR‐6733‐5p may provide a potential new strategy against GBM.

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“…Ndufs1 (NADH:Ubiquinone Oxidoreductase Core Subunit S1; UniProt P28331) is involved in the adaptability of tumor cells to their environment, particularly at low pH 64 . Furthermore, Dhx9 , Dusp12 , Fhl1 , and Ifitm1 are each correlated with the invasion of tumor‐infiltrating immune cells into the microenvironment 59,65–67 . In contrast, Pja2 (Praja Ring Finger Ubiquitin Ligase 2; UniProt O43164) promotes anti‐cancer M1 macrophage polarization 68 .…”

Section: Discussionmentioning

confidence: 99%

An engineered niche delineates metastatic potential of breast cancer

Orbach,

DeVaull,

Bealer

et al. 2023

Bioengineering & Transla Med

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Metastatic breast cancer is often not diagnosed until secondary tumors have become macroscopically visible and millions of tumor cells have invaded distant tissues. Yet, metastasis is initiated by a cascade of events leading to formation of the pre‐metastatic niche, which can precede tumor formation by a matter of years. We aimed to distinguish the potential for metastatic disease from nonmetastatic disease at early times in triple‐negative breast cancer using sister cell lines 4T1 (metastatic), 4T07 (invasive, nonmetastatic), and 67NR (nonmetastatic). We used a porous, polycaprolactone scaffold, that serves as an engineered metastatic niche, to identify metastatic disease through the characteristics of the microenvironment. Analysis of the immune cell composition at the scaffold was able to distinguish noninvasive 67NR tumor‐bearing mice from 4T07 and 4T1 tumor‐bearing mice but could not delineate metastatic potential between the two invasive cell lines. Gene expression in the scaffolds correlated with the up‐regulation of cancer hallmarks (e.g., angiogenesis, hypoxia) in the 4T1 mice relative to 4T07 mice. We developed a 9‐gene signature (Dhx9, Dusp12, Fth1, Ifitm1, Ndufs1, Pja2, Slc1a3, Soga1, Spon2) that successfully distinguished 4T1 disease from 67NR or 4T07 disease throughout metastatic progression. Furthermore, this signature proved highly effective at distinguishing diseased lungs in publicly available datasets of mouse models of metastatic breast cancer and in human models of lung cancer. The early and accurate detection of metastatic disease that could lead to early treatment has the potential to improve patient outcomes and quality of life.

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RNA‐binding protein DHX9 promotes glioma growth and tumor‐associated macrophages infiltration via TCF12

Cited by 24 publications

References 37 publications

Genome-wide Systematic Survey and Analysis of the RNA Helicase Gene Family and their Response to Abiotic Stress in Sweetpotato

Genome-wide Systematic Survey and Analysis of the RNA Helicase Gene Family and their Response to Abiotic Stress in Sweetpotato

Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically

An engineered niche delineates metastatic potential of breast cancer

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