<scp>RNAi</scp>‐mediated silencing of ferritin genes in the brown planthopper <scp><i>Nilaparvata lugens</i></scp> affects survival, growth and female fecundity

Shen, Yan; Chen, Yuan-Zhi; Zhang, Chuan‐Xi

doi:10.1002/ps.6026

Cited by 14 publications

(13 citation statements)

References 63 publications

(131 reference statements)

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“…Knockdown of ferritins in H. flava through RNAi led to a lower ratio of total weight of eggs laid to engorged body weight, and the eggs displayed abnormal morphologies [19]. In Nilaparvata lugens, silencing of ferritin1 heavy chain and ferritin2 light chain through RNAi led to undeveloped ovaries and severely inhibited oocyte growth [22]. In the present study, the expression of HrFer2 was highest in the midgut of H. rufipes, which might explain the significant effect of HrFer2 knockdown on the feeding duration of this tick.…”

Section: Discussionmentioning

confidence: 99%

The molecular and functional characterization of ferritins in the hard tick Hyalomma rufipes

Gao

Zheng²,

Wang³

et al. 2022

Parasites Vectors

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Background The protein ferritin, which plays an important role in the maintenance of iron homeostasis, is indispensable for iron detoxification, resistance to oxidative stress and innate immunity. Ticks, which are obligate blood-sucking ectoparasites, have to deal with a large amount of iron when they take a blood meal. Methods Sequence analysis was undertaken using bioinformatics. A recombinant (r) expression vector, rferritin, was constructed for a prokaryotic expression system. A quantitative polymerase chain reaction platform was used to detect the spatial and temporal expression patterns of target genes and their responses to a low temperature environment. Knockdown of the ferritin genes through RNA interference was used to analyze their effects on physiological parameters of ticks. Results Two ferritin genes, HrFer1 and HrFer2, were cloned from the tick Hyalomma rufipes. Their open reading frames are 519 base pairs (bp) and 573 bp in length, and number of coding amino acids 170 and 190, respectively. The phylogenetic tree showed that HrFer1 and HrFer2 have a close evolutionary relationship with the H subunit of ferritin. In vitro experiments showed that rHrFer1 and rHrFer2 had concentration-dependent iron chelating activity. The relative expression of the two ferritin genes was higher in the ovary and midgut of H. rufipes. RNA interference results demonstrated that HrFer1 and HrFer2 expression had a significant effect on engorged body weight, number of eggs laid, and mortality of H. rufipes, and that HrFer2 also had a significant effect on feeding duration. Furthermore, the relative expression of ferritin decreased significantly in a low temperature environment, suggesting that HrFer1 and HrFer2 play a regulatory role in the cold stress response of H. rufipes. Conclusions The results of the present study improve our understanding of the involvement of ferritins in tick blood-feeding. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

The molecular and functional characterization of ferritins in the hard tick Hyalomma rufipes

Gao

Zheng²,

Wang³

et al. 2022

Parasites Vectors

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“…RNA interference (RNAi)‐mediated pest management has been proven to be efficient in various species, such as Coleopteran 6 and Hemipteran pests, 7 and the construction of transgenic plants that mediate pest‐target RNAi is a promising method to control insect pests 8,9 . As proven in previous studies, efficient RNAi effects can be achieved in BPH based on both microinjection of double‐stranded RNA (dsRNA) into the hemolymph and oral ingestion of dsRNA 10–12 . At present, there are far fewer studies regarding genes involved in killing eggs than those concerning genes involved in disrupting nymphs or adult growth and development, and the application of ovicidal target genes is lacking 13 …”

Section: Introductionmentioning

confidence: 99%

“…8,9 As proven in previous studies, efficient RNAi effects can be achieved in BPH based on both microinjection of double-stranded RNA (dsRNA) into the hemolymph and oral ingestion of dsRNA. [10][11][12] At present, there are far fewer studies regarding genes involved in killing eggs than those concerning genes involved in disrupting nymphs or adult growth and development, and the application of ovicidal target genes is lacking. 13 Eggshells play an important role in the survival of insect eggs, including providing protection against risky external environments, controlling the exchange of air and water and providing micropylar apparatuses for spermatozoon entrance.…”

Section: Introductionmentioning

confidence: 99%

RNAi‐mediated silencing of an egg‐specific gene Nllet1 results in hatch failure in the brown planthopper

Wang

Ren

et al. 2022

Pest Management Science

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Background: The brown planthopper (BPH) is one of the most destructive agricultural pests in Asia. RNA interference (RNAi)mediated pest management has been under development for years, and the selection of appropriate target genes is important for pest-targeted RNAi. C-type lectins (CTLs) are a class of genes that perform a variety of functions, such as the regulation of growth and development.Results: A CTL-S protein named Nllet1, containing a single calcium ion (Ca 2+ )-dependent carbohydrate-binding domain (CRD) with a conserved triplet motif QPD was identified and functionally characterized in BPH. Expression profiles at both the transcriptional and translational levels show that Nllet1 accumulates during the serosal cuticle (SC) formation period. Immunofluorescence and immunogold labeling further demonstrated that Nllet1 is located in the serosal endocuticle (en-SC). Maternal RNAi-mediated silencing of Nllet1 disrupted the SC structure, accompanied by a loss of the outward barrier and 100% embryo mortality. Injection of 10 ng dsNllet1 or dsNllet1' per female adult BPH resulted in a total failure of egg hatching. Conclusion:Nllet1 is essential for SC formation and embryonic development in BPH, which helps us understand the important roles of CTL-Ss. Additionally, BPH eggs show high sensitivity to the depletion of Nllet1. This study indicates that Nllet1 is a promising candidate gene that can be used to develop RNAi-based control strategies at the BPH egg stage, and it can also be used as a target for developing novel ovicides.

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“…33,34 In N. lugens, knockdown of NlFer1 or NlFer2 led to retarded growth and 100% mortality in young nymphs. 35 In Locusta migratoria, silencing of nuclear export factor 1 (NXF1) resulted in the total hemocyte levels being reduced by 97%, leading to 93% insect mortality. 36 Alternatively, RNAi provides a method that makes it more difficult for pests to develop resistance, that is, combinatorial RNAi of multiple genes, which avoids the effects of dose compensation.…”

Section: Introductionmentioning

confidence: 99%

“…The application of RNAi was accomplished in various pest management strategies, such as in reducing pest resistance to pesticides or impairing the developmental processes of pests 33,34 . In N. lugens , knockdown of NlFer1 or NlFer2 led to retarded growth and 100% mortality in young nymphs 35 . In Locusta migratoria , silencing of nuclear export factor 1 ( NXF1 ) resulted in the total hemocyte levels being reduced by 97%, leading to 93% insect mortality 36 .…”

Section: Introductionmentioning

confidence: 99%

Silencing of the BtTPS genes by transgenic plant‐mediated RNAi to control Bemisia tabaciMED

Chen

Yang

et al. 2021

Pest Management Science

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Background: Whitefly (Bemisia tabaci) is a typical pest that causes severe damage to hundreds of agricultural crops. The trehalose-6-phosphate synthase (TPS) genes, as the key genes in the insect trehalose synthesis pathway, are important for insect growth and development. The whitefly TPS genes may be a main reason for the severe damage and may represent potential targets for the control of whiteflies.Results: In this study, we identified and cloned three TPS genes from B. tabaci MED and found that the BtTPS1 and BtTPS2 genes showed higher expression levels than the BtTPS3 gene. Then, RNA interference (RNAi) of BtTPS1 and BtTPS2 resulted in significant mortality and influenced the expression of related genes involved in energy metabolism and chitin biosynthesis in whitefly adults. Finally, the transgenic tobacco plants showed a significant effect on B. tabaci, and knockdown of BtTPS1 or BtTPS2 led to retarded growth and low hatchability in whitefly nymphs, and caused 90% mortality and decreased the fecundity in whitefly adults. Additionally, the transgenic tobacco with combinatorial RNAi of BtTPS1 and BtTPS2 showed a better efficacy against whiteflies than individual silencing. Conclusion: Our results suggest that silencing of the BtTPS genes can compromise the growth and development of whiteflies, offering not only a new option for whitefly control but also a secure and environmentally friendly management strategy.

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RNAi‐mediated silencing of ferritin genes in the brown planthopper Nilaparvata lugens affects survival, growth and female fecundity

Cited by 14 publications

References 63 publications

The molecular and functional characterization of ferritins in the hard tick Hyalomma rufipes

The molecular and functional characterization of ferritins in the hard tick Hyalomma rufipes

RNAi‐mediated silencing of an egg‐specific gene Nllet1 results in hatch failure in the brown planthopper

Silencing of the BtTPS genes by transgenic plant‐mediated RNAi to control Bemisia tabaciMED

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