<scp>RNA</scp> interference in shrimp and potential applications in aquaculture

Nguyen, Dung Viet; Christiaens, Olivier; Bossier, Peter; Smagghe, Guy

doi:10.1111/raq.12187

Cited by 27 publications

(15 citation statements)

References 101 publications

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“…Evidence of such a phenomenon was reported in arthropods, specifically in insects [for example, the African malaria mosquito ( 23 )], and as well was observed in shrimp as detailed in this study. Transcriptional silencing generally occurs through long-dsRNA (>200 bp), which is processed into short interfering RNAs (siRNA of 20–30 bp) by endogenous Dicer (an RNase-III-like enzyme) in cells; the resultant siRNA represses transcription of the target gene ( 24 , 25 ). Therefore, the endogenously produced siRNA may disrupt the expression of homologous genes with short-matched sequences.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, there were no large differences in oocyte development among the various treatments, but there did seem to be more early-stage endogenous vitellogenic oocytes in the mixed-dsRNA group at 10 days as shown in Figure 4E-1 . On the other hand, RNAi techniques using dsRNA have been used to increase immunity in shrimps; injection of dsRNA for non-specific genes improved the survival of diseased shrimp affected with white spot syndrome virus ( 25 , 30 ). In our case, injection of GFP-dsRNA may have eventually improved shrimp health conditions in L. vannamei .…”

Section: Discussionmentioning

confidence: 99%

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Assessment of the Effects of Double-Stranded RNAs Corresponding to Multiple Vitellogenesis-Inhibiting Hormone Subtype I Peptides in Subadult Female Whiteleg Shrimp, Litopenaeus vannamei

2021

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Vitellogenesis-inhibiting hormone (VIH) negatively regulates reproduction in shrimp and other decapod crustaceans. In order to assess the effects of transcriptional silencing by multiple VIH subtype I sinus gland peptides (SGPs) on ovarian maturation in female whiteleg shrimp, Litopenaeus vannamei, we synthesized five dsRNAs targeting Liv-SGP-A, −B, −C, −F, and −G and injected them into subadults. The following treatments were employed: sgpG-dsRNA (targeting Liv-SGP-G), sgpC-dsRNA (targeting Liv-SGP-C), and mixed-dsRNA (targeting Liv-SGP-A, −B, and −F). The expression of Liv-SGP-G in eyestalks was significantly decreased at 10, 20, and 30 days after the injection of sgpG-dsRNA In addition, it was significantly decreased at 10 and 30 days after the injection of mixed-dsRNA. The expression of vitellogenin (Vg) gene expression in the ovaries, and concentrations of Vg protein in the hemolymph, were not changed by the administration of any dsRNA treatment (the ovaries remained immature in all treated individuals and contained mostly oogonia and previtellogenic oocytes). Although the administration of dsRNAs corresponding to multiple VIHs did not promote ovarian maturation, this is the first report of the co-transcriptional repression of Liv-SGP-G by the injection of dsRNA for homologous genes (Liv-SGP-A, −B, and −F). These results indicate that subadults can respond to the techniques of transcriptional silencing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessment of the Effects of Double-Stranded RNAs Corresponding to Multiple Vitellogenesis-Inhibiting Hormone Subtype I Peptides in Subadult Female Whiteleg Shrimp, Litopenaeus vannamei

2021

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“…The presence of the microRNA biogenesis pathway in penaeid shrimp has been described (Nguyen et al 2016), indicating the expression of key components from this biological pathway. In Penaeus monodon, DNA constructs that expressed long-hairpin RNA constitutively against two White Spot Syndrome Virus (WSSV) envelop genes conferred protection upon pathogen infection, which indirectly suggest a functional microRNA pathway (Krishnan et al 2009).…”

Section: Microrna Biogenesis In Penaeid Shrimpmentioning

confidence: 99%

The role of shrimp microRNAs in immune response and beyond

González-Duarte¹,

García‐Carreño²

2018

Reviews in Aquaculture

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MicroRNAs are a class of small non‐coding RNAs that regulate gene expression at post‐transcriptional level. Regulatory RNAs were discovered in the nematode Caenorhabditis elegans, since then, their biological functions have been studied on different animals, including marine organisms. Several species of penaeid shrimp are important in ecology and fishing, including aquaculture. Overcrowding in aquaculture calls for infectious diseases, which threaten the development of shrimp aquaculture worldwide. Given that microRNAs play crucial regulatory roles in a wide variety of biological processes, there is a scientific interest to understand their contribution on shrimp physiology and pathology, specifically during immune and stress response. Increasing evidence has shown that the expression of microRNAs is affected during virus or bacterial infection and upon stress in shrimp. This information provides valuable insights for a better understanding of shrimp biology by means of microRNA regulation to bacterial and viral diseases.

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“…The experimental application of RNAi for gene silencing in arthropods including Acari exploits the siRNA pathway, which is activated mainly via the exogenous administration of specific synthetic dsRNAs [12,13,[23][24][25]. In principle, three molecular processes are expected to define the success of gene silencing: the uptake of dsRNA by the target cell, the amplification of the siRNAs and the transport of the latter to distant cells to induce gene silencing throughout the treated organism, a phenomenon referred to as systemic RNAi [23].…”

Section: Introductionmentioning

confidence: 99%

A genome-wide screening for RNAi pathway proteins in Acari

2020

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Background RNA interference (RNAi) is a highly conserved, sequence-specific gene silencing mechanism present in Eukaryotes. Three RNAi pathways are known, namely micro-RNA (miRNA), piwi-interacting RNA (piRNA) and short interfering RNA (siRNA). However, little knowledge exists about the proteins involved in these pathways in Acari. Moreover, variable successes has been obtained in gene knockdown via siRNA pathway in their functional genomics and management. We hypothesized that the clue may be in the variability of the composition and the efficacy of siRNA machinery among Acari. Results Both comparative genomic analyses and domain annotation suggest that all the analyzed species have homologs of putative core proteins that mediate cleaving of targeted genes via the three RNAi pathways. We identified putative homologs of Caenorhabditis elegans RNA-dependent RNA polymerase (RdRP) protein in all species though no secondary Argonaute homologs that operate with this protein in siRNA amplification mechanism were found, suggesting that the siRNA amplification mechanism present in Acari may be distinct from that described in C. elegans. Moreover, the genomes of these species do not encode homologs of C. elegans systemic RNAi defective-1 (Sid-1) protein that mediate silencing of the mRNA target throughout the treated organisms suggesting that the phenomena of systemic RNAi that has been reported in some Acari species probably occur through a different mechanism. However, homologs of putative RNAi spreading defective-3 (Rsd-3) protein and scavenger receptors namely Eater and SR-CI that mediate endocytosis cellular update of dsRNA in C. elegans and Drosophila melanogaster were found in Acari genomes. This result suggests that cellular dsRNA uptake in Acari is endocytosis-dependent. Detailed phylogenetic analyses of core RNAi pathway proteins in the studied species revealed that their evolution is compatible with the proposed monophyletic evolution of this group. Conclusions Our analyses have revealed the potential activity of all three pathways in Acari. Still, much experimental work remains to be done to confirm the mechanisms behind these pathways in particular those that govern systemic/parental RNAi and siRNA amplification in Acari. Disclosure of these mechanisms will facilitate the development of new and specific management tools for the harmful species and enrichment of the beneficial species.

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RNA interference in shrimp and potential applications in aquaculture

Cited by 27 publications

References 101 publications

Assessment of the Effects of Double-Stranded RNAs Corresponding to Multiple Vitellogenesis-Inhibiting Hormone Subtype I Peptides in Subadult Female Whiteleg Shrimp, Litopenaeus vannamei

Assessment of the Effects of Double-Stranded RNAs Corresponding to Multiple Vitellogenesis-Inhibiting Hormone Subtype I Peptides in Subadult Female Whiteleg Shrimp, Litopenaeus vannamei

The role of shrimp microRNAs in immune response and beyond

A genome-wide screening for RNAi pathway proteins in Acari

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