<scp>RNAi</scp>‐mediated silencing of the autophagy‐related gene <scp><i>NlATG3</i></scp> inhibits survival and fecundity of the brown planthopper, <scp><i>Nilaparvata lugens</i></scp>

Ye, Chenglong; Feng, Yi; Yu, Feifei; Jiao, Qiqi; Wu, Jiangen; Ye, Zihong; Zhang, Pengjun; Sun, Chuanxin; Pang, Kun; Hao, Peiying; Yu, Xiaoping

doi:10.1002/ps.6507

Cited by 5 publications

(1 citation statement)

References 63 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To evaluate the RNAi effects of miRNA core genes on the survival of N. lugens , third‐instar nymphs were injected with dsRNAs. The injection of dsGFP served as a control and the same method as previously reported (Ye et al, 2021) was used. Three seedlings were used as three biological replicates, with each hosting 50 nymphs that had been injected with dsRNAs.…”

Section: Methodsmentioning

confidence: 99%

The microRNA pathway core genes are indispensable for development and reproduction in the brown planthopper, Nilaparvata lugens

Wang

Chen

Zhou

et al. 2023

Insect Molecular Biology

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small single‐stranded non‐coding RNAs involved in a variety of cellular events by regulating gene expression at the post‐transcriptional level. Several core genes in miRNA biogenesis have been reported to participate in a wide range of physiological events, in some insect species. However, the functional significance of miRNA pathway core genes in Nilaparvata lugens remains unknown. In the present study, we conducted a systematic characterisation of five core genes involved in miRNA biogenesis. We first performed spatiotemporal expression analysis and found that miRNA core genes exhibited similar expression patterns, with high expression levels in eggs and relatively high transcriptional levels in the ovaries and fat bodies of females. RNA interference experiments showed that injecting third‐instar nymphs with dsRNAs targeting the miRNA core genes, NlAgo1, NlDicer1, and NlDrosha resulted in high mortality rates and various degrees of body melanism, moulting defects, and wing deformities. Further investigations revealed that the suppression of miRNA core genes severely impaired ovarian development and oocyte maturation, resulting in significantly reduced fecundity and disruption of intercellular spaces between follicle cells. Moreover, the expression profiles of miR‐34‐5p, miR‐275‐3p, miR‐317‐3p, miR‐14, Let‐7‐1, and miR‐2a‐3p were significantly altered in response to the knockdown of miRNA core genes mixture, suggesting that they play essential roles in regulating miRNA‐mediated gene expression. Therefore, our results provide a solid theoretical basis for the miRNA pathway in N. lugens and suggest that the NlAgo1, NlDicer1, and NlDrosha‐dependent miRNA core genes are essential for the development and reproduction of this agricultural pest.

show abstract

Section: Methodsmentioning

confidence: 99%

The microRNA pathway core genes are indispensable for development and reproduction in the brown planthopper, Nilaparvata lugens

Wang

Chen

Zhou

et al. 2023

Insect Molecular Biology

View full text Add to dashboard Cite

show abstract

Disruption of transmission of plant pathogens in the insect order Hemiptera using recent advances in RNA interference biotechnology

Niebres,

Alviar

2023

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The review discusses current RNA interference (RNAi) biotechnological innovations for crop protection. Special attention is given to the management of insect pests in the order Hemiptera. This insect order has the most members of insects which transmit pathogens on economically important crops. It first briefly summarizes the characteristics of the insects in this order and the type of transmission mechanisms for viral and bacterial plant pathogens. RNAi products developed for other insects are also analyzed. Emphasis was made on the need for innovative management approaches to offset the threat of resistance by both the insect vector to insecticides and the pathogens to microbicides. Subsequently, the RNAi technology is described, which is particularly an ingenious method currently utilized in itself or in combination with other modern biotechnological innovations for managing important vector insects that could provide an additional powerful tool for use in integrated pest control programs.The requirements and recent advances for performing RNAi assays are detailed and an overview is given on how to produce cheaper double-stranded RNA as the main component of RNAi-based biopesticide. Examples of agricultural companies that use RNAi biotechnology in their product development were also discussed.

show abstract

Silencing suppressors of rice black‐streaked dwarf virus and rice stripe virus hijack the 26S proteasome of Laodelphax striatellus to facilitate virus accumulation and transmission

Liu

Zhu

Gao

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Rice black-streaked dwarf virus (RBSDV) is transmitted by small brown planthopper (Laodelphax striatellus [L. striatellus]) and causes devastating disease in rice. P9-1 has silencing suppression activity and is the key protein for viroplasm formation in RBSDV-infected plants and insects; however, its exact function is poorly understood.RESULTS: In this study, the P9-1 of RBSDV interacted with L. striatellus 26S proteasome subunit RPN8. RBSDV accumulation in L. striatellus increased after the 26S proteasome was disrupted by silencing the RPN8 expression. This finding indicated that L. striatellus 26S proteasome played a defense role against RBSDV infection by regulating RBSDV accumulation. Further investigations revealed that P9-1 could competitively bind to RPN8 with RPN7, thereby disrupting the assembly of 26S proteasome in L. striatellus and promoting the infection of RBSDV in insect vectors, and further affecting the transmission of the virus to rice by insect vectors. Similar to P9-1, rice stripe virus (RSV) NS2, a weak silencing suppressor, regulated virus accumulation and transmission by hijacking RPN8 to interfere with the function of 26S proteasome in L. striatellus.CONCLUSION: These results suggest that viruses promote their own infection via interfering with ubiquitination pathway of insect vectors, and this mechanism might be of universal importance. These findings provide a new insight into the mechanism of virus transmission in insect vectors.

show abstract

RNAi‐mediated silencing of the autophagy‐related gene NlATG3 inhibits survival and fecundity of the brown planthopper, Nilaparvata lugens

Cited by 5 publications

References 63 publications

The microRNA pathway core genes are indispensable for development and reproduction in the brown planthopper, Nilaparvata lugens

The microRNA pathway core genes are indispensable for development and reproduction in the brown planthopper, Nilaparvata lugens

Disruption of transmission of plant pathogens in the insect order Hemiptera using recent advances in RNA interference biotechnology

Silencing suppressors of rice black‐streaked dwarf virus and rice stripe virus hijack the 26S proteasome of Laodelphax striatellus to facilitate virus accumulation and transmission

Contact Info

Product

Resources

About